Discovery of new natural resources and analysis of their metabolites

• Project/Area Number: 16K18896
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Natural medicines
• Research Institution: Hiroshima University
• Principal Investigator: yamano yoshi 広島大学, 医系科学研究科(薬), 助教 (70650597)
• Research Collaborator: matsunami katsuyoshi ; Sugimoto sachiko
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2018: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000); Fiscal Year 2016: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 難培養性微生物 / 探索研究 / 構造解析 / 生物活性物質 / 天然資源 / 天然物

Outline of Final Research Achievements

In this study, we developed a culture device named generalized isolation chip (gi-chip) in order to cultivate uncultured microorganisms. gi-chip is an instrument capable of separating and culturing microorganisms in a natural environment. By using gi-chip, it has been possible to obtain a large number of new microorganisms with high potential. In addition, two known compounds and one new compound were isolated as anti-leishmania substances from the obtained microorganism.

Academic Significance and Societal Importance of the Research Achievements

本研究を行ったことにより、新種の可能性が高い多数の微生物を分離培養することができる新しい培養器具が完成した。また、それら微生物から新薬の候補となる可能性をもつ化合物を精製し、構造解析した。
本研究で作成した培養器具は安価に作成することができ、さらに一般的な研究室にある実験器具だけで利用可能であることから汎用性が高く、新種微生物の分離培養を広く普及させられる可能性を有している。今後、本培養器具により、これまでに利用することができなかった多数の新種微生物が利用できるようになり、それら微生物が作り出す化合物や、たんぱく質、ゲノム情報などは薬の開発や他の研究分野の発展に寄与することが期待できる。

Research Products

• [Journal Article] Nematicidal compounds of Peperomia japonica. (2018)
  • Author(s): Nagashima, Kazumi; Yamano, Yoshi; Sugimoto, Sachiko; Ishiwata, Kenji; Kanuka, Hirotaka; Otsuka, Hideaki; Matsunami, Katsuyoshi.
  • Journal Title: Phytochemistry Letters Volume: 27 Pages: 30-35
  • DOI: 10.1016/j.phytol.2018.06.015
  • Peer Reviewed
• [Journal Article] Phenolic compounds from the leaves of Breynia officinalis and their tyrosinase and melanogenesis inhibitory activities (2017)
  • Author(s): Sasaki Ayano、Yamano Yoshi、Sugimoto Sachiko、Otsuka Hideaki、Matsunami Katsuyoshi、Shinzato Takakazu
  • Journal Title: Journal of Natural Medicines Volume: 72 Issue: 2 Pages: 381-389
  • DOI: 10.1007/s11418-017-1148-8
  • NAID: 40021511260
  • Peer Reviewed
• [Journal Article] Chemical structures of constituents from the leaves of Polyscias balfouriana. (2017)
  • Author(s): Sugimoto, Sachiko; Yoshi Yamano; Khalil, Hany Ezat; Otsuka, Hideaki; Kamel, Mohamed Salah; Matsunami, Katsuyoshi
  • Journal Title: Journal of Natural Medicine Volume: 71 Issue: 3 Pages: 558-563
  • DOI: 10.1007/s11418-017-1081-x
  • NAID: 40021218383
  • Peer Reviewed / Open Access / Int'l Joint Research