| Project/Area Number |
16K18795
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Kyoto University |
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Principal Investigator |
Keiko Shindo 京都大学, ウイルス・再生医科学研究所, 特定研究員 (00771312)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKANO Masahiro 京都大学ウイルス, 再生医科学研究所, 助教 (90456997)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | インフルエンザウイルス / 転写 / 複製 / RNP / リコンビナントRNP |
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| Outline of Final Research Achievements |
Influenza virus genome is composed of multiple copies of NP and a RNP polymerase complex, forming a ribonucleoprotein complex (RNP), which is responsble for transcription and replication of the viral genome. The RNPs have flexible double-stranded helices, suggesting that conformational changes may take place during the transcription and replication. However, conformation of RNPs in the functional state remains unknown. Here, to visualize conformation of RNPs during transcription and replication, we have tried to establish a system by combining in vitro RNA synthesis and high speed atomic force microscopy (AFM) observation. We have successflly purify a recombinant RNP, which contains 248nt-length minigenome derived from NS segment and forms ring-like structures. Although we confirmed that the recombinant RNPs have transcription and replication activity by RI, we could not observe growing RNAs produced from the recombinant RNPs under the AFM observation.
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