| Project/Area Number |
16K19000
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
General physiology
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| Research Institution | National Institute for Physiological Sciences |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
|
| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | イオンチャネル / Na+チャネル / Two-pore channel / TPC / Nav / 膜電位依存性 / 光生理学 / ホスホイノシチド / 膜電位存性Na+チャネル / 電気生理学 / Two-Pore channel / 膜電位依存性イオンチャネル / 膜タンパク質 / 光生理学的解析 / Two-pore Na+ channel / voltage-gated channel / 生体分子 |
|---|
| Outline of Final Research Achievements |
We analyzed the Two-pore Na+ channel 3 (XtTPC3) to reveal the different roles of two voltage sensor domains using the electrophysiological technique. We found that the domain-II contributes to the voltage-dependence more than domain-I in XtTPC3. In addition, the unique property of XtTPC3, in which long and high voltage stimulus gradually increases the current amplitudes of XtTPC3, was found to be dependent on a type of phosphoinositide. Furthermore, we revealed that the positively charged cluster in S4/S5 linker and helix S6 of domain-I is indispensable for the phosphoinositide dependence of XtTPC3.
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