Elucidation of molecular mechanisms of hematopoiesis in teleost fish

• Project/Area Number: 16K18752
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Aquatic life science
• Research Institution: Nihon University
• Principal Investigator: KATAKURA Fumihiko 日本大学, 生物資源科学部, 助教 (10756597)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 魚類 / 造血因子 / 造血幹／前駆細胞 / 魚類造血 / サイトカイン / 造血微小環境 / 造血 / 造血組織間質細胞 / 免疫学

Outline of Final Research Achievements

To elucidate molecular mechanisms of hematopoiesis in teleost fish, we tried to identify and characterize various hematopoietic growth factors regulating development of all blood cell lineages in common carp. Carp TPO was revealed to be a thrombopoietic cytokine that promotes thrombocytic colony-formation from kidney cells. We found four paralogs of carp G-CSF that have different functions regarding development, trafficking and activation of neutrophils. We further identified a novel cytokine that promotes development of basophils in carp. Homologs of Kit ligand, CSF-1, IL-7 and Notch ligands were cloned from carp.

Academic Significance and Societal Importance of the Research Achievements

魚類の全ての血球系列の造血を制御するサイトカインの同定に成功した。本研究の進展により、魚類の生理・免疫機構が包括的に明らかにされ、魚類養殖において問題となる感染症の制御にむけた診断法や予防法などの開発の基盤となることが期待される。また魚類は哺乳類との共通祖先形質を多く残しており造血研究モデルとして有用であると考えられ、系統発生学的視点から脊椎動物の造血システムの普遍性と多様性を理解することにつながる。

Research Products

• [Int'l Joint Research] Wageningen University & Research(オランダ)
• [Int'l Joint Research] Wageningen University(オランダ)
• [Int'l Joint Research] Wageningen University(オランダ)
• [Journal Article] Paralogs of Common Carp Granulocyte Colony-Stimulating Factor (G-CSF) Have Different Functions Regarding Development, Trafficking and Activation of Neutrophils (2019)
  • Author(s): Katakura Fumihiko、Nishiya Kohei、Wentzel Annelieke S.、Hino Erika、Miyamae Jiro、Okano Masaharu、Wiegertjes Geert F.、Moritomo Tadaaki
  • Journal Title: Frontiers in Immunology Volume: 10 Pages: 255-255
  • DOI: 10.3389/fimmu.2019.00255
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Thrombopoietin (TPO) induces thrombocytic colony formation of kidney cells synergistically with kit ligand A and a non-secretory TPO variant exists in common carp (2018)
  • Author(s): Fumihiko Katakura, Yuji Sugie, KiyotakaHayashi, Kohei Nishiya, Jiro Miyamae, Masaharu Okano, Teruyuki Nakanishi, Tadaaki Moritomo
  • Journal Title: Developmental & Comparative Immunology Volume: 84 Pages: 327-336
  • DOI: 10.1016/j.dci.2018.03.005
  • Peer Reviewed / Open Access
• [Journal Article] Isolation and characterization of hematopoietic stem cells in teleost fish (2016)
  • Author(s): Isao Kobayashi, Fumihiko Katakura, Tadaaki Moritomo
  • Journal Title: Developmental and Comparative Immunology Volume: 58 Pages: 86-94
  • DOI: 10.1016/j.dci.2016.01.003
  • NAID: 120005771110
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Presentation] Using cell penetrating peptide for intracellular delivery of Edwardsiella tarda OmpA and study the immune response in Carassius auratus langsdorfii and Carassius auratus. (2019)
  • Author(s): Chia-Jung Chang，舛廣善和，難波亜紀，間野伸宏，片倉文彦，森友忠昭
  • Organizer: 平成31年度 日本魚病学会春季大会
• [Presentation] PARALOGS OF CARP GRANULOCYTE COLONY-STIMULATING FACTOR (G-CSF) HAVE DIFFERENT FUNCTIONS WITH REGARD TO DEVELOPMENT, TRAFFICKING AND ACTIVATION OF NEUTROPHILS (2018)
  • Author(s): Fumihiko Katakura, Kohei Nishiya, Annelieke S. Wentzel, Erika Hino, Jiro Miyamae, Masaharu Okano, Geert F. Wiegertjes, Tadaaki Moritomo
  • Organizer: 14th Congress of the International Society of Developmental & Comparative Immunology
  • Int'l Joint Research
• [Presentation] IDENTIFICATION OF NEUTROPHILIC PROGENITORS USING A COLONY ASSAY SYSTEM IN THE PRESENCE OF RECOMBINANT CARP GRANULOCYTE COLONY-STIMULATING FACTOR PARALOGS (2018)
  • Author(s): Kohei Nishiya, Fumihiko Katakura, Jiro Miyamae, Masaharu Okano, Tadaaki Moritomo
  • Organizer: 14th Congress of the International Society of Developmental & Comparative Immunology
  • Int'l Joint Research
• [Presentation] コイKit ligand aは造血前駆細胞の増殖・維持を促す (2018)
  • Author(s): 西谷 広平，片倉 文彦，宮前 二朗，岡野 雅春，森友 忠昭
  • Organizer: 日本比較免疫学会第30回学術集会
• [Presentation] コイinterleukin-5様分子の同定および機能解析 (2018)
  • Author(s): 澤田 真衣、西谷 広平、岡野 雅春、宮前 二朗、Johannes M. Dijkstra、片倉 文彦、森友 忠昭
  • Organizer: 日本比較免疫学会第30回学術集会
• [Presentation] コイ顆粒球コロニー刺激因子GCSFの造血活性の検討 (2017)
  • Author(s): 西谷広平，日野エリカ，宮前二朗，岡野雅春，片倉文彦，森友忠昭
  • Organizer: 日本動物学会第88回富山大会
• [Presentation] コイ顆粒球コロニー刺激因子は好中球の造血および遊走を促す (2017)
  • Author(s): 片倉 文彦，西谷 広平，日野 エリカ，Annelieke Wentzel，宮前 二朗，岡野 雅春，Geert Wiegertjes，森友 忠昭
  • Organizer: 日本比較免疫学会第29回学術集会
• [Presentation] 種々の造血因子を用いたコロニー形成培養法によるコイ造血前駆細胞の同定 (2016)
  • Author(s): 片倉文彦，西谷広平，日野エリカ，宮前二朗，森友忠昭
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] コイT細胞レセプタ ー(TCR)αδ遺伝子領域のゲノム解析 (2016)
  • Author(s): 岡野雅春，宮前二朗，片倉文彦，森長真一，森友忠昭
  • Organizer: 第159回日本獣医学会学術集会
  • Place of Presentation: 日本大学（神奈川県藤沢市）
  • Year and Date: 2016-09-06
• [Presentation] Identification and functional characterization of two granulocyte colony-stimulating factors of common carp (2016)
  • Author(s): Katakura F., Hino E., Nishiya K., Miyamae J., Moritomo T.
  • Organizer: 2nd international conference of fish and shellfish immunology
  • Place of Presentation: Portland, Maine, USA
  • Year and Date: 2016-06-26
  • Int'l Joint Research