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Elucidating wood degradation mechanism of a standing tree pathogen

Research Project

Project/Area Number 16K18727
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionHokkaido University

Principal Investigator

Hori Chiaki  北海道大学, 工学研究院, 助教 (50722948)

Research Collaborator SANO yuzo  
TOBIMATSU yuki  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords木材腐朽菌 / ベッコウタケ / 樹木病理 / ゲノム解析 / 木材腐朽 / 立木加害 / 植物 / バイオマス / 植物バイオマス / 腐朽菌 / 微生物
Outline of Final Research Achievements

In this study, we first disclosed genome sequences of hardwood pathogen, Pereniporia fraxinea. Comparative genomic analysis showed that Pereniporia fraxinea has no difference in CAZy (enzymes related wood degradation) between other white rot fungi. Secreted enzymes from Pereniporia fraxinea grown on culture media including wood, cellulose or glucose as a sole carbon source were analyzed and compared with model white rot fungus Phanerochaete chrysosporium. As a result, this fungus secreted an unique cellulase and lots of laccases. These enzymes might be useful for tree protection and plant biomass utilization in the future.

Academic Significance and Societal Importance of the Research Achievements

ベッコウタケは北海道大学構内をはじめ日本中で倒木被害を引き起こしており、倒木による物損事故などが社会的に大きく取り上げられている。しかしながら予防策はなく、被害が確認できてから伐採するなど対処療法が行われているが、子実体に気づきにくいなど問題点が多い。そこで本研究では予防法に至る知見を得るため、ベッコウタケ菌の特徴的な植物バイオマス分解機構や樹木が生産する防御物質の分解メカニズムを明らかにした。この知見は、樹木保護や植物バイオマス分解利用に将来的に役立つ可能性があると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (44 results)

All 2019 2018 2017 2016 Other

All Int'l Joint Research (3 results) Journal Article (16 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 16 results,  Open Access: 4 results) Presentation (23 results) (of which Int'l Joint Research: 4 results) Remarks (2 results)

  • [Int'l Joint Research] Forest Products Laboratory(米国)

    • Related Report
      2018 Annual Research Report
  • [Int'l Joint Research] USDA-FPL(米国)

    • Related Report
      2017 Research-status Report
  • [Int'l Joint Research] 米国農務省林産研究所(米国)

    • Related Report
      2016 Research-status Report
  • [Journal Article] High-cell density culture of poly(lactate-co-3-hydroxybutyrate)-producing Escherichia coli by using glucose/xylose-switching fed-batch jar fermentation.2019

    • Author(s)
      Hori C, Yamazaki T, Ribordy G, Takisawa K, Matsumoto K, Ooi T, Zinn M, Taguchi S.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 印刷中 Issue: 6 Pages: 721-725

    • DOI

      10.1016/j.jbiosc.2018.11.006

    • NAID

      40021920356

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Ribulose-1,5-bisphosphate carboxylase/oxidase (RuBisCO)-mediated de novo synthesis of glycolate-based polyhydroxyalkanoate in Escherichia coli.2019

    • Author(s)
      Matsumoto K, Saito J, Yokoo T, Hori C, Nagata A, Kudoh Y, Ooi T, Taguchi S.
    • Journal Title

      J. Biosci. Bioeng.

      Volume: 印刷中 Issue: 3 Pages: 302-306

    • DOI

      10.1016/j.jbiosc.2019.03.002

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genomes of neutrophilic sulfur-oxidizing chemolithoautotrophs representing 9 proteobacterial species from 8 genera.2019

    • Author(s)
      Watanabe T, Kojima H, Umezawa K, Hori C, Takasuka T, Kato Y, Fukui M.
    • Journal Title

      Frontiers in Microbiol.

      Volume: 印刷中

    • DOI

      10.3389/fmicb.2019.00316

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Structural Integrity of Lignin Is Crucial for Resistance against Striga hermonthica Parasitism in Rice2019

    • Author(s)
      Mutuku J. Musembi、Cui Songkui、Hori Chiaki、Takeda Yuri、Tobimatsu Yuki、Nakabayashi Ryo、Mori Tetsuya、Saito Kazuki、Demura Taku、Umezawa Toshiaki、Yoshida Satoko、Shirasu Ken
    • Journal Title

      Plant Physiology

      Volume: 179 Issue: 4 Pages: 1796-1809

    • DOI

      10.1104/pp.18.01133

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Multi-omic analyses of extensively decayed Pinus contorta reveal expression of diverse array of lignocellulose degrading enzymes.2018

    • Author(s)
      Hori C, Gaskell J, Cullen D, Sabat G, Stewart PE, Lail K, Peng Y, Barry K, Grigoriev IV, Kohler A, Fauchery L, Martin F, Zeiner CA, Bhatnagar JM.
    • Journal Title

      Appl. Environ. Microbiol.

