| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
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| Outline of Final Research Achievements |
Chronic hepatitis B is a global health problem caused by the hepatitis B virus (HBV) infection, which leads to liver cancer and liver cirrhosis. There are two types of drugs for the treatment of HBV infection: nucleotide analogue and interferon. However, these drugs can hardly eliminate HBV completely from the infected cells. Aim of this study was to obtain structural basis for HBV infection useful for the treatment of HBV infection.
The preS, preS1, and preS2 regions of the surface protein of HBV, which are important for infection, were prepared in large quantities for structural analyses. Assignments of NMR signals derived from preS, preS1, and preS2 were successfully established, which can be used for residue specific analyses of the interaction with NTCP, the HBV receptor specifically expressed in liver. After the examination of some expression vectors, E. coli expression of NTCP was achieved.
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