| Project/Area Number |
16K18816
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Integrative animal science
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | in vitro 卵母細胞分化誘導 / トランスクリプトーム / RNA-seq / ES・iPS細胞 / 全能性 / 生殖細胞 / in vitro卵母細胞誘導 / ES細胞 / 卵子 / 試験管内 / 分化 / in vitro oogenesis / Oocyte differentiation / Totipotency / Developmental capacity / Transcriptome / Oogenesis / Transcriptional network / in vitro 配偶子形成 / transcriptome |
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| Outline of Final Research Achievements |
In multicellular organisms, oocytes possess an incredible ability to complete the development from a single cell. The ability is supported in part by unique features of oocytes such as a large cell size, specialized organelles and stored maternal factors. These features must be orchestrated by oocyte-specific transcriptional networks. However, due to the lack of an experimental system that enables the evaluation of multiple gene functions in oogenesis, there is no comprehensive and systematic study to identify the transcriptional networks. Hence, we recently developed an in vitro oocyte differentiation system from mouse pluripotent stem cells. This system has a great potential to clarify the basis of totipotency in oocytes.
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