Reconstitution of a novel recombinant Marek's disease virus as a vector vaccine against poultry red mites
| Project/Area Number |
16K18798
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Veterinary medical science
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| Research Institution | Hokkaido University |
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Principal Investigator |
Shiro Murata 北海道大学, 獣医学研究科, 助教 (10579163)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | ワクモ / 組換えワクチン / マレック病 / マレック病ウイルス / 感染性クローン / 組換えウイルス / 弱毒生ワクチン / 外部寄生虫 |
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| Outline of Final Research Achievements |
The poultry red mite (PRM), is one of the major hematophagous ectoparasites of poultry farming, and the decreased productivity due to blood-sucking by PRMs is a severe problem for the poultry industry. The use of acaricides often leads to the spread of acaricide-resistant PRMs. Therefore, alternative strategies are required to reduce economic losses in poultry farming. Here, we focus on preventive methods such as vaccination to control PRMs, and we have already identified several vaccine candidates. In order to prepare an easy-to-use anti-PRM vaccine, the recombinant Marek's disease (MD) virus (rMDV) expressing a vaccine antigen was reconstituted. Reconstituted rMDV showed no significant differences in the growth kinetics compared to the parental strain, and the vaccine antigen was expressed in the infected cells. Thus, the rMDV live vaccine expressing a vaccine antigen against PRMs may be applicable as a candidate dual vaccine that provides protection against both PRMs and MD.
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Report
(3 results)
Research Products
(39 results)
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[Journal Article] Prostaglandin E2 induction suppresses the Th1 immune responses in cattle with Johne's disease.2018
Author(s)
Sajiki Y, Konnai S, Okagawa T, Nishimori A, Maekawa N, Goto S, Ikebuchi R, Nagata R, Kawaji S, Kagawa Y, Yamada S, Kato Y, Nakajima C, Suzuki Y, Murata S, Mori Y, Ohashi K.
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Journal Title
Infect. Immun.
Volume: in press
Issue: 5
DOI
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Peer Reviewed
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[Journal Article] In vitro and in vivo antivirus activity of an anti-programmed death-ligand 1 (PD-L1) rat-bovine chimeric antibody against bovine leukemia virus infection.2017
Author(s)
Nishimori A, Konnai S, Okagawa T, Maekawa N, Ikebuchi R, Goto S, Sajiki Y, Suzuki Y, Kohara J, Ogasawara S, Kato Y, Murata S, Ohashi K.
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Journal Title
PLoS One
Volume: 印刷中
Issue: 4
Pages: e0174916-e0174916
DOI
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Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Anti-Bovine Programmed Death-1 Rat-Bovine Chimeric Antibody for Immunotherapy of Bovine Leukemia Virus Infection in Cattle.2017
Author(s)
Okagawa, T., Konnai, S., Nishimori, A., Maekawa, N., Ikebuchi, R., Goto, S., Nakajima, C., Kohara, J., Ogasawara, S., Kato, Y., Suzuki, Y., Murata, S. and Ohashi, K.
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Journal Title
Front Immunol.
Volume: 8
Pages: 650-650
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Identification of an atypical enzootic bovine leukosis in Japan by using a novel classification of bovine leukemia based on immunophenotypic analysis.2017
Author(s)
Nishimori, A., Konnai, S., Okagawa, T., Maekawa, N., Goto, S., Ikebuchi, R., Nakahara, A., Chiba, Y., Ikeda, M., Murata, S. and Ohashi, K.
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Journal Title
Clin. Vaccine Immunol.
Volume: CVI.00067-17.
Issue: 9
DOI
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Peer Reviewed / Open Access
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[Journal Article] Intrauterine infection with bovine leukemia virus in pregnant dam with high viral load2017
Author(s)
Sajiki Y, Konnai S, Nishimori A, Okagawa T, Maekawa N, Goto S, Nagano M, Kohara J, Kitano N, Takahashi T, Tajima M, Mekata H, Horii Y, Murata S, Ohashi K.
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Journal Title
Journal of Veterinary Medical Science
Volume: 79
Issue: 12
Pages: 2036-2039
DOI
NAID
ISSN
0916-7250, 1347-7439
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Peer Reviewed / Open Access
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[Presentation] 牛マイコプラズマ感染症における免疫抑制機序の解明2017
Author(s)
後藤 伸也, 今内 覚, 岡川 朋弘, 西森 朝美, 前川 直也, 樋口 豪紀, 小岩 政照, 田島 誉士, 小原 潤子, 加藤 幸成, 鈴木 定彦, 村田 史郎, 大橋 和彦
Organizer
第160回日本獣医学会学術集会
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