Analysis of neural crest-derived cells in pituitary development

• Project/Area Number: 16K18818
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Integrative animal science
• Research Institution: Jikei University School of Medicine (2017-2018); Meiji University (2016)
• Principal Investigator: YOSHIDA SAISHU 東京慈恵会医科大学, 医学部, 助教 (40772744)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
• Keywords: 下垂体 / 神経堤細胞 / 幹細胞 / 発生 / 分化 / S100b / 幹細胞ニッチ / 腫瘍化

Outline of Final Research Achievements

The anterior lobe of the pituitary gland is a key endocrine organ which is believed to derive from the ectoderm. In this study, we attempt to clarify the role of neural crest cells (NCCs) in pituitary development using P0-Cre/EGFP reporter mice. Fate tracing of NCCs demonstrated that NCCs differentiate into all types of pituitary hormone-producing cells as well as pituitary stem/progeny cells. These results showed that NCCs contribute to pituitary organogenesis.

Academic Significance and Societal Importance of the Research Achievements

本研究から、これまで外胚葉由来と考えられてきた下垂体前葉に関して、神経堤細胞がその起源の一部を構成することを見出した。同一のホルモン産生細胞種の中に、異なる起源の細胞が存在することが、下垂体の機能維持や腫瘍化に対して、どのような意義を有するのかが今後の重要な課題である。こうした知見は、脳腫瘍の約17%を占める下垂体腫瘍の発生機序に対する新知見に繋がると期待される。

