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Study for the relationship between hypoxia and PG-resistance in bovine early corpus luteum -roles of BNIP3 in early corpus luteum-

Research Project

Project/Area Number 16K18803
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Veterinary medical science
Research InstitutionTottori University

Principal Investigator

NISHIMURA Ryo  鳥取大学, 農学部, 助教 (20704901)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2019: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywordsウシ / 黄体 / 低酸素環境 / 黄体形成 / 黄体退行 / TGFb / progesterone / apoptosis / GLUT1 / glucose / アポトーシス / betaglycan / 牛 / 初期黄体 / PG抵抗性 / HIF1 / 臨床繁殖・産科 / 卵巣 / BNIP3
Outline of Final Research Achievements

This project has revealed as follows:
1)Hypoxia induces BNIP3 and GLUT1 expressions in bovine early corpus luteum (CL), resulting in supporting bovine luteal formation (J Vet Med Sci 2017; 79: 1878-1883., J Vet Med Sci 2018; 80: 368-374). 2)Bovine early luteal cells maintain production of progesterone (P4) under hypoxic conditions during luteal formation (J Reprod Dev 2019; 65: 67-72). 3)Decrease of glucose supply via GLUT1 is related to bovine luteal cell apoptosis. 4)TGFβ inhibits P4 production and facilitates apoptosis in bovine luteal cells.

Academic Significance and Societal Importance of the Research Achievements

本研究では哺乳動物の卵巣における低酸素環境の生理的役割について研究を展開し、特にウシの黄体形成機構に着目して研究を進めた。特に、排卵後数日の初期黄体内における低酸素環境に誘導される因子の特定、およびそれらの因子と黄体のプロジェステロン産生の関係について明らかにした。本研究ではウシを対象としたが、その成果は哺乳動物全体としても新しいものであり、ヒトを含めた動物種で同様の解析を進める際の重要な参考資料となる。家畜であるウシの卵巣の生理機構を解明することは、その機構を利用した繁殖制御に応用でき、他の動物種も含めた新しい動物生産技術開発の基礎データとなる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (15 results)

All 2020 2019 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 5 results,  Open Access: 5 results) Presentation (9 results) (of which Int'l Joint Research: 2 results,  Invited: 3 results) Book (1 results)

  • [Journal Article] Multiple roles of hypoxia in bovine corpus luteum.2020

    • Author(s)
      Nishimura R, Okuda K.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 66 Issue: 4 Pages: 307-310

    • DOI

      10.1262/jrd.2020-018

    • NAID

      130007888719

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Effect of hypoxia on progesterone production by cultured bovine early and mid luteal cells2019

    • Author(s)
      Hasegawa H, Nishimura R, Yamashita M, Yamaguchi T, Hishinuma M, Okuda K.
    • Journal Title

      Journal of Reproduction and Development

      Volume: 65 Issue: 1 Pages: 67-72

    • DOI

      10.1262/jrd.2018-061

    • NAID

      130007591420

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] BNIP3 expression in bovine follicle and corpus luteum2018

    • Author(s)
      NISHIMURA Ryo、OKUDA Kiyoshi、GUNJI Yosuke、KHALID Ahmed Magzoub、YAMANO Yoshiaki、YAMASHITA Yasuhisa、HISHINUMA Mitsugu
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 80 Issue: 2 Pages: 368-374

    • DOI

      10.1292/jvms.17-0267

    • NAID

      130006410764

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Hypoxia increases glucose transporter 1 expression in bovine corpus luteum at the early luteal stage2017

    • Author(s)
      NISHIMURA Ryo、HASEGAWA Hiroki、YAMASHITA Masamichi、ITO Norihiko、OKAMOTO Yoshiharu、TAKEUCHI Takashi、KUBO Tomoaki、IGA Kosuke、KIMURA Koji、HISHINUMA Mitsugu、OKUDA Kiyoshi
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 79 Issue: 11 Pages: 1878-1883

    • DOI

      10.1292/jvms.17-0284

    • NAID

      130006206584

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Hypoxia-inducible factor 1 mediates hypoxia-enhanced synthesis of progesterone during luteinization of granulosa cells2017