      Volume: 84 Issue: 20

    • DOI

      10.1128/aem.01133-18

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Protein components of water extracts from fruiting bodies of the reishi mushroom Ganoderma lucidum contribute to the production of functional molecules2018

    • Author(s)
      Kumakura Kei、Hori Chiaki、Matsuoka Hiroki、Igarashi Kiyohiko、Samejima Masahiro
    • Journal Title

      Journal of the Science of Food and Agriculture

      Volume: 99 Issue: 2 Pages: 529-535

    • DOI

      10.1002/jsfa.9211

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Secretome analysis of the basidiomycete Phanerochaete chrysosporium grown on ammonia-treated lignocellulosic biomass from birch wood.2018

    • Author(s)
      Sakuragi S, Hori C, Igarashi K, Samejima M.
    • Journal Title

      J. Wood Sci.

      Volume: 64 Issue: 6 Pages: 845-853

    • DOI

      10.1007/s10086-018-1770-4

    • NAID

      40021752969

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] In Vitro Analysis of d-Lactyl-CoA-Polymerizing Polyhydroxyalkanoate Synthase in Polylactate and Poly(lactate-co-3-hydroxybutyrate) Syntheses2018

    • Author(s)
      Ken’ichiro Matsumoto, Midori Iijima, Chiaki Hori, Camila Utsunomia, Toshihiko Ooi, and Seiichi Taguchi
    • Journal Title

      Biomacromolecules

      Volume: 19 Issue: 7 Pages: 28892895-28892895

    • DOI

      10.1021/acs.biomac.8b00454

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Site-directed saturation mutagenesis of polyhydroxylalkanoate synthase for efficient microbial production of poly[(R)-2-hydroxybutyrate]2018

    • Author(s)
      Hori C, Oishi K, Matsumoto K, Taguchi S, Ooi T.
    • Journal Title

      J Biosci Bioeng.

      Volume: 印刷中 Issue: 6 Pages: 632-636

    • DOI

      10.1016/j.jbiosc.2017.11.013

    • NAID

      210000005688

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Dynamic changes of intracellular monomer levels regulate block sequence of polyhydroxyalkanoates in engineered Escherichia coli.2018

    • Author(s)
      Matsumoto K, Hori C, Fujii R, Takaya M, Ooba T, Ooi T, IsonoT, Satoh T, Taguchi S.
    • Journal Title

      Biomacromolecules

      Volume: 19 Issue: 2 Pages: 662-671

    • DOI

      10.1021/acs.biomac.7b01768

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Changes in the Secondary Xylem of the Living Stem of Four Tree Species in Response to Inoculation with <i>Perenniporia fraxinea</i>2018

    • Author(s)
      上田幹朗、堀 千明、玉井裕、山岸祐介, 宮本敏澄, 佐野雄三
    • Journal Title

      Mokuzai Gakkaishi

      Volume: 64 Issue: 1 Pages: 1-9

    • DOI

      10.2488/jwrs.64.1

    • NAID

      130006321460

    • ISSN
      0021-4795, 1880-7577
    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Synthesis of lactate (LA)-based poly(ester-urethane) using hydroxyl-terminated LA-based oligomers from a microbial secretion system.2017

    • Author(s)
      Utsunomia, C., Saito, T., Matsumoto, K., Hori, C., Isono, T., Satoh, T., Taguchi, S.
    • Journal Title

      J. Polym. Res.

      Volume: 24 Issue: 10 Pages: 167-172

    • DOI

      10.1007/s10965-017-1325-1

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Investigation of the Escherichia coli membrane transporters involved in the secretion of D-lactate-based oligomers by loss-of-function screening2017

    • Author(s)
      Utsunomia, C., Hori, C., Matsumoto, K., Taguchi, S.
    • Journal Title

      J Biosci Bioeng.

      Volume: 124 Issue: 6 Pages: 635-640

    • DOI

      10.1016/j.jbiosc.2017.06.018

    • NAID

      40021420068

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Microbial secretion of lactate-enriched oligomers for efficient conversion into lactide: A biological shortcut to polylactide2017

    • Author(s)
      Utsunomia, C., Matsumoto, K., Date, S., Hori, C., Taguchi, S.
    • Journal Title

      J Biosci Bioeng.

