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Study of genes related to blood feeding and digestion for anopheline mosquito control

Research Project

Project/Area Number 16K18824
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionJichi Medical University

Principal Investigator

Daisuke Yamamoto  自治医科大学, 医学部, 講師 (90597189)

Project Period (FY) 2016-04-01 – 2020-03-31
Project Status Completed (Fiscal Year 2019)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Keywords吸血 / 発生・分化 / ゲノム編集 / 害虫防除 / ベクターバイオロジー / 性分化 / 昆虫 / ベクター制御
Outline of Final Research Achievements

In order to elucidate genes important for blood feeding and digestion in anopheline mosquitoes, gene expression of the midgut and salivary glands of female that exhibited the dysfunction in blood digestion due to knockout of the sex differentiation gene was analyzed. In the midgut, comprehensive comparative analyses were performed between this females and wild-type females, and genes with the different expression levels were clarified. Among these genes, the aminopeptidase gene, which is predicted to be a digestive enzyme based on the amino structure and homology searches, was subjected to knockout analysis by CRISPR/Cas9 and revealed to be involved in female fertility.

Academic Significance and Societal Importance of the Research Achievements

本研究では血液消化不良をおこすハマダラカ系統を用いることで、血液消化・吸収に関与する可能性のある遺伝子群を明らかにするとともに、これらの遺伝子発現の制御に性分化遺伝子が直接的あるいは間接的に関与することを明らかした。ハマダラカにおいて中腸や唾液腺は血液の消化・吸収や吸血行動に重要なため、これらの組織で発現する遺伝子を制御できれば、吸血不良や消化不良などによる妊性低下や致死により蚊の防除が期待出来る。本研究で明らかになった遺伝子群はマラリアベクター制御のための新しい標的になることが期待できる。

Report

(5 results)
  • 2019 Annual Research Report   Final Research Report ( PDF )
  • 2018 Research-status Report
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (46 results)

All 2020 2019 2018 2017 2016 Other

All Journal Article (17 results) (of which Int'l Joint Research: 8 results,  Peer Reviewed: 13 results,  Open Access: 13 results) Presentation (28 results) (of which Int'l Joint Research: 3 results,  Invited: 9 results) Remarks (1 results)

  • [Journal Article] Efforts to enhance safety measures for CRISPR/Cas-based gene drive technology in Japan2020

    • Author(s)
      Toshinori Tanaka, Nobukazu Tanaka, Yukio Nagano, Hirotaka Kanuka, Daisuke S Yamamoto, Natsuko Yamamoto, Eiji Nanba, Takumi Nishiuchi
    • Journal Title

      Journal of Environment and Safety

      Volume: 11 Issue: 2 Pages: 31-35

    • DOI

      10.11162/daikankyo.E19SC0801

    • NAID

      130007886580

    • ISSN
      1884-4375, 2186-3725
    • Year and Date
      2020-08-10
    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] A synthetic male-specific sterilization system using the mammalian pro-apoptotic factor in a malaria vector mosquito2019

    • Author(s)
      Yamamoto Daisuke S.、Sumitani Megumi、Kasashima Katsumi、Sezutsu Hideki、Matsuoka Hiroyuki、Kato Hirotomo
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 8160-8160

    • DOI

      10.1038/s41598-019-44480-0

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] PCR-RFLP analyses of Leishmania species causing cutaneous and mucocutaneous leishmaniasis revealed distribution of genetically complex strains with hybrid and mito-nuclear discordance in Ecuador2019

    • Author(s)
      Kato Hirotomo、Gomez Eduardo A.、Seki Chisato、Furumoto Hayato、Martini-Robles Luiggi、Muzzio Jenny、Calvopina Manuel、Velez Lenin、Kubo Makoto、Tabbabi Ahmed、Yamamoto Daisuke S.、Hashiguchi Yoshihisa
    • Journal Title

      PLOS Neglected Tropical Diseases

      Volume: 13 Issue: 5 Pages: e0007403-e0007403

    • DOI

      10.1371/journal.pntd.0007403

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Further insight into the geographic distribution of Leishmania species in Peru by cytochrome b and mannose phosphate isomerase gene analyses2019

    • Author(s)
      Kato H, Caceres AG, Seki C, Silupu Garcia CR, Holguin Mauricci C, Castro Martinez SC, Moreno Paico D, Castro Muniz JL et al.,
    • Journal Title

      PLOS Neglected Tropical Diseases

      Volume: 13 Issue: 6 Pages: e0007496-e0007496

    • DOI

      10.1371/journal.pntd.0007496

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A Viral-Vectored Multi-Stage Malaria Vaccine Regimen With Protective and Transmission-Blocking Efficacies2019

