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Investigation of mechanism of anhydrobiosis by using culture cell of aleeping chironomid

Research Project

Project/Area Number 16K18827
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Insect science
Research InstitutionFukushima National College of Technology

Principal Investigator

Sogame Yoichiro  福島工業高等専門学校, 化学・バイオ工学科, 助教 (60769850)

Research Collaborator Kikawada Takahiro  
Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2018: ¥650,000 (Direct Cost: ¥500,000、Indirect Cost: ¥150,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Keywords無代謝休眠 / シスト / 環境ストレス / ストレス応答 / 昆虫細胞 / 遺伝子発現 / 遺伝子サイレンシング / 環境耐性 / 極限環境
Outline of Final Research Achievements

In this study, the molecular mechanisms for anhydrobiosis were researched by using culture cells of sleeping chironomid. First stage, experiment techniques were established in the cells. In second stage, promoter which function in the cells was identified. After the movement of institute, investigation of molechular mechanisms by using single cell animal was performed.

Academic Significance and Societal Importance of the Research Achievements

無代謝休眠は、極限環境などに生息する特殊な生物に見られる生命現象である。無代謝休眠の状態では、代謝は停止している一方、乾燥をはじめ様々な環境ストレスに対する耐性を有している。この現象の分子機構解明は、生命現象の仕組みの追求だけにとどまらず、生物や通常乾燥保存できないような物質の保存など様々な分野で応用が可能である。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (42 results)

All 2019 2018 2017 2016

All Journal Article (9 results) (of which Int'l Joint Research: 7 results,  Peer Reviewed: 8 results,  Open Access: 6 results,  Acknowledgement Compliant: 2 results) Presentation (33 results) (of which Int'l Joint Research: 14 results,  Invited: 2 results)

  • [Journal Article] Identification of a Novel Strong Promoter from the Anhydrobiotic Midge, Polypedilum vanderplanki, with Conserved Function in Various Insect Cell Lines2019

    • Author(s)
      Miyata Y.*, Tokumoto S.*, Sogame Y.*,(equal contribution) Deviatiiarov R., Okada J., Cornette R., Gusev O., Shagimardanova E , Sakurai M., Kikawada T
    • Journal Title

      Scientific reports

      Volume: 9 Pages: 7004-7004

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 眠る昆虫ネムリユスリカの乾燥耐性:アンヒドロビオシスのしくみ2019

    • Author(s)
      十亀 陽一郎・黄川田隆洋
    • Journal Title

      昆虫と自然

      Volume: 54 Pages: 8-11

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Co-option of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki2018

    • Author(s)
      Pavel V Mazin, Elena Shagimardanova, Olga Kozlova, Alexander Cherkasov, Roman Sutormin, Vita Stupnikov, Maria Logacheva, Aleksey Penin, Yoichiro Sogame, Richard Cornette, Shoko Tokumoto, Yugo Miyata, Takahiro Kikawada, Mikhail Gelfand, Oleg Gusev
    • Journal Title

      PNAS

      Volume: 115 Pages: 2477-2486

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Co-option of heat shock regulatory system for anhydrobiosis in the sleeping chironomid Polypedilum vanderplanki2018

    • Author(s)
      Pavel V Mazin, Elena Shagimardanova, Olga Kozlova, Alexander Cherkasov, Roman Sutormin, Vita Stupnikov, Maria Logacheva, Aleksey Penin, Yoichiro Sogame, Richard Cornette, Shoko Tokumoto, Yugo Miyata, Takahiro Kikawada, Mikhail Gelfand, Oleg Gusev
    • Journal Title

      Proceedings of the National Academy of Sciences of the United States of America

      Volume: 115 Pages: 2477-2486

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] 真核単細胞生物コルポーダの休眠機構解明に向けた生理・生化学的アップローチ2018

    • Author(s)
      十亀陽一郎・松岡達臣
    • Journal Title

      比較生理生化学

      Volume: 34 Pages: 116-122

    • Related Report
      2017 Research-status Report
    • Peer Reviewed
  • [Journal Article] Towards water-free biobanks: long-term dry-preservation at room temperature of desiccation-sensitive enzyme luciferase in air-dried insect cells2018

