Spatial-Temporal profile of ultrasound-mediated cisplatin delivery

• Project/Area Number: 16K18799
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Veterinary medical science
• Research Institution: Hokkaido University
• Principal Investigator: SASAKI Noboru 北海道大学, 獣医学研究科, 助教 (00754532)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 超音波治療 / ドラッグデリバリー / 細胞内薬剤濃度 / 非侵襲 / 膀胱癌 / 細胞内濃度 / 抗がん剤 / 3次元培養

Outline of Final Research Achievements

Ultrasound-triggered microbubble cavitation is a promising tool for drug and gene delivery in cancer therapy. We evaluated the temporal and spatial profile of cisplatin delivery by ultrasound-triggered microbubble cavitation. We used a bladder cancer mimicking 3D culture model for this study. Single treatment of ultrasound-triggered microbubble cavitation increased an intracellular concentration of cisplatin at the thickness of 100 μm. The duration of enhanced delivery was 1 hr. Based on the distance and duration of delivery, we further tested the feasibility of repetition of the treatment. Triple treatment increased the effective distance by 2-fold. Our results clearly showed spatial and temporal profile of delivery by ultrasound-triggered microbubble cavitation in a tumor-mimicking structure. The results of this study suggest that ultrasound-triggered microbubble cavitation can be repeatable and is promising for cancer treatment.

Research Products

• [Journal Article] Spatial and temporal profile of cisplatin delivery by ultrasound-assisted intravesical chemotherapy in a bladder cancer model (2017)
  • Author(s): Sasaki Noboru、Ishi Kazuhiro、Kudo Nobuki、Nakayama Shouta M. M.、Nakamura Kensuke、Morishita Keitaro、Ohta Hiroshi、Ishizuka Mayumi、Takiguchi Mitsuyoshi
  • Journal Title: PLOS ONE Volume: 12 Issue: 11 Pages: e0188093-e0188093
  • DOI: 10.1371/journal.pone.0188093
  • Peer Reviewed / Open Access
• [Journal Article] ソノポレーションを用いた膀胱内注入療法の開発 (2017)
  • Author(s): 佐々木東、工藤信樹、中山翔太、滝口満喜
  • Journal Title: 超音波TECHNO Volume: 29 Pages: 27-30
• [Presentation] Spatial and Temporal Window for cisplatin delivery in a 3D culture model (2017)
  • Author(s): Noboru Sasaki, Nobuki Kudo, Shouta MM Nakayama, Kensuke Nakamura, Keitaro Morishita, Hiroshi Ohta, Mitsuyoshi Takiguchi
  • Organizer: The 22nd European Symposium on Ultrasound Contrast Imaging
  • Place of Presentation: Hilton Hotel, Rotterdam, The Netherlands
  • Year and Date: 2017-01-19
  • Int'l Joint Research
• [Presentation] 超音波と膀胱内注入療法を組み合わせた膀胱癌治療法の開発 (2017)
  • Author(s): 佐々木東、中山翔太、森下啓太郎、中村健介、大田寛、水川菜月、池中良徳、石塚真由美、滝口満喜
  • Organizer: 第160回日本獣医学会学術集会
• [Presentation] Low intensity pulsed ultrasound and microbubbles increase cellular cisplatin and enhance cisplatin effect in a bladder cancer culture model (2016)
  • Author(s): Noburu Sasaki, Mitsuyoshi Takiguchi
  • Organizer: 5th International Symposium on Focused Ultrasound
  • Place of Presentation: Bethesda North Marriot Hotel & Conference Center, Bethesda, MD, USA
  • Year and Date: 2016-08-28
  • Int'l Joint Research
• [Presentation] ドラッグデリバリーの効果が及ぶ距離 (2016)
  • Author(s): 佐々木東、工藤信樹、中村健介、森下啓太郎、大田寛、滝口満喜
  • Organizer: 日本超音波医学会第89回学術集会
  • Place of Presentation: 京都国際会議場、京都市
  • Year and Date: 2016-05-27
• [Presentation] 本当にソノポレーションか？ (2016)
  • Author(s): 佐々木東、工藤信樹、中山翔太、滝口満喜
  • Organizer: 日本超音波医学会平成28年度第1回超音波分子診断治療研究会
  • Place of Presentation: 北海道大学大学院情報科学研究科、札幌市
• [Presentation] 超音波とマイクロバブルによるデリバリーの時間的制約 (2016)
  • Author(s): 佐々木東、石井和浩、工藤信樹、滝口満喜
  • Organizer: 第15回日本超音波治療研究会
  • Place of Presentation: 東京女子医科大学TWIns、東京都新宿区