Analysis of physiological role of early endosome motility in the "Japanese national fungus" Aspergillus oryzae
| Project/Area Number |
16K18837
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000)
Fiscal Year 2016: ¥3,380,000 (Direct Cost: ¥2,600,000、Indirect Cost: ¥780,000)
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| Keywords | 黄麹菌 / Aspergillus oryzae / 初期エンドソーム / 細胞内動態 / AoHok1 / αーアミラーゼ / タンパク質分泌 / 細胞分化 / α―アミラーゼ / 二次代謝産物 / コウジ酸 / 細胞内膜輸送 / 国菌 |
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| Outline of Final Research Achievements |
Recently, it has revealed that the motility of an endocytic organelle early endosome (EE) has a variety of physiological roles. To further examine the role of the EE motility in the industrially important fungus Aspergillus oryzae, AoHok1, a putative linker protein between an EE and a motor protein was identified. The Aohok1 disruptant showed retarded mycelial growth and no EE motility, in addition to an apical accumulation of EEs and peroxisomes. Analyses on the protein secretory pathway in ΔAohok1 cells showed that, although distribution of the endoplasmic reticulum and Golgi was not affected, formation of the apical secretory vesicle cluster was impaired, resulting in the reduction of the major secretory protein α-amylase. Moreover, the transcript level of α-amylase-encoding gene amyB was significantly reduced in the Aohok1 disruptant. Furthermore, perturbed conidial and sclerotial formations were observed, indicating a defect in cell differentiation, in the Aohok1 disruptant.
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Report
(3 results)
Research Products
(18 results)