    • Author(s)
      Kikuta S, Watanabe J S, Sato R, Gusev O, Nesmelov A, Sogame Y, Cornette R, Kikawada T
    • Journal Title

      Scientific Reports

      Volume: 7 Pages: 6540-6540

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Current findings on the molecular mechanisms underlying anhydrobiosis in Polyprdilum vanderplanki2017

    • Author(s)
      Sogame Y, Kikawada T
    • Journal Title

      Current Opinion in Insect Science

      Volume: 19 Pages: 16-21

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Establishment of gene transfer and gene silencing methods in a desiccation‐tolerant cell line, Pv112017

    • Author(s)
      Yoichiro Sogame, Jun Okada, Shingo Kikuta, Yugo Miyata, Richard Cornette, Oleg Gusev, Takahiro Kikawada
    • Journal Title

      Extremophiles

      Volume: 21 Issue: 1 Pages: 65-72

    • DOI

      10.1007/s00792-016-0880-4

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Morphogenetic and molecular analyses of cyst wall components in the ciliated protozoan Colpoda cucullus Nag-1.2017

    • Author(s)
      Funadani R*, Sogame Y*,(*equal contributor) Kojima K, Takeshita T, Yamamoto K, Tsujizono T, Suizu F, Miyata S, Yagyu KI, Suzuki T, Arikawa M, Matsuoka T
    • Journal Title

      FEMS microbiology letters

      Volume: 363 Issue: 18 Pages: fnw203-fnw203

    • DOI

      10.1093/femsle/fnw203

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] A New Insight into Cryptobiosis- Cell Repair in Ametabolism2019

    • Author(s)
      Yoichiroo Sogame, Ryota Saito, Ryota Koizumi
    • Organizer
      ICAP2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Tolerance to Ionizing radiation and Irradiation Damage Repair in the Unicellular Eukaryote Colpoda cucullus2019

    • Author(s)
      Yoichiro SOGAME, Ryota SAITO, Ryota KOIZUMI, Taiga SHIMIZU, Tatsuya SAKAI, Taiki ONO, Toshio TAKYU
    • Organizer
      wet2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Extreme Gamma Radiation Tolerance of Colpoda cucullus: Physiological and Morphological Analysis of Irradiated Cysts2019

    • Author(s)
      Ryota SAITO, Ryota KOIZUMI, Taiga SHIMIZU, Taiki ONO, Tatsuya SAKAI, Toshio TAKYU, Yoichiro SOGAME
    • Organizer
      wet2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effects of Cell Repair on the Excystment of Irradiated Cysts and Cell Growth of Vegetative Cells Excysted from Irradiated Cysts in Colpoda cucullus2019

    • Author(s)
      Ryota Koizumi, Ryota Saito, Taiga Shimizu, Toshio Takyu, Yoichiro Sogame
    • Organizer
      wet2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Morphological Events in the Formation of Cyst-specific Cell Structures and Acquisition of Autofluorescence during the Encystment Process in Colpoda cucullus2019

    • Author(s)
      Tatsuya SAKAI, Taiga SHIMIZU, Ryota KOIZUMI, Ryota SAITO, Taiki ONO, Yoichiro SOGAME
    • Organizer
      wet2019
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 水性単細胞生物Colpodaの放射線耐性:ガンマ線照射の脱シストと細胞増殖に与える影響2019

    • Author(s)
      十亀陽一郎・齊藤瞭汰・小泉亮太・清水大雅・酒井達弥
    • Organizer
      第53回日本水環境学会年会
    • Related Report
      2018 Annual Research Report
  • [Presentation] Gamma Radiation Tolerance and Influence on Cell Growth in Unicellular Colpoda sp2018

    • Author(s)
      Ryota KOIZUMI, Taiki ONO, Ryota SAITO, Taiga SHIMIZU, Tatsuya SAKAI, Yoichiro SOGAME
    • Organizer
      wet2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Comparative Analysis: Tolerance for Varying Amount of Gamma Radiation in Eukaryote Colpoda sp. Vegetative Cells vs. Resting Cysts2018