      Volume: 124 Issue: 2 Pages: 204-208

    • DOI

      10.1016/j.jbiosc.2017.03.002

    • NAID

      40021295260

    • Related Report
      2017 Research-status Report 2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A protease/peptidase from culture medium of Flammulina velutipes that acts on arabinogalactan-protein2017

    • Author(s)
      Yoshimi, Y., Sugawara, Y., Hori, C., Igarashi, K., Kaneko, S., Tsumuraya, Y., and Kotake, T.
    • Journal Title

      Biosci. Biotechnol. Biochem.

      Volume: 81 Issue: 3 Pages: 475-481

    • DOI

      10.1080/09168451.2016.1258985

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Heterologous overexpression of poplar SnRK2 genes enhanced salt stress tolerance in Arabidopsis thaliana.2016

    • Author(s)
      Song X., Yu X., Hori C., Demura T., Ohtani M., Zhuge Q
    • Journal Title

      Frontiers in Plant Science

      Volume: 7 Pages: 612-612

    • DOI

      10.3389/fpls.2016.00612

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] マルチメタオミクス解析によって明らかになった自然環境下での木材分解の多様性2019

    • Author(s)
      堀千明、ジル・ガスケル、ダニエル・カレン、大井俊彦、松本謙一郎
    • Organizer
      日本農芸化学会2019大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 腐朽された樹木のメタ・オミクス解析 ~環境下で腐朽を担う微生物群と酵素群が明らかに~2019

    • Author(s)
      堀千明、大井俊彦、松本謙一郎、ジル・ガスケル、ダニエル・カレン
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 針葉樹分解性白色腐朽菌Phlebiopsis giganteaにおける抽出成分の分解性解析2019

    • Author(s)
      岩田茉奈、堀千明、大井俊彦、松本謙一郎、佐野雄三
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] ハリエンジュが生産する生体防御物質に対する立木加害性木材腐朽菌ベッコウタケの応答2019

    • Author(s)
      松本壘、堀千明、大井俊彦、松本謙一郎、宮本敏澄、重富顕吾、佐野雄三
    • Organizer
      第69回日本木材学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 木材腐朽菌ベッコウタケと宿主木ハリエンジュの相互作用解析2018

    • Author(s)
      松本壘、堀千明、宮本敏澄、重富顕吾、佐野雄三、大井俊彦、松本謙一郎
    • Organizer
      日本生物工学会北日本支部札幌シンポジウム
    • Related Report
      2018 Annual Research Report
  • [Presentation] 組換え大腸菌による乳酸ベースオリゴマーの分泌生産と高分子合成への応用2018

    • Author(s)
      松本謙一郎、ウツノミアカミラ、堀千明、田口精一
    • Organizer
      2018年度日本農芸化学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 森林害虫共生微生物が生産する菌体外酵素の解析2018

    • Author(s)
      堀千明, SONG Ruopu, MINKOFF B. Benjamin, 飛松裕基, 高須賀太一
    • Organizer
      第68回日本木材学会
    • Related Report
      2017 Research-status Report
  • [Presentation] Effects of plant defensive substances on growth and CAZy production of wood decay fungus Pereniporia fraxinea that infects living tree2018

    • Author(s)
      Hori C, Matsumoto R, Miyamoto T, Shigetomi K, Sano Y, Ooi T, Matsumoto K
    • Organizer
      International Symposium on Biomass Refinery: From Biomass Crops to Chemicals and Fuels
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Dynamic changes of intracellular monomer levels regulate block sequence of polyhydroxyalkanoates in engineered Escherichia coli2018

    • Author(s)
      Fujii R, Matsumoto K, Hori C, Takaya M, Ooba T, Taguchi S, Ooi T, Isono T, Satoh T
    • Organizer
      International Symposium on Biomass Refinery: From Biomass Crops to Chemicals and Fuels
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Direct secretory production of D-lactate oligomers by engineered Escherichia coli: a shortcut in the process of polylactide production2017

    • Author(s)
      Utsunomia C, Matsumoto K, Hori C, Taguchi S
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 抗菌ペプチド・アピデシンおよび高活性変異体の作用機構解析2017

    • Author(s)
      川上駿、山崎蔵亨、三吉大地、大井俊彦、橋本茂樹、堀千明、田口精一、松本謙一郎
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Lactyl-CoA 重合における乳酸重合酵素の反応機構解析2017

    • Author(s)
      飯島翠、松本謙一郎、堀千明、田口精一、大井俊彦
    • Organizer
      第69回日本生物工学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] きのこゲノム情報を利用した植物バイオマス分解の効率化2017

