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Functional characterization of novel transcription factors that regulate distribution of cortical microtubules in wood fibers in poplar

Research Project

Project/Area Number 16K18734
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Wood science
Research InstitutionForest Research and Management Organization

Principal Investigator

Takata Naoki  国立研究開発法人森林研究・整備機構, 森林総合研究所 森林バイオ研究センター, 主任研究員 等 (90605544)

Research Collaborator Awano Tatsuya  
Yamagishi Yusuke  
Tobimatsu Yuki  
Suzuki Shiro  
Lam Pui Ying  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords組織構造 / 表層微小管 / 二次壁 / 木部繊維 / 転写フィードバックループ
Outline of Final Research Achievements

In the present study, we characterized transcription factors, TF26 and TF26b, which were identified as novel regulators of cortical microtubule distribution in poplar. TF26 and the paralogous gene, TF26b, were preferentially expressed in the xylem tissues of poplar. Overexpression of TF26 enhanced cell wall formation in wood fiber cells. We generated double knockout poplars, tf26 tf26b. Histological analysis of xylem tissues showed that secondary cell wall of wood fibers was depressed in the knockout mutants. Microarray analysis and quantitative real-time PCR analysis using TF26 overexpressors and double knockout mutants demonstrated that TF26 and TF26b form a positive transcriptional feedback loop with NST/SND orthologues, master regulators of secondary cell wall formation in wood fibers.

Academic Significance and Societal Importance of the Research Achievements

本研究で同定した転写因子(TF26遺伝子およびTF26b遺伝子)は、木部繊維の二次壁形成のマスター転写因子NST/SND遺伝子(VNS09およびVNS10)と正の転写フィードバックループを形成している。新たに発見した本制御機構は、木部繊維が二次壁を厚く堆積するためのシステムであると推察される。本研究成果により、支持細胞(木部繊維)が巨大な樹体を支えるために細胞壁を厚く発達させ物理的強度を獲得する分子メカニズムの一端を明らかにすることができた。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2019 2018 2017

All Journal Article (3 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 3 results) Presentation (10 results) (of which Int'l Joint Research: 3 results,  Invited: 2 results) Book (1 results)

  • [Journal Article] Populus NST/SND orthologs are key regulators of secondary cell wall formation in wood fibers, phloem fibers and xylem ray parenchyma cells2019

    • Author(s)
      Takata Naoki, Awano Tatsuya, Nakata Miyuki T, Sano Yuzou, Sakamoto Shingo, Mitsuda Nobutaka, Taniguchi Toru
    • Journal Title

      Tree Physiology

      Volume: 印刷中 Issue: 4 Pages: 514-525

    • DOI

      10.1093/treephys/tpz004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Change in lignin structure,but not in lignin content,in transgenic poplar overexpressing the rice master regulator of secondary cell wall biosynthesis.2018

    • Author(s)
      Nuoendagula, Yukiko Tsuji, Naoki Takata, Shingo Sakamoto, Akiko Nakagawa‐Izumi, Toru Taniguchi, John Ralph, Nobutaka Mitsuda, Shinya Kajita
    • Journal Title

      Physiologia Plantarum

      Volume: 印刷中 Issue: 2 Pages: 170-182

    • DOI

      10.1111/ppl.12684

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] The Arabidopsis NST3/SND1 promoter is active in secondary woody tissue in poplar.2017

    • Author(s)
      Naoki Takata, Shingo Sakamoto, Nobutaka Mitsuda, Toru Taniguchi
    • Journal Title

      Journal of Wood Science

      Volume: 63 Pages: 396-400

    • NAID

      40021280437

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Presentation] R3 MYB遺伝子の過剰発現によるポプラ木質の酵素糖化性の改良2019

    • Author(s)
      高田直樹, 堀千明, 松本謙一郎, ラム イン プイ, 飛松裕基, 永野聡一郎
    • Organizer
      第60回日本植物生理学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] CRISPR/Cas9 approaches to understand secondary cell wall formation in wood fibers in poplar.2019

    • Author(s)
      Naoki Takata
    • Organizer
      Bioengineering of Lignocellulose for Clean Energy Production: Perspectives and Opportunities
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Wood formation and engineering in poplar.2019

    • Author(s)
      Naoki Takata
    • Organizer
      JST-ALCA the 2nd Symposium of Plant Cell Wall Engineering ―Toward Low Carbon Society―
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] NST/SND-independent secondary cell wall formation in wood fibers in the vicinity of vessel element in poplar.2018

    • Author(s)
      Naoki Takata, Tatsuya Awano, Yuzou Sano, Miyuki T. Nakata, Shingo Sakamoto, Nobutaka Mitsuda, Toru Taniguchi
    • Organizer
      International Symposium on Forest Tree Molecular Biology and Biotechnology 2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] ポプラNST四重変異体の組織構造2018

    • Author(s)
      高田直樹, 粟野達也, 佐野雄三, 中田未友希, 坂本真吾, 光田展隆, 谷口亨
    • Organizer
      植物細胞壁研究者ネットワーク定例研究会(2018)
    • Related Report
      2018 Annual Research Report
  • [Presentation] ゲノム編集技術により見出された新たな木部繊維の存在2018

    • Author(s)
      高田直樹, 粟野達也, 中田未友希, 坂本真吾, 光田展隆, 佐野雄三, 谷口亨
    • Organizer
      第68回日本木材学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Feedback regulation in secondary cell wall thickening in poplar.2018

    • Author(s)
      高田直樹, 粟野達也, 朽名夏麿, 谷口亨
    • Organizer
      第59回日本植物生理学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ポプラにおいて見出された二次壁形成の正の転写フィードバックループ2017

    • Author(s)
      高田直樹, 谷口亨
    • Organizer
      第67回日本木材学会大会
    • Place of Presentation
      九州大学箱崎キャンパス(福岡県福岡市)
    • Year and Date
      2017-03-17
    • Related Report
      2016 Research-status Report
  • [Presentation] Positive feedback loop in secondary cell wall formation in poplar.2017

    • Author(s)
      Takata Naoki, Taniguchi Toru
    • Organizer
      第58回日本植物生理学会大会
    • Place of Presentation
      鹿児島大学郡元キャンパス(鹿児島県鹿児島市)
    • Year and Date
      2017-03-16
    • Related Report
      2016 Research-status Report
  • [Presentation] イネの遺伝子を使ってポプラの木質バイオマスの増産に成功2017

    • Author(s)
      高田直樹, 谷口亨
    • Organizer
      第15回環境研究シンポジウム
    • Related Report
      2017 Research-status Report
  • [Book] Survival Strategies in Extreme Cold and Desiccation, Adaptation Mechanisms and Their Applications: Mechanism of overwintering in trees.2018

    • Author(s)
      Keita Arakawa, Jun Kasuga, Naoki Takata
    • Total Pages
      409
    • Publisher
      Springer
    • Related Report
      2018 Annual Research Report

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Published: 2016-04-21   Modified: 2020-03-30  

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