Development of transfection methods with low cell-to-cell variability based on the dispersity
| Project/Area Number |
16K18863
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Physical pharmacy
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| Research Institution | Nagasaki University |
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Principal Investigator |
FUMOTO Shintaro 長崎大学, 医歯薬学総合研究科(薬学系), 准教授 (70380988)
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| Research Collaborator |
PENG Jian Qing
WANG Shu
KOU Tomotaka
NOCHISE Nobuhiro
HIROSE Saori
TANAKA Katsuya
SHIRAISHI Aki
NAKAMURA Koji
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
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| Keywords | 遺伝子・核酸工学材料 / 遺伝子導入機構 / エンドサイトーシス / 細胞間差 / リポソーム / ナノ材料 / 薬学 / 薬剤学 / ドラッグデリバリーシステム / 遺伝子治療 |
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| Outline of Final Research Achievements |
It is a big challenge to resolve the problem of cell-to-cell variability as well as transfection efficiency in non-viral gene transfer. The aim of this study was to develop the non-viral vector with low cell-to-cell variability by regulation of dispersity of the vector. We formulated syn-insertion of detachable PEGylated lipids to the lipoplex during formation. It was possible to maintain hepatic gene expression with regulation of dispersity of the lipoplex. Furthermore, we sought the suitable materials for this methods. As a result, we succeeded in enhancing gene expression of lipoplex with dispersity regulation.
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Report
(3 results)
Research Products
(12 results)
