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Analysis of physiological role of early endosome motility in the "Japanese national fungus" Aspergillus oryzae

Research Project

Project/Area Number 16K18837
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Applied molecular and cellular biology
Research InstitutionKyushu University

Principal Investigator

Higuchi Yujiro  九州大学, 農学研究院, 助教 (50732765)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
Keywords黄麹菌 / Aspergillus oryzae / 初期エンドソーム / 細胞内動態 / AoHok1 / αーアミラーゼ / タンパク質分泌 / 細胞分化 / α―アミラーゼ / 二次代謝産物 / コウジ酸 / 細胞内膜輸送 / 国菌
Outline of Final Research Achievements

Recently, it has revealed that the motility of an endocytic organelle early endosome (EE) has a variety of physiological roles. To further examine the role of the EE motility in the industrially important fungus Aspergillus oryzae, AoHok1, a putative linker protein between an EE and a motor protein was identified. The Aohok1 disruptant showed retarded mycelial growth and no EE motility, in addition to an apical accumulation of EEs and peroxisomes. Analyses on the protein secretory pathway in ΔAohok1 cells showed that, although distribution of the endoplasmic reticulum and Golgi was not affected, formation of the apical secretory vesicle cluster was impaired, resulting in the reduction of the major secretory protein α-amylase. Moreover, the transcript level of α-amylase-encoding gene amyB was significantly reduced in the Aohok1 disruptant. Furthermore, perturbed conidial and sclerotial formations were observed, indicating a defect in cell differentiation, in the Aohok1 disruptant.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016 Other

All Journal Article (7 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 7 results,  Open Access: 3 results,  Acknowledgement Compliant: 1 results) Presentation (9 results) Remarks (2 results)

  • [Journal Article] Early endosome motility mediates α-amylase production and cell differentiation in Aspergillus oryzae2017

    • Author(s)
      Togo Yusuke、Higuchi Yujiro、Katakura Yoshinori、Takegawa Kaoru
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 15757-15757

    • DOI

      10.1038/s41598-017-16163-1

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Analysis of an acyl-CoA binding protein in Aspergillus oryzae that undergoes unconventional secretion2017

    • Author(s)
      Kwon Hee Su、Kawaguchi Kouhei、Kikuma Takashi、Takegawa Kaoru、Kitamoto Katsuhiko、Higuchi Yujiro
    • Journal Title

      Biochem Biophys Res Commun.

      Volume: 493 Issue: 1 Pages: 481-486

    • DOI

      10.1016/j.bbrc.2017.08.166

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline2017

    • Author(s)
      Yujiro Higuchi, Yasunari Eshima, Yibo Huang, Takashi Kinoshita, Wataru Sumiyoshi, Shin-ichi Nakakita, Kaoru Takegawa
    • Journal Title

      Biotechnology Letters

      Volume: 39 Issue: 1 Pages: 157-162

    • DOI

      10.1007/s10529-016-2230-0

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Highly efficient transglycosylation of sialo-complex-type oligosaccharide using Coprinopsis cinerea endoglycosidase and sugar oxazoline.2017

    • Author(s)
      Higuchi Y, Eshima Y, Huang Y, Kinoshita T, Sumiyoshi W, Nakakita S, Takegawa K
    • Journal Title

      Biotechnology Letters

      Volume: 39 Issue: 1 Pages: 8-12

    • DOI

      10.1016/j.bbrc.2016.10.018

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Co-delivery of cell-wall-forming enzymes in the same vesicle for coordinated fungal cell wall formation2016

    • Author(s)
      Martin Schuster, Magdalena Martin-Urdiroz, Yujiro Higuchi, Christian Hacker, Sreedhar Kilaru, Sarah Gurr, Gero Steinberg
    • Journal Title

      Nature Microbiology

      Volume: 1 Issue: 11 Pages: 16149-16149

    • DOI

      10.1038/nmicrobiol.2016.149

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] A rationally engineered yeast pyruvyltransferase Pvg1p introduces sialylation-like properties in neo-human-type complex oligosaccharide.2016

