| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Outline of Final Research Achievements |
In this work, we have continued the mechanistic studies of cell death induced by amphiphilic Ir complexes containing cationic peptides. We designed and synthesized new cationic amphiphilic Ir complexes having photoreactive diazirine groups for photoaffinity labeling to identify the target molecules of the Ir complex. A proteomic analysis of the products obtained by the photoirradiation of Ir complex with Jurkat cells suggests that the Ca2+-binding protein “calmodulin (CaM)” is one of target proteins of the Ir complexes. Indeed, cationic amphiphilic Ir complex forms a stable complex with the Ca2+-CaM complex, as evidenced by luminescence titration experiments.
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