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Elucidation of mechanisms related to actin cytoskeleton dynamics of dedifferentiation and acquisition of multipotency in mature adipocytes

Research Project

Project/Area Number 16K18822
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Integrative animal science
Research InstitutionNihon University

Principal Investigator

OKI Yoshinao  日本大学, 生物資源科学部, 助教 (70525667)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2018: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Keywords脂肪細胞 / アクチン細胞骨格 / 脱分化 / DFAT / アクチン / 細胞形態 / 幹細胞 / 細胞骨格
Outline of Final Research Achievements

During the mature adipocyte dedifferentiation, loss of lipid droplets occurs with remodeling of actin cytoskeleton. Transcriptome analysis identified MKL1 also known as actin-binding transcriptional coactivator, as upregulated gene after dedifferentiation of mature adipocyte. Formation of actin stress fiber in dedifferentiation of mature adipocyte resulted in the nuclear accumulation of MKL1. Furthermore, the formation of actin stress fibres and the nuclear translocation of MKL1 was accompanied by a marked decrease in the expression of PPARγ. These results suggest that regulation of MKL1 by actin cytoskeleton dynamics is a critical part of dedifferentiation of mature adipocytes.

Academic Significance and Societal Importance of the Research Achievements

本研究は極めて簡便な方法によって体細胞から脱分化および多能性獲得した細胞を大量に取得できる独自の培養系を用いて、その仕組みを解明するものである。幹細胞のみが具備すると考えられている多能性について、脱分化細胞がなぜその能力を有するのかを明らかにすることは、一個の受精卵から多種多様な機能をもつ細胞(分化細胞)が生ずる仕組みの解明に大きく貢献すると考えられる。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (11 results)

All 2019 2018 2017 2016 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (7 results) Remarks (3 results)

  • [Journal Article] Effect of collagenase concentration for isolating small adipocytes from human buccal fat pad.2017

    • Author(s)
      Tsurumachi N, Akita D, Kano K, Matsumoto T, Toriumi T, Kazama, T, Oki, Y, Saito Y, Tonogi M, Shimizu N
    • Journal Title

      Journal of Oral Science

      Volume: 印刷中

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 脱分化脂肪細胞に由来する肝細胞は中心静脈周辺領域の肝細胞の特徴をもつ2019

    • Author(s)
      萩原 玲子, 沖 嘉尚, 加野 浩一郎
    • Organizer
      日本畜産学会第125回大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Identification of master regulator genes for hepatocyte differentiation in de- differentiated fat (DFAT) cells2018

    • Author(s)
      Reiko Hagiwara, Yoshinao Oki, Koichiro Kano
    • Organizer
      第70回日本細胞生物学会 第51回日本発生生物学会 合同大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 公共データベースを利用した脱分化脂肪細胞における肝細胞分化制御因子の探索2018

    • Author(s)
      萩原 玲子, 沖 嘉尚, 加野 浩一郎
    • Organizer
      第70回日本生物工学会大会
    • Related Report
      2018 Annual Research Report
  • [Presentation] 成熟脂肪細胞由来の DFAT はセロトニンおよびグルタミン酸作動性神経細胞様細胞へと分化する2017

    • Author(s)
      沖嘉尚、渡辺真平、加野浩一郎
    • Organizer
      日本畜産学会第122回大会
    • Place of Presentation
      神戸大学鶴甲第1キャンパス(兵庫県神戸市)
    • Year and Date
      2017-03-28
    • Related Report
      2016 Research-status Report
  • [Presentation] 脱分化脂肪細胞における肝細胞分化制御因子の探索2017

    • Author(s)
      萩原玲子・伊林志穂・沖嘉尚・加野浩一郎
    • Organizer
      日本畜産学会124回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] Identification of the master regulator gene for hepatocyte differentiation in de-differentiated fat (DFAT) cells2017

    • Author(s)
      Reiko Hagiwara, Shiho Ibayashi, Yoshinao Oki, Koichiro Kano
    • Organizer
      Consortium of Biological Sciences 2017
    • Related Report
      2017 Research-status Report
  • [Presentation] 成熟脂肪細胞に由来する脱分化脂肪細胞のセロトニン作動性神経細胞様細胞への分化2016

    • Author(s)
      渡辺真平、沖嘉尚、加野浩一郎
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      パシフィコ横浜(神奈川県横浜市)
    • Year and Date
      2016-12-01
    • Related Report
      2016 Research-status Report
  • [Remarks] 日本大学動物生体機構学研究室ホームページ

    • URL

      http://hp.brs.nihon-u.ac.jp/~seitaikiko/k/TOP.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 日本大学生物資源科学部研究ニュース

    • URL

      http://www.brs.nihon-u.ac.jp/news01/18676.html

    • Related Report
      2017 Research-status Report
  • [Remarks] 日本大学生物資源科学部応用生物科学科動物生体機構学研究室ホームページ

    • URL

      http://hp.brs.nihon-u.ac.jp/~seitaikiko/k/TOP.html

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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