    • Author(s)
      Yusuf Y, Yoshii T, Iyori M, Mizukami H, Fukumoto S, Yamamoto DS, Emran TB, Amelia F, Islam A, Syafira I, Yoshida S.
    • Journal Title

      Front Immunol

      Volume: 10 Pages: 2412-2412

    • DOI

      10.3389/fimmu.2019.02412

    • Related Report
      2019 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ハマダラカにおける吸血及びマラリア媒介機構の研究2019

    • Author(s)
      山本大介
    • Journal Title

      衛生動物

      Volume: 70 Pages: 145-146

    • NAID

      130007733291

    • Related Report
      2019 Annual Research Report
    • Open Access
  • [Journal Article] Anopheline antiplatelet protein from mosquito saliva regulates blood feeding behavior.2019

    • Author(s)
      Islam A, Emran TB, Yamamoto DS, Iyori M, Amelia F, Yusuf Y, Yamaguchi R, Alam MS, Silveira H, Yoshida S.
    • Journal Title

      Scientific Reports

      Volume: 9 Issue: 1 Pages: 3129-3129

    • DOI

      10.1038/s41598-019-39960-2

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Adeno-Associated Virus as an Effective Malaria Booster Vaccine Following Adenovirus Priming2019

    • Author(s)
      Yusuf Y, Yoshii T, Iyori M, Yoshida K, Mizukami H, Fukumoto S, Yamamoto DS, Alam A, Emran TB, Amelia F, Islam A, Otsuka H, Takashima E, Tsuboi T, Yoshida S.
    • Journal Title

      Frontiers in Immunology

      Volume: 10 Pages: 730-730

    • DOI

      10.3389/fimmu.2019.00730

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Down‐selecting circumsporozoite protein‐based malaria vaccine: a comparison of malaria sporozoite challenge model2019

    • Author(s)
      Amelia F, Iyori M, Emran TB, Yamamoto DS, Genshi K, Otsuka H, One Y, Yusuf Y, Islam A, Yoshida S.
    • Journal Title

      Parasite Immunology

      Volume: 1 Issue: 5 Pages: 1-1

    • DOI

      10.1111/pim.12624

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 双翅目昆虫の多様な性決定様式2019

    • Author(s)
      山本大介
    • Journal Title

      昆虫と自然

      Volume: 印刷中

    • Related Report
      2018 Research-status Report
  • [Journal Article] Baculovirus-Induced Fast-Acting Innate Immunity Kills Liver-Stage Plasmodium.2018

    • Author(s)
      Emran TB, Iyori M, Ono Y, Amelia F, Yusuf Y, Islam A, Alam A, Tamura M, Ogawa R, Matsuoka H, Yamaoto DS, Yoshida S.
    • Journal Title

      The Journal of Immunology

      Volume: 201 Issue: 8 Pages: 2441-2441

    • DOI

      10.4049/jimmunol.1800908

    • Related Report
      2018 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Malaria infectivity of xanthurenic acid-deficient anopheline mosquitoes produced by TALEN-mediated targeted mutagenesis.2018

    • Author(s)
      Yamamoto DS, Sumitani M, Hatakeyama M, Matsuoka H
    • Journal Title

      Transgenic Research

      Volume: 27 Issue: 1 Pages: 51-60

    • DOI

      10.1007/s11248-018-0057-2

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Adenovirus-prime and baculovirus-boost heterologous immunization achieves sterile protection against malaria sporozoite challenge in a murine model.2018

    • Author(s)
      Yoshida K, Iyori M, Blagborough AM, Salman AM, Dulal P, Sala KA, Yamamoto DS, Khan SM, Janse CJ, Biswas S, Yoshii T, Yusuf Y, Tokoro M, Hill AVS, Yoshida S
    • Journal Title

      Scientific Reports

      Volume: 8 Issue: 1 Pages: 3896-3896

    • DOI

      10.1038/s41598-018-21369-y

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] DAF-shielded baculovirus-vectored vaccine enhances protection against malaria sporozoite challenge in mice.2017

    • Author(s)
      Iyori M, Yamamoto DS, Sakaguchi M, Mizutani M, Ogata S, Nishiura H, Tamura T, Matsuoka H, Yoshida S
    • Journal Title

      Malaria Journal

      Volume: 16 Issue: 1 Pages: 390-390

    • DOI

      10.1186/s12936-017-2039-x

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] ハエ目(双翅目)におけるゲノム編集研究2017

    • Author(s)
      山本大介
    • Journal Title

      Sanshi-Konchu Biotec

      Volume: 86 Issue: 2 Pages: 2_105-2_114

    • DOI

      10.11416/konchubiotec.86.2_105

    • ISSN
      1881-0551, 1884-7943
    • Related Report
      2017 Research-status Report
  • [Journal Article] Inhibition of Malaria Infection in Transgenic Anopheline Mosquitoes Lacking Salivary Gland Cells.2016

    • Author(s)
      Yamamoto DS, Sumitani M, Kasashima K, Sezutsu H, Matsuoka H.
    • Journal Title

      PLoS Pathog.

