Project/Area Number |
16K18746
|
Research Category |
Grant-in-Aid for Young Scientists (B)
|
Allocation Type | Multi-year Fund |
Research Field |
Aquatic bioproduction science
|
Research Institution | Japan International Research Center for Agricultural Sciences |
Principal Investigator |
Okutsu Tomoyuki 国立研究開発法人国際農林水産業研究センター, その他部局等, 研究員(移行) (40456322)
|
Research Collaborator |
MARUYAMA Kyonoshin 国際農林水産業研究センター, 水産領域, 主任研究員
|
Project Period (FY) |
2016-04-01 – 2018-03-31
|
Project Status |
Completed (Fiscal Year 2017)
|
Budget Amount *help |
¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
|
Keywords | バナメイエビ / 卵巣成熟 / 分子マーカー / マイクロアレイ / クルマエビ科 / 水産増養殖 |
Outline of Final Research Achievements |
We found 66,166 genes encoding a certain protein among 72,348 unigene sequences in whiteleg shrimp, Litopenaeus vannamei, obtained from NCBI database, and revealed their amino acid sequences. The microarray probes were designed from the above-mentioned 72,348 unigene sequences using eArray software. A comparative transcriptomic analysis using the array revealed GILT and NP1 gene showed specific expression in mature and undeveloped ovary in L. vannamei. The microarray developed in this study can be a useful tools to uncover the molecular mechanisms in ovarian development in Penaeid shrimp species.
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