DNA methylation regulated by plant proteasome

• Project/Area Number: 16K18838
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied molecular and cellular biology
• Research Institution: Institute of Physical and Chemical Research
• Principal Investigator: Sako Kaori 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (60722395)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
• Keywords: プロテアソーム / DNAメチル化 / 植物

Outline of Final Research Achievements

DNA methylation plays an important role in gene regulation. On the other hand, hyper methylation gives an adverse effect in plants. We showed that a DNA methylation related protein was degraded by ubiquitin/proteasome system. And this protein negatively regulated gene expression in callus regeneration. Thus, proteasome degrades this protein for proper gene expression.

Academic Significance and Societal Importance of the Research Achievements

組換え作物に導入した有用遺伝子がDNAメチル化の過剰促進によってサイレンシングされる現象が問題となっている。本研究ではプロテアソームによるタンパク質分解が適切な遺伝子発現制御に寄与することを明らかにした。本研究を発展させることによって過剰なメチル化を抑制し，食糧生産性増進など，応用面への貢献が期待できる。

Research Products

• [Journal Article] The duration of ethanol-induced high-salinity stress tolerance in Arabidopsis thaliana (2018)
  • Author(s): Sako Kaori、Sunaoshi Yuji、Tanaka Maho、Matsui Akihiro、Seki Motoaki
  • Journal Title: Plant Signaling & Behavior Volume: 13 Pages: 1-3
  • DOI: 10.1080/15592324.2018.1500065
  • Peer Reviewed
• [Journal Article] ヒストン脱アセチル化酵素が担う植物の環境ストレス応答制御 植物をストレスに強くする標的タンパク質の発見 (2018)
  • Author(s): 上田実，佐古香織，伊藤昭博, 吉田稔，関原明
  • Journal Title: 化学と生物 Volume: 56 Pages: 797-803
  • NAID: 130007748619
  • Peer Reviewed
• [Journal Article] Transcriptomic analysis of Arabidopsis thaliana plants treated with the Ky-9 and Ky-72 histone deacetylase inhibitors (2018)
  • Author(s): Nguyen HM, Sako K, Matsui A, Ueda M, Tanaka M, Ito A, Nishino N, Yoshida M, Seki M.
  • Journal Title: Plant Signaling and Behavior Volume: 13 Issue: 3 Pages: e1448333-e1448333
  • DOI: 10.1080/15592324.2018.1448333
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Live imaging of H3K9 acetylation in plant cells. (2017)
  • Author(s): Kurita, K., Sakamoto,T., Yagi, N., Sakamoto, Y., Ito, A., Nishino, N., Sako,K., Yoshida, M., Kimura, H., Seki, M. and Matsunaga, S.
  • Journal Title: Scientific Reports Volume: 7 Issue: 1 Pages: 45894-45894
  • DOI: 10.1038/srep45894
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Ethanol Enhances High-Salinity Stress Tolerance by Detoxifying Reactive Oxygen Species in Arabidopsis thaliana and Rice (2017)
  • Author(s): Nguyen HM, Sako K, Matsui A, Suzuki Y, Mostofa MG, Ha CV, Tanaka M, Tran LP, Habu Y, Seki M
  • Journal Title: Frontiers in Plant Science Volume: 8 Pages: 1001-1001
  • DOI: 10.3389/fpls.2017.01001
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] The Distinct Roles of Class I and II RPD3-Like Histone Deacetylases in Salinity Stress Response (2017)
  • Author(s): Ueda M, Matsui A, Tanaka M, Nakamura T, Abe T, Sako K, Sasaki T, Kim JM, Ito A, Nishino N, Shimada H, Yoshida M, Seki M
  • Journal Title: Plant Physiology Volume: 175 Issue: 4 Pages: 1760-1773
