Study of genes related to blood feeding and digestion for anopheline mosquito control
| Project/Area Number |
16K18824
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Insect science
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| Research Institution | Jichi Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2020-03-31
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| Project Status |
Completed (Fiscal Year 2019)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
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| Keywords | 吸血 / 発生・分化 / ゲノム編集 / 害虫防除 / ベクターバイオロジー / 性分化 / 昆虫 / ベクター制御 |
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| Outline of Final Research Achievements |
In order to elucidate genes important for blood feeding and digestion in anopheline mosquitoes, gene expression of the midgut and salivary glands of female that exhibited the dysfunction in blood digestion due to knockout of the sex differentiation gene was analyzed. In the midgut, comprehensive comparative analyses were performed between this females and wild-type females, and genes with the different expression levels were clarified. Among these genes, the aminopeptidase gene, which is predicted to be a digestive enzyme based on the amino structure and homology searches, was subjected to knockout analysis by CRISPR/Cas9 and revealed to be involved in female fertility.
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| Academic Significance and Societal Importance of the Research Achievements |
本研究では血液消化不良をおこすハマダラカ系統を用いることで、血液消化・吸収に関与する可能性のある遺伝子群を明らかにするとともに、これらの遺伝子発現の制御に性分化遺伝子が直接的あるいは間接的に関与することを明らかした。ハマダラカにおいて中腸や唾液腺は血液の消化・吸収や吸血行動に重要なため、これらの組織で発現する遺伝子を制御できれば、吸血不良や消化不良などによる妊性低下や致死により蚊の防除が期待出来る。本研究で明らかになった遺伝子群はマラリアベクター制御のための新しい標的になることが期待できる。
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Report
(5 results)
Research Products
(46 results)
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[Journal Article] A Viral-Vectored Multi-Stage Malaria Vaccine Regimen With Protective and Transmission-Blocking Efficacies2019
Author(s)
Yusuf Y, Yoshii T, Iyori M, Mizukami H, Fukumoto S, Yamamoto DS, Emran TB, Amelia F, Islam A, Syafira I, Yoshida S.
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Journal Title
Front Immunol
Volume: 10
Pages: 2412-2412
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Adeno-Associated Virus as an Effective Malaria Booster Vaccine Following Adenovirus Priming2019
Author(s)
Yusuf Y, Yoshii T, Iyori M, Yoshida K, Mizukami H, Fukumoto S, Yamamoto DS, Alam A, Emran TB, Amelia F, Islam A, Otsuka H, Takashima E, Tsuboi T, Yoshida S.
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Journal Title
Frontiers in Immunology
Volume: 10
Pages: 730-730
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Baculovirus-Induced Fast-Acting Innate Immunity Kills Liver-Stage Plasmodium.2018
Author(s)
Emran TB, Iyori M, Ono Y, Amelia F, Yusuf Y, Islam A, Alam A, Tamura M, Ogawa R, Matsuoka H, Yamaoto DS, Yoshida S.
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Journal Title
The Journal of Immunology
Volume: 201
Issue: 8
Pages: 2441-2441
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.2018
Author(s)
Yoshida K, Iyori M, Blagborough AM, Salman AM, Dulal P, Sala KA, Yamamoto DS, Khan SM, Janse CJ, Biswas S, Yoshii T, Yusuf Y, Tokoro M, Hill AVS, Yoshida S
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Journal Title
Scientific Reports
Volume: 8
Issue: 1
Pages: 3896-3896
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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