    • Author(s)
      堀 千明、松本謙一郎、田口精一
    • Organizer
      第6回JACI/GSCシンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Application of D-lactate-based oligomers secreted by engineered Escherichia coli on polylactide production2017

    • Author(s)
      Camila Utsunomia, Ken’ichiro Matsumoto, Chiaki Hori, and Seiichi Taguchi
    • Organizer
      第6回JACI/GSCシンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] バイオマスを原料とした多元ポリ乳酸の微生物生産2017

    • Author(s)
      飯島翠、滝沢憲治、門屋亨介、堀千明、松本謙一郎、大井俊彦、田口精一
    • Organizer
      第6回JACI/GSCシンポジウム
    • Related Report
      2017 Research-status Report
  • [Presentation] Bacterial production of glycolate-based polymers with hydrolytic degradability2017

    • Author(s)
      Saito J, Shiba T, Hiraide Y, Hori C, Matsumoto K, Ooi T, Taguchi S
    • Organizer
      JSPS-Core-to-Core Program:Japan-South-East Asia Collaboration Hub of Bioplastics Study
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] 種々の樹木成分で生育させた白色腐朽菌Phanerochaete chrysosporiumの細胞外プロテオーム解析2016

    • Author(s)
      糀谷紗季、鈴木梨央、酒井杏匠、高須賀太一、堀 千明、志水元亨、加藤雅士
    • Organizer
      第16回糸状菌分子生物学会
    • Place of Presentation
      京都大学宇治キャンパス(宇治市)
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 比較プロテオミクスによるアラスカ永久凍土氷楔由来放線菌の休眠状態の代謝生理解析2016

    • Author(s)
      池晃祐、長倉 美琴、高須賀 太一、堀 千明、北川 航、加藤 創一郎、曾根 輝雄、鎌形 洋一
    • Organizer
      日本微生物生態学会第31回大会
    • Place of Presentation
      横須賀市文化会館(横須賀市)
    • Year and Date
      2016-10-22
    • Related Report
      2016 Research-status Report
  • [Presentation] Characterization of biomass degradation by fungal and bacterial enzymes from wood devastating insect symbionts2016

    • Author(s)
      HORI Chiaki, SONG Ruopu, MINKOFF B. Benjamin, TOBIMATSU Yuki, TAKASUKA Taichi
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      仙台国際センター/東北大学川内北キャンパス(仙台市)
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
  • [Presentation] Alteration of cell wall structures and gene regulations in Populus trichocarpa under abiotic stress.2016

    • Author(s)
      Xiang Yu, Chiaki Hori, Misato Ohtani, Jun Kikuchi, Taku Demura
    • Organizer
      XIV Cell Wall Meeting
    • Place of Presentation
      ハニア、クレタ、ギリシャ
    • Year and Date
      2016-06-12
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] ベッコウタケ接種に対する生立木樹幹二次木部の反応2016

    • Author(s)
      上田幹朗,堀 千明,山岸祐介,宮本敏澄,玉井 裕,佐野雄三
    • Organizer
      日本木材学会北海道支部 第48回研究発表会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2016 Research-status Report
  • [Presentation] 発現遺伝子のオミックス解析によるブナ成木の衰退度の評価法~トランスクリプトーム VS.プロテオーム~2016

    • Author(s)
      斎藤秀之、高須賀太一、堀 千明、神村章子、小林壱徳久、和田尚之、齋藤央嗣、谷脇徹、相原敬次、小倉淳、瀬々潤
    • Organizer
      第65回北方森林学会
    • Place of Presentation
      札幌コンベンションセンター(札幌市)
    • Related Report
      2016 Research-status Report
  • [Presentation] アラスカ永久凍土氷楔由来放線菌の休眠細胞のプロテオーム解析2016

    • Author(s)
      池晃祐、長倉 美琴、高須賀 太一、堀 千明、北川 航、加藤 創一郎、曾根 輝雄、鎌形 洋一
    • Organizer
      2016年度生物工学会北日本支部会
    • Place of Presentation
      北海道大学工学部 (札幌市)
    • Related Report
      2016 Research-status Report
  • [Remarks] バイオ分子工学研究室ホームページ

    • URL

      http://labs.eng.hokudai.ac.jp/labo/seika/

    • Related Report
      2017 Research-status Report
  • [Remarks] 研究室ホームページ

    • URL

      http://labs.eng.hokudai.ac.jp/labo/seika/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2022-02-21  

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