      Volume: 12 Issue: 9 Pages: e1005872-e1005872

    • DOI

      10.1371/journal.ppat.1005872

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] 遺伝子改変技術を利用したハマダラカ唾液腺の研究2016

    • Author(s)
      山本大介
    • Journal Title

      医学のあゆみ

      Volume: 259

    • Related Report
      2016 Research-status Report
  • [Presentation] ハマダラカの吸血・病原体媒介機構の研究2019

    • Author(s)
      山本大介
    • Organizer
      第71回日本衛生動物学会大会(招待講演)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] 性分化遺伝子の制御を受けるハマダラカ血液消化関連遺伝子の探索2019

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第55回日本節足動物発生学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ハマダラカ雌におけるdoublesex遺伝子欠損の吸血・血液消化への影響2019

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第90回日本動物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] マウスBax遺伝子を利用した新しい細胞死誘導システム2019

    • Author(s)
      炭谷めぐみ・山本大介・笠嶋克巳・山田信人・坪田拓也・飯塚哲也・瀬筒秀樹
    • Organizer
      第90回日本動物学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ハマダラカにおける性分化制御の研究2019

    • Author(s)
      山本大介
    • Organizer
      第75回日本寄生虫学会西日本支部大会(招待講演)
    • Related Report
      2019 Annual Research Report
    • Invited
  • [Presentation] PCR-RFLP法を用いたエクアドルに分布するリーシュマニア原虫種の同定2019

    • Author(s)
      加藤大智・Eduardo A. Gomez・関千里・Ahmed Tabbabi・山本大介・橋口義久
    • Organizer
      第79回日本寄生虫学会東日本支部大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] ペルー共和国におけるリーシュマニア原虫の地理的分布2019

    • Author(s)
      加藤大智・Abraham G. Caceres・関千里・久保誠・Ahmed Tabbabi・山本大介・橋口義久
    • Organizer
      第60回日本熱帯医学会大会
    • Related Report
      2019 Annual Research Report
  • [Presentation] doublesexノックアウトハマダラカの中腸における遺伝子転写産物の解析2019

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第63回日本応用動物昆虫学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ハマダラカにおける性分化遺伝子をノックアウトした系統の解析2018

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第70回日本衛生動物学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] 唾液タンパクAAPPを欠損したトランスジェニックハマダラカの吸血行動の著しい制約2018

    • Author(s)
      Islam Ashekul, 伊従 光洋, 山本 大介, Talha Bin Emran, 都野 展子, 山口 莉理夏, Fitri Amelia, Yenni Yusuf, Henrique Silveira, 吉田 栄人
    • Organizer
      第70回日本衛生動物学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ステフェンスハマダラカ doublesex ホモログの雌特異的アイソフォーム欠損系統の解析2018

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第54回日本節足動物発生学会大会
    • Related Report
      2018 Research-status Report
  • [Presentation] ハマダラカにおける性分化制御による吸血・血液消化に関わる遺伝子の探索2018

    • Author(s)
      山本大介
    • Organizer
      ROIS-DS-JOINT共同研究集会「昆虫のゲノムデータベースとそれを活用したデータ解析II」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] 感染症媒介蚊におけるゲノム編集研究の現状2018

    • Author(s)
      山本大介
    • Organizer
      農研機構シンポジウム「機能改変昆虫の産業利用の可能性」
    • Related Report
      2018 Research-status Report
    • Invited
  • [Presentation] Efforts for safe use of gene drive technology in Japan2018

    • Author(s)
      Tanaka T, Tanaka N, Nagano Y, Kanuka H, Yamamoto DS, Yamamoto N, Nanba E, Nishiuchi T
    • Organizer
      The 5th Asian Conference on Safety and Education in Laboratory
    • Related Report
      2018 Research-status Report
    • Int'l Joint Research
  • [Presentation] ハマダラカにおけるdoublesex遺伝子ノックアウト2018

    • Author(s)
      山本大介・炭谷めぐみ・加藤大智
    • Organizer
      第62回日本応用動物昆虫学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ハエ目昆虫ハマダラカにおけるゲノム編集の利用2018

    • Author(s)
      山本大介
    • Organizer
      平成30年度蚕糸・昆虫機能利用学術講演会(第88回日本蚕糸学会大会)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] ハマダラカにおけるマウス Bax 遺伝子を用いた細胞死誘導系:不妊化技術への応用例2017

