| Project/Area Number |
16K18984
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General anatomy (including histology/embryology)
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| Research Institution | Hyogo Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
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| Keywords | GnRHニューロン / 炎症性サイトカイン / 視床下部 / GnRH / IL-18 / 解剖学 |
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| Outline of Final Research Achievements |
We focused on the relationship between inflammatory cytokines and neuroendocrine cells, and identified IL-18Rα-expressing and IL-18-expressing cells in the male mouse and rat forebrain. IL-18Rα was expressed in approximately 60 % of GnRH-immunopositive perikarya, and IL-18 was distributed in all GnRH-immunopositive perikarya. We next confirmed the expression of IL-18 and its receptor in GT1-7 cells, a GnRH-producing cell line. We also detected the expression of other inflammatory cytokines, TNF-α, IL-1β and IL-6, and their receptors in GT1-7 cells. Our observations indicate that these inflammatory cytokines may exert direct effects upon GnRH neurons via their specific receptors. There is a possibility that these cytokines regulate the function of GnRH neurons in an autocrine or paracrine manner.
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