| Project/Area Number |
16K18975
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | The University of Tokyo |
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Principal Investigator |
KIDO Taketomo 東京大学, 分子細胞生物学研究所, 助教 (00401034)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | 肝臓 / 肝細胞 / 類洞内皮細胞 / 星細胞 / iPS細胞 / 細胞・組織 |
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| Outline of Final Research Achievements |
During liver development, liver progenitor cells (LPCs) differentiate into mature hepatocytes through interactions with hepatic non-parenchymal cells (NPCs) such as liver sinusoidal endothelial cells (LSECs) and hepatic stellate cells (HSCs). In this study, to induce functional hepatocytes from hiPSCs, we established a co-culture system of LPCs and NPCs. We show that TGFb and Rho signaling pathways, respectively, regulate the proliferation and maturation of LSECs and HSCs isolated from mouse fetal livers. Based on the results in mice, we established the hiPSC-derived LSECs and HSCs. They could support proliferation and maturation of hiPSC-derived LPCs by expressing numerous hepatic mitogens and extracellular matrix proteins.
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