| Project/Area Number |
16K18924
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Environmental and hygienic pharmacy
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| Research Institution | Shibaura Institute of Technology |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SUHARA Yoshitomo 芝浦工業大学, システム理工学部・生命科学科, 教授 (30297171)
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| Research Collaborator |
OKANO Toshio 神戸薬科大学, 薬学部, 教授 (20131542)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | ビタミンK / 側鎖切断酵素 / 2',3'-PKH2 / 代謝機構 / UBIAD1 / MK-4 / MD / 遺伝子改変 / 小腸 / オミクス解析 / 遺伝子改変動物 / 腸 |
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| Outline of Final Research Achievements |
In humans and mice, ingested PK is MD which has a side chain cleaved in the small intestine and has no side chains and is converted into MK-4 by UBIAD1 in each tissue. Vitamin K side chain cleavage mechanism has not been elucidated so far and search of side chain cleavage enzymes is also difficult. So we elucidated the in vivo metabolic mechanism of 2',3'-PKH2. As a result, it was found that 2',3'-PKH2 is a vitamin K derivative not undergoing a side chain cleavage reaction, since MD which is an intermediate of the MK-4 conversion reaction was not detected at all in the lymph fluid .
From the results of this study, it is considered that the vitamin K side chain cleavage enzyme selectively recognizes and cleaves double bonds at the 2nd and 3rd positions present in the side chain of PK. If vitamin K side chain cleavage enzyme can be identified in future, it is clarified what kind of mechanism the ingested vitamin K is metabolically absorbed and exercises physiological function.
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