Establishment of an in vitro oocyte production system toward understanding a totipotency

• Project/Area Number: 16K18816
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Kyushu University
• Principal Investigator: HAMAZAKI NOBUHIKO 九州大学, 医学研究院, 助教 (10757008)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: in vitro 卵母細胞分化誘導 / トランスクリプトーム / RNA-seq / ES・iPS細胞 / 全能性 / 生殖細胞 / in vitro卵母細胞誘導 / ES細胞 / 卵子 / 試験管内 / 分化 / in vitro oogenesis / Oocyte differentiation / Totipotency / Developmental capacity / Transcriptome / Oogenesis / Transcriptional network / in vitro 配偶子形成 / transcriptome

Outline of Final Research Achievements

In multicellular organisms, oocytes possess an incredible ability to complete the development from a single cell. The ability is supported in part by unique features of oocytes such as a large cell size, specialized organelles and stored maternal factors. These features must be orchestrated by oocyte-specific transcriptional networks. However, due to the lack of an experimental system that enables the evaluation of multiple gene functions in oogenesis, there is no comprehensive and systematic study to identify the transcriptional networks. Hence, we recently developed an in vitro oocyte differentiation system from mouse pluripotent stem cells. This system has a great potential to clarify the basis of totipotency in oocytes.

Research Products

• [Journal Article] Reconstitution in vitro of the entire cycle of the mouse female germ line. (2016)
  • Author(s): Hikabe O, Hamazaki N, Nagamatsu G, Obata Y, Hirao Y, Hamada N, Shimamoto S, Imamura T, Nakashima K, Saitou M, Hayashi K
  • Journal Title: Nature Volume: 539 Issue: 7628 Pages: 299-303
  • DOI: 10.1038/nature20104
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] Transcriptional regulatory networks controlling the oocyte identity (2017)
  • Author(s): Nobuhiko Hamazaki, So Shimamoto, Orie Hikabe, Norio Hamada, Katsuhiko Hayashi
  • Organizer: CDB symposium
  • Int'l Joint Research
• [Presentation] Uncovering the gene-networks regulating oogenesis (2017)
  • Author(s): Nobuhiko Hamazaki, So Shimamoto, Orie Hikabe, Norio Hamada, Katsuhiko Hayashi
  • Organizer: 4th World Congress of Reproductive Biology 2017
  • Int'l Joint Research
• [Presentation] Uncovering the gene-networks shaping an oocyte (2017)
  • Author(s): Nobuhiko Hamazaki, So Shimamoto, Orie Hikabe, Yohei Nishimura, Norio Hamada, Katsuhiko Hayashi
  • Organizer: Gordon Research Conference
  • Int'l Joint Research
• [Remarks] 成体マウスiPS細胞から体外培養で卵子の作製に世界で初めて成功 ～不妊原因の究明や治療法の開発に光～
  • URL: http://www.kyushu-u.ac.jp/ja/researches/view/53