DNA methylation regulated by plant proteasome
| Project/Area Number |
16K18838
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied molecular and cellular biology
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| Research Institution | Institute of Physical and Chemical Research |
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Principal Investigator |
Sako Kaori 国立研究開発法人理化学研究所, 環境資源科学研究センター, 特別研究員 (60722395)
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| Project Period (FY) |
2016-04-01 – 2019-03-31
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| Project Status |
Completed (Fiscal Year 2018)
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| Budget Amount *help |
¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | プロテアソーム / DNAメチル化 / 植物 |
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| Outline of Final Research Achievements |
DNA methylation plays an important role in gene regulation. On the other hand, hyper methylation gives an adverse effect in plants. We showed that a DNA methylation related protein was degraded by ubiquitin/proteasome system. And this protein negatively regulated gene expression in callus regeneration. Thus, proteasome degrades this protein for proper gene expression.
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| Academic Significance and Societal Importance of the Research Achievements |
組換え作物に導入した有用遺伝子がDNAメチル化の過剰促進によってサイレンシングされる現象が問題となっている。本研究ではプロテアソームによるタンパク質分解が適切な遺伝子発現制御に寄与することを明らかにした。本研究を発展させることによって過剰なメチル化を抑制し，食糧生産性増進など，応用面への貢献が期待できる。
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Report
(4 results)
Research Products
(19 results)
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[Journal Article] Live imaging of H3K9 acetylation in plant cells.2017
Author(s)
Kurita, K., Sakamoto,T., Yagi, N., Sakamoto, Y., Ito, A., Nishino, N., Sako,K., Yoshida, M., Kimura, H., Seki, M. and Matsunaga, S.
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Journal Title
Scientific Reports
Volume: 7
Issue: 1
Pages: 45894-45894
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] The Distinct Roles of Class I and II RPD3-Like Histone Deacetylases in Salinity Stress Response2017
Author(s)
Ueda M, Matsui A, Tanaka M, Nakamura T, Abe T, Sako K, Sasaki T, Kim JM, Ito A, Nishino N, Shimada H, Yoshida M, Seki M
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Journal Title
Plant Physiology
Volume: 175
Issue: 4
Pages: 1760-1773
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ky-2, a Histone Deacetylase Inhibitor, Enhances High-Salinity Stress Tolerance in Arabidopsis thaliana2016
Author(s)
Kaori Sako, Jong-Myong Kim, Akihiro Matsui, Kotaro Nakamura, Maho Tanaka, Makoto Kobayashi, Kazuki Saito, Norikazu Nishino, Miyako Kusano, Teruaki Taji, Minoru Yoshida, Motoaki Seki
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Journal Title
Plant and Cell Physiology
Volume: 57
Issue: 4
Pages: 776-783
DOI
Related Report
Peer Reviewed
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