  • DOI: 10.1104/pp.17.01332
  • Peer Reviewed / Open Access
• [Journal Article] Ky-2, a Histone Deacetylase Inhibitor, Enhances High-Salinity Stress Tolerance in Arabidopsis thaliana (2016)
  • Author(s): Kaori Sako, Jong-Myong Kim, Akihiro Matsui, Kotaro Nakamura, Maho Tanaka, Makoto Kobayashi, Kazuki Saito, Norikazu Nishino, Miyako Kusano, Teruaki Taji, Minoru Yoshida, Motoaki Seki
  • Journal Title: Plant and Cell Physiology Volume: 57 Issue: 4 Pages: 776-783
  • DOI: 10.2460/ajvr.77.4.346
  • Peer Reviewed
• [Journal Article] エピジェネティック制御によって植物の耐塩性を高める (2016)
  • Author(s): 佐古香織、関 原明
  • Journal Title: バイオサイエンスとインダストリー Volume: 74 Pages: 312-313
• [Presentation] Chemical screening of compounds enhancing salinity stress tolerance (2018)
  • Author(s): 佐古香織，チエン・バン・ハ，松井章浩，田中真帆，佐藤綾人，関原明
  • Organizer: 第60回日本植物生理学会
• [Presentation] Identification of a novel compound enhancing salinity stress tolerance (2017)
  • Author(s): Kaori Sako, Takeshi Shimizu, Kenshirou Shimizu, Hiroyuki Hirano, Akihiro Matsui, Minoru Ueda, Maho Tanaka, Hiroyuki Osada, Motoaki Seki
  • Organizer: 第58回日本植物生理学会
  • Place of Presentation: 鹿児島大学(鹿児島県鹿児島市)
  • Year and Date: 2017-03-17
• [Presentation] An HDAC inhibitor enhances salinity stress tolerance through promoted root barrier in Arabidopsis thaliana (2017)
  • Author(s): Kaori Sako, Huong Mai Nguyen, Minoru Ueda, Akihiro Matsui, Maho Tanaka, Norikazu Nishino, Minoru Yoshida, Motoaki Seki
  • Organizer: Environmental Stress Adaptation and Memory in Plants
  • Place of Presentation: 理化学研究所（神奈川県横浜市）
  • Year and Date: 2017-02-27
  • Int'l Joint Research / Invited
• [Presentation] 植物プロテアソームによる高塩ストレス応答機構の解析 (2017)
  • Author(s): 佐古香織
  • Organizer: ユビキチン研究会
• [Presentation] シロイヌナズナプロテアソームによるDNAメチル化制御機構の解析 (2016)
  • Author(s): 佐古 香織
  • Organizer: ユビキチンネオバイオロジー平成28年度班会議
  • Place of Presentation: シャトレーゼガトーキングダムサッポロ(北海道札幌市）
  • Year and Date: 2016-11-16
• [Book] Salinity Responses and Tolerance in Plants, Volume 2: Exploring RNAi, Genome Editing and Systems Biology (2018)
  • Author(s): Ueda M, Sako K, Seki M
  • Total Pages: 326
  • Publisher: Springer
  • ISBN: 9783319903170
• [Book] Exploring RNAi, Genome Editing and Systems Biology (2018)
  • Author(s): Ueda Minoru, Kaori Sako, Motoaki Seki
  • Total Pages: 500
  • Publisher: Springer
• [Remarks] 所属研究室のwebページ
  • URL: http://pgn.riken.jp/
• [Patent(Industrial Property Rights)] エタノールによる環境ストレス耐性付与 (2017)
  • Inventor(s): 関原明，佐古香織，松井章浩，Khurram Bashir他4名
  • Industrial Property Rights Holder: 関原明，佐古香織，松井章浩，Khurram Bashir他4名
  • Industrial Property Rights Type: 特許
  • Filing Date: 2017
  • Overseas
• [Patent(Industrial Property Rights)] 植物の環境ストレス耐性向上剤 (2016)
  • Inventor(s): 関原明、佐古香織、松井章浩、クーラムバシル他
  • Industrial Property Rights Holder: 関原明、佐古香織、松井章浩、クーラムバシル他
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-252242
  • Filing Date: 2016-12-27
• [Patent(Industrial Property Rights)] 植物の耐塩性向上剤 (2016)
  • Inventor(s): 関原明、佐古香織、長田裕之、清水猛、平野博之
  • Industrial Property Rights Holder: 関原明、佐古香織、長田裕之、清水猛、平野博之
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: 2016-193311
  • Filing Date: 2016-09-30