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Mechanism of SOX2 expression through G-quadruplex in cancer stem cells

Research Project

Project/Area Number 16K18908
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Drug development chemistry
Research InstitutionChiba University

Principal Investigator

Iida Keisuke  千葉大学, 大学院理学研究院, 助教 (70719773)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsSOX2 / AXL / がん / 細胞弾性 / グアニン四重鎖 / がん幹細胞 / G4リガンド / 発がん二段階実験
Outline of Final Research Achievements

SOX2 are thought to contribute to tumorigenesis in squamous cell carcinoma. We monitored Sox2 expression in the epidermis of mice treated with DMBA/TPA or DMBA/OA. As a results, SOX2 expression is absent in the epidermis of control mice but appears in epidermal hyperplasia during chemical-induced carcinogenesis. Now, optimize condition of culture for SOX2 positive cells are currently underway.
Moreover, we found AXL, tyrosine kinase receptor as factor of malignant progression in non-small cell lung cancer cells. Phosphorylation of AXL stimulates cellular softening and motility. Knockdown of AXL led to cellular stiffening and decrease of motility. Now we are trying to repress of AXL expression by small molecules.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (18 results)

All 2018 2017 2016

All Journal Article (11 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 11 results,  Open Access: 4 results,  Acknowledgement Compliant: 2 results) Presentation (7 results) (of which Invited: 1 results)

  • [Journal Article] Down-regulation of histone deacetylase 4, -5 and -6 as a mechanism of synergistic enhancement of apoptosis in human lung cancer cells treated with the combination of a synthetic retinoid, Am80 and green tea catechin2017

    • Author(s)
      Oya Yukiko、Mondal Anupom、Rawangkan Anchalee、Umsumarng Sonthaya、Iida Keisuke、Watanabe Tatsuro、Kanno Miki、Suzuki Kaori、Li Zhenghao、Kagechika Hiroyuki、Shudo Koichi、Fujiki Hirota、Suganuma Masami
    • Journal Title

      Journal of Nutritional Biochemistry

      Volume: 42 Pages: 7-16

    • DOI

      10.1016/j.jnutbio.2016.12.015

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Targeting glioma stem cells in vivo by a G-quadruplex-stabilizing synthetic macrocyclic hexaoxazole2017

    • Author(s)
      Nakamura Takahiro、Okabe Sachiko、Yoshida Haruka、Iida Keisuke、Ma Yue、Sasaki Shogo、Yamori Takao、Shin-ya Kazuo、Nakano Ichiro、Nagasawa Kazuo、Seimiya Hiroyuki
    • Journal Title

      Scientific Reports

      Volume: 7 Issue: 1 Pages: 3605-3605

    • DOI

      10.1038/s41598-017-03785-8

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Structure-Dependent Binding of hnRNPA1 to Telomere RNA2017

    • Author(s)
      Liu Xiao、Ishizuka Takumi、Bao Hong-Liang、Wada Kei、Takeda Yuma、Iida Keisuke、Nagasawa Kazuo、Yang Danzhou、Xu Yan
    • Journal Title

      Journal of the American Chemical Society

      Volume: 139 Issue: 22 Pages: 7533-7539

    • DOI

      10.1021/jacs.7b01599

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Cell softening in malignant progression of human lung cancer cells by activation of receptor tyrosine kinase AXL2017

    • Author(s)
      Iida K, Sakai R, Yokoyama S, Kobayashi N, Togo S , Yoshikawa HY, Rawangkan A, Namiki K, Suganuma M.
    • Journal Title

      Sci. Rep.,

      Volume: 7 Issue: 1 Pages: 1-11

    • DOI

      10.1038/s41598-017-18120-4

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] A single-round selection of selective DNA aptamers for mammalian cells by polymer-enhanced capillary transient isotachophoresis2017

    • Author(s)
      Kazuki Hirose, Maho Tsuchida, Hinako Asakura, Koji Wakui, Keitaro Yoshimoto, Keisuke Iida, Makoto Sato, Masami Shibukawa, Masami Suganuma, Shingo Saito
    • Journal Title

      Analyst

      Volume: 142 Issue: 21 Pages: 4030-4038

    • DOI

      10.1039/c7an00909g

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Phorbol esters in seed oil of Jatropha curcas L. (saboodam in Thai) and their association with cancer prevention: from the initial investigation to the present topics2017

    • Author(s)
      Fujiki Hirota、Suttajit Maitree、Rawangkan Anchalee、Iida Keisuke、Limtrakul Pornngarm、Umsumarng Sonthaya、Suganuma Masami
    • Journal Title

      Journal of Cancer Research and Clinical Oncology

      Volume: 143 Issue: 8 Pages: 1359-1369

    • DOI

      10.1007/s00432-017-2341-6

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Design and synthesis of unsymmetric macrocyclic hexaoxazoles compounds with ability to induce distinct G-quadruplex topologies in telomeric DNA2016

    • Author(s)
      Mai Sakuma, Yue Ma, Yamato Tsushima, Keisuke Iida, Takatsugu Hirokawa, Kazuo Nagasawa
    • Journal Title

      Org. Biomol. Chem.