    • Author(s)
      山本大介・炭谷めぐみ・笠嶋克巳・瀬筒秀樹・松岡裕之・加藤大智
    • Organizer
      第61回日本応用動物昆虫学会大会
    • Place of Presentation
      小金井宮地楽器ホール・小金井
    • Year and Date
      2017-03-27
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞死誘導システムを利用したマラリア媒介蚊の解析とベクターコントロールへの展開2017

    • Author(s)
      山本大介・炭谷めぐみ
    • Organizer
      熱帯医学会・渡航医学会・国際保健医療学会合同大会(グローバルヘルス合同大会2017)
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] 病原体媒介蚊におけるゲノム編集研究の状況 ー遺伝子解析からGene Drive技術までー2017

    • Author(s)
      山本大介
    • Organizer
      第9回 遺伝子組換え実験安全研修会
    • Related Report
      2017 Research-status Report
    • Invited
  • [Presentation] Transgene mediated inhibition of collagen-induced platelet aggregation by Anopheline Anti-Platelet Protein, regulates probing time and blood feeding success in malaria vector mosquito Anopheles stephensi2017

    • Author(s)
      Islam Ashekul, Talha Bin Emran, Daisuke Yamamoto, Mitsuhiro Iyori, Fitri Amelia, Yenni Yusuf, Nobuko Tuno, Shigeto Yoshida
    • Organizer
      第35回北陸病害動物研究会
    • Related Report
      2017 Research-status Report
  • [Presentation] 細胞死誘導法によるハマダラカの雄不妊化の効果2017

    • Author(s)
      山本大介・炭谷めぐみ・笠嶋克巳・瀬筒秀樹・松岡裕之・加藤大智
    • Organizer
      第53回日本節足動物発生学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 精巣における細胞死誘導系を導入したハマダラカの解析2017

    • Author(s)
      山本大介・炭谷めぐみ・笠嶋克巳・瀬筒秀樹・松岡裕之・加藤大智
    • Organizer
      第69回日本衛生動物学会大会
    • Related Report
      2017 Research-status Report
  • [Presentation] ゲノム編集技術を利用したハマダラカにおけるマラリア寄生関連遺伝子の解析2016

    • Author(s)
      山本大介
    • Organizer
      第87回日本動物学会大会シンポジウム
    • Place of Presentation
      沖縄コンベンションセンター・宜野湾
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
    • Invited
  • [Presentation] Adaptation to an inducible tissue-specific cell death system in the malaria vector, Anopheles stephensi: a case of the salivary gland2016

    • Author(s)
      Yamamoto DS, Sumitani M, Kasashima K, Sezutsu H, Matsuoka H
    • Organizer
      XXV International Congress of Entomology (ICE)
    • Place of Presentation
      Orange County Convention Center, Florida, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Conservation of Mos/MAPK signaling pathway during insect oocyte maturation: a case study in the silkworm, Bombyx mori2016

    • Author(s)
      Megumi Sumitani, Daisuke S. Yamamoto, Hideki Sezutsu, Masatsugu Hatakeyama
    • Organizer
      XXV International Congress of Entomology (ICE)
    • Place of Presentation
      Orange County Convention Center, Florida, USA
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] マウス細胞死誘導因子を利用したハマダラカの組織機能解析2016

    • Author(s)
      山本大介
    • Organizer
      昆虫デザイン研究会
    • Place of Presentation
      自然科学研究機構基礎生物学研究所・岡崎
    • Year and Date
      2016-07-07
    • Related Report
      2016 Research-status Report
  • [Presentation] 細胞死誘導系を利用したハマダラカにおける唾液腺機能解析2016

    • Author(s)
      山本大介・炭谷めぐみ・笠嶋克巳・瀬筒秀樹・松岡裕之
    • Organizer
      第52回日本節足動物発生学会大会
    • Place of Presentation
      湘南国際村センター・神奈川県葉山
    • Year and Date
      2016-06-10
    • Related Report
      2016 Research-status Report
  • [Presentation] 唾液腺細胞で細胞死を誘導したハマダラカにおけるマラリア感染解析2016

    • Author(s)
      山本大介・炭谷めぐみ・笠嶋克巳・瀬筒秀樹・松岡裕之
    • Organizer
      第68回日本衛生動物学会大会
    • Place of Presentation
      栃木県総合文化センター・宇都宮
    • Year and Date
      2016-04-16
    • Related Report
      2016 Research-status Report
  • [Remarks] 唾液の無い蚊はマラリアにほとんど感染しない

    • URL

      http://www.jichi.ac.jp/news/research/2016/20160907.html

    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2021-02-19  

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