| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
|
|---|
| Outline of Final Research Achievements |
(1) The permeation assay of AQP0 was used the stable L-cell line expressing AQP0. As the result, AQP0 can permeate ascorbic acid ex vivo, and also indicate that there is a difference between the import and export of ascorbic acid via AQP0 channel.
(2) The cell adhesion assay of AQP0 was revealed that AQP0 could bind directly to the opposing membrane through the C-loop domain. We also found that 109Pro and 110Pro were important amino acids for cell adhesion, but mutations in the C-loop of AQP0 did not affect AQP0 water permeability.
|