    • Author(s)
      Higuchi Y, Yoshinaga S, Yoritsune K, Tateno H, Hirabayashi J, Nakakita S, Kanekiyo M, Kakuta Y, Takegawa K.
    • Journal Title

      Sci Rep

      Volume: 6 Issue: 1 Pages: 26349-26349

    • DOI

      10.1038/srep26349

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access
  • [Journal Article] Draft genome sequence of Bacillus clausii AKU0647, a strain that produces Endo-β-N-Acetylglucosaminidase A2016

    • Author(s)
      Yujiro Higuchi, Kazuki Mori, Akiko Suyama, Yibo Huang, Kosuke Tashiro, Satoru Kuhara, Kaoru Takegawa
    • Journal Title

      Genome Announcements

      Volume: 4 Issue: 3

    • DOI

      10.1128/genomea.00310-16

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Presentation] 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態のコウジ酸分泌への関与2018

    • Author(s)
      都甲 祐介, 竹川 薫, 樋口 裕次郎
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 糸状菌における細胞内膜輸送及び動態の生理学的意義に関する研究2017

    • Author(s)
      樋口 裕次郎
    • Organizer
      日本農芸化学会西日本支部例会
    • Place of Presentation
      九州大学
    • Year and Date
      2017-01-21
    • Related Report
      2016 Research-status Report
  • [Presentation] 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態の有用物質生産への関与2017

    • Author(s)
      都甲 祐介, 竹川 薫, 樋口 裕次郎
    • Organizer
      第17回糸状菌分子生物学コンファレンス
    • Related Report
      2017 Annual Research Report
  • [Presentation] 黄麹菌Aspergillus oryzaeの固体培養における初期エンドソーム動態に関する解析2017

    • Author(s)
      都甲 祐介, 竹川 薫, 樋口 裕次郎
    • Organizer
      日本農芸化学会 関西・中四国・西日本支部 2017年度合同大阪大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 黄麹菌Aspergillus oryzaeにおける分泌糖タンパク質に関する解析2017

    • Author(s)
      李 秋実, 竹川 薫, 樋口 裕次郎
    • Organizer
      第69回 日本生物工学会大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 黄麹菌Aspergillus oryzaeにおいて初期エンドソーム動態は細胞の分化制御およびタンパク質分泌に関与する2017

    • Author(s)
      樋口 裕次郎, 都甲 祐介, 竹川 薫
    • Organizer
      日本農芸化学会
    • Related Report
      2017 Annual Research Report 2016 Research-status Report
  • [Presentation] 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態に関する解析2016

    • Author(s)
      都甲 祐介, 竹川 薫, 樋口 裕次郎
    • Organizer
      第16回糸状菌分子生物学コンファレンス
    • Place of Presentation
      京都大学
    • Year and Date
      2016-11-17
    • Related Report
      2016 Research-status Report
  • [Presentation] 黄麹菌Aspergillus oryzaeにおける初期エンドソーム動態関連因子の解析2016

    • Author(s)
      都甲 祐介, 竹川 薫, 樋口 裕次郎
    • Organizer
      日本農芸化学会 西日本支部大会
    • Place of Presentation
      長崎大学
    • Year and Date
      2016-09-16
    • Related Report
      2016 Research-status Report
  • [Presentation] 糸状菌の細胞内膜輸送に関する研究2016

    • Author(s)
      樋口 裕次郎
    • Organizer
      日本農芸化学会 西日本支部創立80周年記念シンポジウム
    • Place of Presentation
      長崎大学
    • Year and Date
      2016-09-15
    • Related Report
      2016 Research-status Report
  • [Remarks] 九州大学発酵化学研究室

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/hakko/

    • Related Report
      2017 Annual Research Report
  • [Remarks] 九州大学発酵化学研究室HP

    • URL

      http://www.agr.kyushu-u.ac.jp/lab/hakko/

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

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