Research Products

• [Journal Article] Characteristic Localization of Neuronatin in Rat Testis, Hair Follicle, Tongue, and Pancreas. (2019)
  • Author(s): Kanno N, Yoshida S, Kato T, Kato Y
  • Journal Title: The Journal of Histochemistry & Cytochemistry Volume: in press Issue: 7 Pages: 495-509
  • DOI: 10.1369/0022155419836433
  • Peer Reviewed / Open Access
• [Journal Article] Forced expression of DYRK2 exerts anti-tumor effects via apoptotic induction in liver cancer. (2019)
  • Author(s): Yokoyama-Mashima S, Yogosawa S, Kanegae Y, Hirooka S, Yoshida S, Horiuchi T, Ohashi T, Yanaga K, Saruta M, Oikawa T, Yoshida K.
  • Journal Title: Cancer Lett Volume: 451 Pages: 100-109
  • DOI: 10.1016/j.canlet.2019.02.046
  • Peer Reviewed / Open Access
• [Journal Article] Data on localization of coxsackievirus and adenovirus receptor (CAR) in prenatal and adult rat olfactory, intestine, pancreas, liver, ovary, and testis (2019)
  • Author(s): Chen M, Yoshida S, Kato T, Kato Y
  • Journal Title: Data in Brief Volume: 24 Pages: 103797-103797
  • DOI: 10.1016/j.dib.2019.103797
  • Peer Reviewed / Open Access
• [Journal Article] Identification of TGFβ-induced proteins in non-endocrine mouse pituitary cell line TtT/GF by SILAC-assisted quantitative mass spectrometry (2019)
  • Author(s): Tsukada Takehiro、Isowa Yukinobu、Kito Keiji、Yoshida Saishu、Toneri Seina、Horiguchi Kotaro、Fujiwara Ken、Yashiro Takashi、Kato Takako、Kato Yukio
  • Journal Title: Cell and Tissue Research Volume: 376 Issue: 2 Pages: 281-293
  • DOI: 10.1007/s00441-018-02989-2
  • Peer Reviewed
• [Journal Article] Localization of SOX2-positive stem/progenitor cells in the anterior lobe of the common marmoset (<i>Callithrix jacchus</i>) pituitary (2018)
  • Author(s): YOSHIDA Saishu、FUJIWARA Ken、INOUE Takashi、SASAKI Erika、KAMETANI Yoshie、TAKEKOSHI Susumu、INOSHITA Naoko、KATO Takako、KATO Yukio
  • Journal Title: Journal of Reproduction and Development Volume: 64 Issue: 5 Pages: 417-422
  • DOI: 10.1262/jrd.2018-043
  • NAID: 130007497306
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] 下垂体の機能維持と幹・前駆細胞: Pituitary stem/progenitor cells for pituitary homeostasis (2018)
  • Author(s): 吉田彩舟
  • Journal Title: 月刊 「アグリバイオ」 Volume: 2 Pages: 84-87
• [Journal Article] 下垂体組織幹・前駆細胞の特性とその起源の多様: Characteristics and plural origins of stem/progenitor cells in the pituitary gland (2018)
  • Author(s): 吉田彩舟
  • Journal Title: 月刊 「細胞」 Volume: 50 Pages: 47-50
• [Journal Article] Retinoic acid signalling is a candidate regulator of the expression of pituitary-specific transcription factor Prop1 in the developing rodent pituitary (2018)
  • Author(s): Yoshida S.、Fujiwara K.、Nishihara H.、Kato T.、Yashiro T.、Kato Y.
  • Journal Title: Journal of Neuroendocrinology Volume: 30 Issue: 3
  • DOI: 10.1111/jne.12570
  • Peer Reviewed / Open Access
• [Journal Article] TGFβ signaling reinforces pericyte properties of the non-endocrine mouse pituitary cell line TtT/GF. (2018)
  • Author(s): Tsukada T, Yoshida S, Kito K, Fujiwara K, Yako H, Horiguchi K, Isowa Y, Yashiro T, Kato T, Kato Y.
  • Journal Title: Cell Tissue Res. Volume: 371 Issue: 2 Pages: 339-350
  • DOI: 10.1007/s00441-017-2758-x
  • Peer Reviewed
• [Journal Article] Isolation and characterisation of CD9-positive pituitary adult stem/progenitor cells in rats (2018)
  • Author(s): Horiguchi Kotaro、Fujiwara Ken、Yoshida Saishu、Nakakura Takashi、Arae Ken、Tsukada Takehiro、Hasegawa Rumi、Takigami Shu、Ohsako Shunji、Yashiro Takashi、Kato Takako、Kato Yukio
