Identification of neural populations projecting kisspeptin neurons in rat brain

• Project/Area Number: 16K19010
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Environmental physiology(including physical medicine and nutritional physiology)
• Research Institution: Okayama University of Science (2017); Nippon Medical School (2016)
• Principal Investigator: Takumi Ken 岡山理科大学, 理学部, 准教授 (40553269)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: キスペプチン / 視床下部 / 神経回路 / 脳・神経

Outline of Final Research Achievements

The aim of this study was to identify the neuronal populations projecting kisspeptin neurons which regulate reproductive functions in mammals. Although injection of WGA to the arcuate nucleus resulted in labeling neurons in several contralateral nuclei, it is difficult to identify the neuronal populations since injection site was not limited. Dual-immunohistochemistry revealed that VGAT immunopositive puncta and VGluT2 immunopositive puncta were closely apposed to kisspeptin neurons. This suggests that GABAergic and Glutamatergic neurons are a part of neuronal populations which project and regulate kisspeptin neurons.

Research Products

• [Journal Article] Morphological Analysis of Trafficking and Processing of Anionic and Cationic Liposomes in Cultured Cells (2018)
  • Author(s): Tomori Y, Iijima N, Hinuma S, Ishii H, Takumi K, Takai S, Ozawa H
  • Journal Title: ACTA HISTOCHEMICA ET CYTOCHEMICA Volume: 51 Issue: 2 Pages: 81-92
  • DOI: 10.1267/ahc.17021
  • NAID: 130006727980
  • ISSN: 0044-5991, 1347-5800
• [Journal Article] Development of an imaging system for in vivo real-time monitoring of neuronal activity in deep brain of free-moving rats. (2017)
  • Author(s): Iijima N, Miyamoto S, Matsumoto K, Takumi K, Ueta Y, Ozawa H.
  • Journal Title: Histochem Cell Biol. Volume: 148 Issue: 3 Pages: 289-298
  • DOI: 10.1007/s00418-017-1576-2
  • Peer Reviewed / Open Access
• [Journal Article] Kisspeptin expression is decreased in the arcuate nucleus of hypothyroid female rats with irregular estrus cycles (2017)
  • Author(s): Tomori Y, Takumi K, Iijima N, Takai S, Ozawa H.
  • Journal Title: Neuroscience Research Volume: 117 Pages: 35-41
  • DOI: 10.1016/j.neures.2016.11.005
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Presentation] エストロゲン受容体サブタイプ（α, β）のキスペプチン発現調節における役割の解明 (2017)
  • Author(s): 託見健, 金谷萌子, 佐越祥子, 永田和代, 小川園子, 小澤一史
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] 性成熟に伴う正中隆起の星状グリア細胞の形態変化及びGnRHニューロン軸索終末との連関 (2017)
  • Author(s): 楊春英, 託見健, 小澤一史
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] 甲状腺機能低下が視床下部におけるキスペプチン発現に及ぼす影響 (2017)
  • Author(s): 友利裕二, 託見健, 飯島典生, 小澤一史
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28
• [Presentation] 日本医科大学における実習手順 -全体のデザイン、モチベーション、評価- (2017)
  • Author(s): 飯島典生, 託見健, 石井寛高, 岩田衣世, 楊春英, 肥後心平, 小澤一史
  • Organizer: 第122回日本解剖学会総会・全国学術集会
  • Place of Presentation: 長崎大学坂本キャンパス
  • Year and Date: 2017-03-28