    • Author(s)
      Ryota SAITO, Ryota KOIZUMI, Yoichiro SOGAME
    • Organizer
      wet2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of 16S rRNA Genes of Symbiotic Luminous Bacteria Coexisting with Deep Sea Fish Chlorophthalmus2018

    • Author(s)
      Taiga SHIMIZU, Ryota SAITO, Ryota KOIZUMI, Masahide HAGIRI, Kaito MIZUMACH, Yoichiro SOGAME
    • Organizer
      wet2018
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 単細胞生物コルポーダの放射線耐性に関する研究2018

    • Author(s)
      十亀陽一郎 小泉亮太 小野泰輝 武弓利雄
    • Organizer
      第52回日本水環境学会年会
    • Related Report
      2017 Research-status Report
  • [Presentation] 農地環境に適応した単細胞生物の放射線耐性に関する研究2018

    • Author(s)
      小泉亮太 小野泰輝 齊藤瞭汰 十亀陽一郎
    • Organizer
      土木学会東北支部技術研究発表会
    • Related Report
      2017 Research-status Report
  • [Presentation] いわき市シティーフィッシュ“メヒカリ”に共生する発光バクテリアの採集と同定2018

    • Author(s)
      清水大雅 水町海斗 十亀 陽一郎
    • Organizer
      土木学会東北支部技術研究発表会
    • Related Report
      2017 Research-status Report
  • [Presentation] 発光バクテリアを用いた下水水質評価と下水管きょ劣化評価へのアプローチ2018

    • Author(s)
      十亀 陽一郎・江本久雄
    • Organizer
      土木学会東北支部技術研究発表会
    • Related Report
      2017 Research-status Report
  • [Presentation] 身近に生息する生物の放射線吸収量の定量解析と放射線耐性について2018

    • Author(s)
      鈴木喬也・武弓利夫・十亀 陽一郎
    • Organizer
      原子力規制人材育成事業・教育研究フォーラム 地域の環境回復と環境安全に貢献できる原子力規制人材の育成
    • Related Report
      2017 Research-status Report
  • [Presentation] 身近な水環境に生息する単細胞生物の放射線耐性に関する研究2017

    • Author(s)
      十亀 陽一郎
    • Organizer
      平成29年度第2ブロック研究情報交換会
    • Related Report
      2017 Research-status Report
  • [Presentation] 身近に生息する微生物の環境ストレス耐性2017

    • Author(s)
      鈴木喬也・武弓利雄・十亀陽一郎
    • Organizer
      第3回北関東磐越地区化学技術フォーラム
    • Related Report
      2017 Research-status Report
  • [Presentation] 発光バクテリアを用いた環境評価システムの構築2017

    • Author(s)
      佐藤暁 江本久雄 十亀陽一郎
    • Organizer
      第3回北関東磐越地区化学技術フォーラム
    • Related Report
      2017 Research-status Report
  • [Presentation] 佐藤暁 江本久雄 十亀陽一郎2017

    • Author(s)
      発光バクテリアの発光強度による下水水質評価方法の検討
    • Organizer
      第8回福島地区CEセミナー
    • Related Report
      2017 Research-status Report
  • [Presentation] 発光バクテリアの発光強度による下水管評価及びそのデータベース構築2017

    • Author(s)
      江本久雄, 十亀 陽一郎
    • Organizer
      第42回土木情報学シンポジウム 2017年9月
    • Related Report
      2017 Research-status Report
  • [Presentation] アミガサタケ属(Morchella)のキノコを用いた環境放射能の 定量解析2017

    • Author(s)
      鈴木喬也, 佐藤佳子, 江本久雄, 北村早苗, 櫛田拳, 齋藤詩乃, 佐藤暁, 冨川康之, 十亀陽一郎
    • Organizer
      日本きのこ学会第21回大会
    • Related Report
      2017 Research-status Report
  • [Presentation] 福島県に自生するキノコの含有放射性物質濃度の解析2017

    • Author(s)
      鈴木喬也 ,佐藤佳子,江本久雄 ,北村早苗 ,櫛田拳 , 齋藤詩乃, 佐藤暁 ,十亀陽一郎
    • Organizer
      日本地球化学会第64回年会
    • Related Report
      2017 Research-status Report
  • [Presentation] Pv11 cells as an effective tool to investigate molecular mechanisms underlying the extreme desiccation tolerance, anhydrobiosis2017