  • Journal Title: Scientific Reports Volume: 8 Issue: 1 Pages: 1-15
  • DOI: 10.1038/s41598-018-23923-0
  • Peer Reviewed / Open Access
• [Journal Article] Differentiation capacities of PS-clusters, adult pituitary stem/progenitor cell clusters located in the parenchymal-niche, of the rat anterior lobe (2018)
  • Author(s): S. Yoshida, N. Nishimura, H. Yurino, M. Kobayashi, K. Horiguchi, K. Yano, SI. Hashimoto, T. Kato, Y. Kato
  • Journal Title: PLOS ONE Volume: 13 Issue: 4 Pages: e0196029-e0196029
  • DOI: 10.1371/journal.pone.0196029
  • Peer Reviewed / Open Access
• [Journal Article] Cell type-specific localization of Ephs pairing with ephrin-B2 in the rat postnatal pituitary gland. (2017)
  • Author(s): Yoshida S, Kato T, Kanno N, Nishimura N, Nishihara H, Horiguchi K, Kato Y.
  • Journal Title: Cell Tissue Res. Volume: 370 Issue: 1 Pages: 99-112
  • DOI: 10.1007/s00441-017-2646-4
  • Peer Reviewed
• [Journal Article] Construction and expression of vectors encoding biologically active rodent gonadotropins (2017)
  • Author(s): A. Ohta, Y. Tsunoda, Y. Tamura, K. Iino, N. Nishimura, H. Nishihara, H. Takanashi, S. Yoshida, T. Kato, Y. Kato.
  • Journal Title: Journal of Reproduction and Development Volume: 63 Issue: 6 Pages: 605-609
  • DOI: 10.1262/jrd.2017-091
  • NAID: 130006250258
  • ISSN: 0916-8818, 1348-4400
  • Peer Reviewed / Open Access
• [Journal Article] SOX10-positive cells emerge in the rat pituitary gland during late embryogenesis and start to express S100B (2017)
  • Author(s): H. Ueharu, S. Yoshida, N. Kanno, K. Horiguchi, N. Nishimura, T. Kato, Y. Kato
  • Journal Title: Cell and Tissue Res. Volume: 372 Issue: 1 Pages: 77-90
  • DOI: 10.1007/s00441-017-2724-7
  • Peer Reviewed
• [Journal Article] Gene tracing analysis reveals the contribution of neural crest-derived cells in pituitary development (2016)
  • Author(s): Ueharu H, Yoshida S, Kikkawa T, Kanno N, Higuchi M, Kato T, Osumi N, Kato Y
  • Journal Title: Journal of Anatomy Volume: 230 Issue: 3 Pages: 373-380
  • DOI: 10.1111/joa.12572
  • Peer Reviewed / Open Access
• [Presentation] レチノイン酸シグナルは下垂体特異的転写因子Prop1の発現を制御している (2018)
  • Author(s): 吉田彩舟、藤原研、西原大翔、堀口幸太郎、加藤たか子、屋代隆、加藤幸雄
  • Organizer: 第45回 日本神経内分泌学会学術集会
• [Presentation] 下垂体発生過程における転写因子Prop1のレチノイン酸シグナルによる発現制御解析 (2018)
  • Author(s): 吉田彩舟、藤原研、西原大翔、堀口幸太郎、加藤たか子、屋代隆、加藤幸雄
  • Organizer: 第111回 日本繁殖生物学会大会
• [Presentation] 下垂体特異的転写因子Prop1の発現はレチノイン酸による制御を受ける (2018)
  • Author(s): 吉田彩舟、藤原研、西原大翔、堀口幸太郎、加藤たか子、屋代隆、加藤幸雄
  • Organizer: 第30回 日本下垂体研究会学術集会
• [Presentation] 下垂体の組織幹・前駆細胞の多様性 (2018)
  • Author(s): 吉田彩舟
  • Organizer: 第44回 日本神経内分泌学会学術集会
  • Invited
• [Presentation] 成体下垂体前葉から単離した幹細胞ニッチを用いた分化誘導と制御因子の探索 (2017)
  • Author(s): 吉田彩舟、小林正明、百合野秀朗、西原大翔、上春浩貴、菅野尚子、矢野健太郎、橋本真一、屋代隆、加藤幸雄.
  • Organizer: 第90回日本内分泌学会学術総会
• [Presentation] Analysis of the differentiation capacity of adult stem/progenitor cells in the parenchymal-niche of the rodent pituitary gland (2017)
  • Author(s): S. Yoshida, H. Yurino, M. Kabayashi, N. Nishinura, N. Kanno, K.Yano, SI. Hashimoto, T. Kato, Y. Kato
  • Organizer: International Society for Stem Cell Research 2017 Annual meeting
  • Int'l Joint Research
• [Presentation] ニッチから単離した下垂体幹細胞塊の分化能の解析 (2017)
  • Author(s): 吉田彩舟、加藤たか子、加藤幸雄
  • Organizer: 第35回内分泌代謝学サマーセミナー
• [Presentation] 下垂体前葉から単離したSOX2陽性細胞塊の性質解析 (2017)
  • Author(s): 吉田彩舟、百合野秀朗、小林正明、田村祐介、菅野鉱、矢野健太郎、橋本真一、加藤たか子、加藤幸雄
  • Organizer: 第30回日本下垂体研究会学術集会
• [Presentation] 成体下垂体における組織幹細胞の解析 (2017)
  • Author(s): 吉田彩舟、加藤たか子、加藤幸雄
  • Organizer: 日本動物学会第88回大会
  • Invited