    • Author(s)
      Shin Yoshioka, Ryo Nishimura, Yuki Yamamoto, Koji Kimura, Kiyoshi Okuda
    • Journal Title

      Journal of Reproduction and Development

      Volume: 63 Issue: 1 Pages: 75-85

    • DOI

      10.1262/jrd.2016-068

    • NAID

      130005332285

    • ISSN
      0916-8818, 1348-4400
    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Presentation] Glucose 供給の低下はウシ黄体の構造的退行に関与する2019

    • Author(s)
      西村 亮,神保 葵,長谷川啓喜,山下真路,山口武視,菱沼 貢,奥田 潔
    • Organizer
      日本繁殖生物学会
    • Related Report
      2019 Annual Research Report
  • [Presentation] Multiple roles of hypoxia in bovine corpus luteum function2019

    • Author(s)
      Ryo NISHIMURA, Kiyoshi OKUDA
    • Organizer
      JSPS & PAS Japanese-Polish Joint Seminar “Cutting-edge Reproductive Physiology-Key process for birth of a new life”
    • Related Report
      2019 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] ウシ黄体における骨形成蛋白質結合蛋白質の役割.2018

    • Author(s)
      西村 亮,長谷川啓喜,山下真路,伊藤典彦,岡本芳晴,木村康二,菱沼 貢,奥田 潔
    • Organizer
      第 161 回日本獣医学会
    • Related Report
      2018 Research-status Report
  • [Presentation] ウシ黄体における betaglycan の役割とその発現調節2018

    • Author(s)
      長谷川啓喜、山下真路、伊藤典彦、岡本芳晴、窪 友瑛、伊賀浩輔、木村康二、菱沼 貢、奥田 潔、西村 亮
    • Organizer
      第 124 回日本畜産学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ウシ初期黄体における低酸素環境の glucose transporter 1 (GLUT1) 発現を介した機能亢進2017

    • Author(s)
      西村 亮、長谷川啓喜、山下真路、伊藤典彦、岡本芳晴、窪 友瑛、伊賀浩輔、木村康二、菱沼 貢、奥田 潔
    • Organizer
      第 160 回日本獣医学会学術集会
    • Related Report
      2017 Research-status Report
  • [Presentation] Possible role of glucose transporter-1 in progesterone synthesis in bovine corpus luteum.2017

    • Author(s)
      Hiroki Hasegawa, Mitsugu Hishinuma, Masamichi Yamashita, Norihiko Ito, Yoshiharu Okamoto, Tomoaki Kubo, Kosuke Iga, Koji Kimura, Kiyoshi Okuda and Ryo Nishimura.
    • Organizer
      4th World Congress of Reproductive Biology (WCRB 2017)
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] ウシ初期および中期黄体細胞のプロジェステロン (P4) 分泌に及ぼす低酸素環境の影響2017

    • Author(s)
      長谷川啓喜・西村 亮・菱沼 貢・山下真路・伊藤典彦・岡本芳晴・窪 友瑛・伊賀浩輔・木村康二・奥田 潔
    • Organizer
      日本畜産学会第122回大会
    • Place of Presentation
      兵庫県神戸市神戸大学鶴甲第1キャンパス
    • Related Report
      2016 Research-status Report
  • [Presentation] 黄体の消長と低酸素環境2017

    • Author(s)
      西村 亮
    • Organizer
      北海道受精卵移殖研究会シンポジウム
    • Place of Presentation
      北海道札幌市北海道大学
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] ウシ黄体の消長と酸素濃度の関係2017

    • Author(s)
      西村 亮
    • Organizer
      岡山県農業共済組合連合会平成28年度家畜診療技術者講習会
    • Place of Presentation
      岡山県岡山市岡山県農業共済会館
    • Related Report
      2016 Research-status Report
    • Invited
  • [Book] The Life Cycle Of The Corpus Luteum2016

    • Author(s)
      Okuda K, Nishimura R (Meidan R. eds.)
    • Total Pages
      283
    • Publisher
      Springer press
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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