Comprehensive Omics analysis and functional characterization of the role of nervous system in the larval settlement of barnacle cyprids

• Project/Area Number: 16K18749
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Akita Prefectural University
• Principal Investigator: Wong Yue Him 秋田県立大学, 生物資源科学部, 特任助教 (80769547)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: フジツボ / トランスクリプトーム / 神経ペプチド / キプリス幼生 / 付着防除 / Neuropeptides / Peptide hormones / Immunohistochemistry / Evolution / Larvae nervous system / SIFamide / neuropeptide / In silico peptide mining / cyprids / 生理 / ﾌｼﾞﾂﾎﾞ / 神経 / 幼生付着 / NGS

Outline of Final Research Achievements

The transcripts of 11 classes of neuropeptides, including allatostatin, tachykinin, sulfukinin, RFamide, SIFamide, insulin-like peptide, bursicon, pigment dispersal hormone, eclosion hormone, glycoprotein hormone, and calcitonin, were recovered from the Megabalanus rosa transcriptome and in-silico mining method. Immunostaining of three neuropeptides, SIFamide, FGLamide (A-type allatostatin) and FMRFamide, reveal the expression of these NPs in the cyprid larval nervous system. Comparative analyses reveal the conserved amino acid sequences of SIFamide, FGLamide among crustaceans, but surprisingly mutated NP sequences of the closely related parasitic barnacle clade.　SIFamide was found to be highly expressed in the cyprid stage of both M.rosa and the parasitic barnacle Succulina yatsui. These results serve as the basis for the functional behavioral studies of NPs, especially SIFamide, in the cypris larvae of M.rosa and other barnacle species.

Research Products

• [Int'l Joint Research] University of Science and Technology(Hong Kong, China)
• [Int'l Joint Research] Academia Sinica(Taiwan)
• [Int'l Joint Research] HKUST(Hong Kong)
• [Journal Article] Identification, characterization, and larval biology of a rhizocephalan barnacle, Sacculina yatsui Boschma, 1936, from northwestern Japan (Cirripedia: Sacculinidae) (2018)
  • Author(s): Kobayashi Momoko, Wong Yue-Him, Oguro-Okano Mieko, Dreyer Niklas, H; Jens T., Yoshida Ryuta and Okano Keiju
  • Journal Title: Journal of Crustacean Biology Volume: 38 Issue: 3 Pages: 329-340
  • DOI: 10.1093/jcbiol/ruy020
  • Peer Reviewed / Int'l Joint Research
• [Presentation] Identification of barnacle shell proteins by transcriptome and proteomic approaches (2017)
  • Author(s): Yue Him Wong, Noriaki Ozaki, Wei-Pang Zhang, Jin Sun, Erina Yoshimura, Mieko Oguro-Okano, Yasuyuki Nogata, Hsiu-Chin Lin, Benny KK Chan, Pei-Yuan Qian, Keiju Okano
  • Organizer: The 14th International Symposium on Biomineralization (BIOMIN XIV) From Molecular and Nano-structural Analyses to Environmental Science
  • Int'l Joint Research / Invited
• [Presentation] アカフジツボの幼稚体における殻形成過程の解析 (2017)
  • Author(s): Yue Him Wong
  • Organizer: 2018年度日本付着生物学会総会・研究集会
• [Presentation] Integrating "Omics" approaches and comparative analysis in the study of novel proteins in fouling organisms: a case study on Megabalanus rosa shell formation (2017)
  • Author(s): Yue Him Wong, Yasuyuki Nogata, and Mieko Oguro-Okano, Jin Sun, Wei-Pang Zhang, Hsiu-Chin Lin, Benny K.K. Chan, Pei-Yuan-Qian, Keiju Okano
  • Organizer: 日本付着生物学会シンポジウム
  • Invited
• [Presentation] 寄生性フジツボのインテルナに特異的高発現するペプチドホルモンの探索 (2017)
  • Author(s): Wong Yue Him、沼澤涼子、小林桃子、筒井直昭、J. T. Joeg、小黒-岡野美枝子、 岡野桂樹
  • Organizer: 第19回 マリンバイオテクノロジー学会
• [Book] Barnacles: Recent Progress in Biology and Antifouling (2018)
  • Author(s): Okano Keiju, Wong Yue-Him, Takahashi Yayoi, Kikuchi Ayako, Nogata Yasuyuki, and Oguro-Okano Mieko
  • Publisher: Nova Science Publishers, Inc
  • ISBN: 9781536134445