    • Author(s)
      Sogame Y., Miyata Y., Kikuta S., Cornette R., Oleg Gusev O., Kikawada T.
    • Organizer
      ICAP2017
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research / Invited
  • [Presentation] Analysis of a powerful constitutive Promoter in Pv11 cells: Establishment of a strong constitutive gene expression vector for insect cells2017

    • Author(s)
      Sogame Y., Miyata Y., Deviatiiarov R., Kikuta, S., Richard, C., Gusev, O., Kikawada
    • Organizer
      ICAP2017
    • Related Report
      2017 Research-status Report
  • [Presentation] ネムリユスリカPolypedilum vanderplankiのゲノムアセンブリ2017

    • Author(s)
      尾納 隆大, 山口 勝司, 十亀 陽一郎, 黄川田 隆洋, 重信 秀治
    • Organizer
      NGS field meeting
    • Related Report
      2017 Research-status Report
  • [Presentation] Genome Editing in a Cell Line of the Sleeping Chironomid by Using the CRISPR/Cas9 System2016

    • Author(s)
      Miyata Y, Sogame Y, Tokumoto S, Sakurai M, Kikawada T
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      神奈川
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] The sleeping chironomid as a model organism to investigate molecular mechanisms underlying anhydrobiosis2016

    • Author(s)
      Kikawada T, Miyata Y, Sogame Y, Cornrtte R, Gusev O
    • Organizer
      第39回日本分子生物学会
    • Place of Presentation
      神奈川
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 繊毛虫コルポーダ Colpoda cucullus における休眠シスト壁形成に関する研究2016

    • Author(s)
      舟谷 亮二, 十亀 陽一郎, 小嶋 克彦, 竹下 敏一, 山本 圭祐, 辻囿 智哉, 水津 太, 宮田 清司, 矢生 健一, 鈴木 知彦, 有川 幹彦, 松岡 達臣
    • Organizer
      第49回 日本原生生物学会
    • Place of Presentation
      岡山
    • Year and Date
      2016-10-08
    • Related Report
      2016 Research-status Report
  • [Presentation] Identification of a strong promoter in cultured cells of Polypedilum vanderplanki as a tool to reveal molecular background underlying anhydrobiosis2016

    • Author(s)
      Sogame Y, Okada J, Deviatiiarov R, Kikuta S,Cornette R, Gusev O, Kikawada T
    • Organizer
      25th International Congress of Entomology
    • Place of Presentation
      アメリカ
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] The first edition of mutagenesis by CRISPR/Cas in the extreme desiccation tolerant cultured cell.2016

    • Author(s)
      Kikawada T, Miyata Y, Sogame Y, Furusawa T, Kikuta S, Cornette R, Gusev O
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analyses on the mechanism of induction of anhydrobiosis in the midge Polypedilum vanderplanki2016

    • Author(s)
      Cornette R, Iwata K-I, Kikuta S, Sogame Y, Okuda T, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Analysis of a powerful constitutive promoter in culture cells of Polypedilum vanderplanki.2016

    • Author(s)
      Sogame Y, Miyata Y, Deviatiiarov R, Kikuta S, Cornette R, Gusev O, Furusawa T, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Anhydro-preservation of exogenously-expressed desiccation-sensitive enzyme Luciferase using insect cells2016

    • Author(s)
      Kikuta S, Watanabe S, Gusev O, Sogame Y, Cornett R, Kikawada T
    • Organizer
      10th International Conference On Bioinformatics of Genome Regulation and Structure Systems Biology
    • Place of Presentation
      ロシア
    • Year and Date
      2016-08-29
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] 乾燥状態のネムリユスリカ由来培養細胞は外来性酵素を保護する2016

    • Author(s)
      渡辺俊介, 菊田 真吾, Oleg Gusev, 十亀陽一郎, 佐藤令一, Richard Cornette, 黄川田隆洋
    • Organizer
      第61回低温生物工学会大会
    • Place of Presentation
      埼玉
    • Year and Date
      2016-06-25
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2020-03-30  

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