| Project/Area Number |
16K18858
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | 生物発光 / 電子移動 / 分子プローブ / プローブ開発 / インビボイメージング |
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| Outline of Final Research Achievements |
The purpose of this study is to establish a molecular design for visualizing biomolecules utilizing firefly bioluminescence, and to apply the bioluminescence probes to in vivo imaging. Specifically, the bioluminescence of the probes is quenched by electron transfer in the excited state, but when target biomolecules exist, the probes can emit light due to chemical change of the quenching moiety. For investigation of the strategy, various test substrates were synthesized and the properties were evaluated. Among them, the luminescence was observed from substrates that are considered to be quenched, and the intensity was quite high. The result may be applied to new strategy for design of highly luminescent substrates.
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