      Volume: 14 Issue: 22 Pages: 5109-5116

    • DOI

      10.1039/c6ob00437g

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Journal Article] Biophysical Approach to Mechanisms of Cancer Prevention and Treatment with Green Tea Catechins2016

    • Author(s)
      Masami Suganuma, Atsushi Takahashi, Tatsuro Watanabe, Keisuke Iida,Takahisa Matsuzaki, Hiroshi Y. Yoshikawa, Hirota Fujiki
    • Journal Title

      Molecules

      Volume: 21 Issue: 11 Pages: 5166-5166

    • DOI

      10.3390/molecules21111566

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Structural regulation by a G-quadruplex ligand increases binding abilities of G-quadruplex-forming aptamers2016

    • Author(s)
      Kaori Tsukakoshi, Yuri Ikuta, Koichi Abe, Wataru Yoshida, Keisuke Iida, Yue Ma, Kazuo Nagasawa, Koji Sode, Kazunori Ikebukuro
    • Journal Title

      Chem. Commun.

      Volume: 52 Issue: 85 Pages: 12646-12649

    • DOI

      10.1039/c6cc07552e

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] Detection of DNA methylation of G-quadruplex and i-motif-forming sequences by measuring the initial elongation efficiency of PCR2016

    • Author(s)
      Wataru Yoshida, Hitomi Yoshioka, Daniyah Habiballah Bay, Keisuke Iida, Kazunori Ikebukuro, Kazuo Nagasawa, Isao Karube
    • Journal Title

      Anal. Chem.

      Volume: 88 Issue: 14 Pages: 7101-7107

    • DOI

      10.1021/acs.analchem.6b00982

    • Related Report
      2016 Research-status Report
    • Peer Reviewed
  • [Journal Article] A Single Molecules Study of a Fluorescently Labeled Telomestatin Derivative and G-Quadruplex Interactions2016

    • Author(s)
      Parastoo Maleki, Yue Ma, Keisuke Iida, Kazuo Nagasawa, Hamza Balci
    • Journal Title

      Nucl. Acids Res.

      Volume: 45 Issue: 1 Pages: 288-295

    • DOI

      10.1093/nar/gkw1090

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] 光学活性アミノイミノフェノール-銅錯体を用いるエナンチオ選択的 3-ベンゾイルオキシピロロインドリン化合物の合成2018

    • Author(s)
      牧野 宏輝、飯田 圭介、荒井 孝義
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] グアニン四重鎖と相互作用する大環状オキサゾール型蛍光リガンドの創製2018

    • Author(s)
      馬 悦、飯田 圭介、長澤 和夫
    • Organizer
      日本化学会第98春季年会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 4本鎖DNAと結合する化合物の合成と評価2017

    • Author(s)
      飯田 圭介
    • Organizer
      有機合成化学協会関東支部若手研究者のためのセミナー
    • Related Report
      2017 Annual Research Report
    • Invited
  • [Presentation] 定量PCR法を用いた四重鎖中のメチル化DNA検出法の開発2016

    • Author(s)
      吉岡仁美, 飯田圭介, 長澤和夫, 池袋一典, 軽部征夫, 吉田亘
    • Organizer
      第39回日本分子生物学会大会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report
  • [Presentation] 高分子増強過渡的等速電気泳動法を用いるヒト非小細胞肺がん株PC‐9結合型DNAアプタマーの選抜2016

    • Author(s)
      土田真帆, 廣瀬和生, 朝倉妃奈子, 飯田圭介, 佐藤誠, 吉本敬太郎, 菅沼雅美, 渋川雅美, 齋藤伸吾
    • Organizer
      第36回キャピラリー電気泳動シンポジウム
    • Place of Presentation
      徳島県 徳島市 徳島大学 常三島キャンパス
    • Year and Date
      2016-11-09
    • Related Report
      2016 Research-status Report
  • [Presentation] 受容体型チロシンキナーゼAXLの活性化はヒト非小細胞肺がんにおいて 細胞弾性の低下とともに転移性の促進をもたらす2016

    • Author(s)
      飯田圭介、酒井瞭、ソンタヤアムサムアーン、 アンチェリーラワンガーン、菅沼雅美
    • Organizer
      第75回日本癌学会学術総会
    • Place of Presentation
      神奈川県 横浜市 パシフィコ横浜
    • Year and Date
      2016-10-06
    • Related Report
      2016 Research-status Report
  • [Presentation] 緑茶カテキンの細胞硬化を介した転移抑制機構における受容体型チロシンキナーゼAXL抑制の重要性2016

    • Author(s)
      酒井瞭, 飯田圭介, ラワンカーン アンチェリー, 菅沼雅美
    • Organizer
      第89回日本生化学会大会
    • Place of Presentation
      宮城県 仙台市 仙台国際センター、東北大学川内北キャンパス
    • Year and Date
      2016-09-25
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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