Functional analysis of long noncoding RNAs that regulate epithelial-mesenchymal transition

• Project/Area Number: 16K19030
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Kanazawa University
• Principal Investigator: Terashima Minoru 金沢大学, がん進展制御研究所, 助教 (80507434)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 上皮間葉転換 / EMT / 長鎖非コードRNA / lncRNA / ポリコーム抑制複合体 / PRC2 / ヒストンメチル化

Outline of Final Research Achievements

We have focused on epithelial-mesenchymal transition (EMT) as a mechanism of cancer progression and reported that polycomb repressive complex 2 (PRC2), which is one of epigenetic regulators, induces EMT by repressing the expression of epithelial genes. In this study, we proposed that MEG3 long noncoding RNA guides PRC2 to its target gene loci. These results contribute to understanding of long noncoding RNA involved in cancer progression.

Research Products

• [Journal Article] Epigenetic regulation of epithelial-mesenchymal transition by KDM6A histone demethylase in lung cancer cells. (2017)
  • Author(s): Terashima M, Ishimura A, Wanna-Udom S and Suzuki T
  • Journal Title: Biochem. Biophys. Res. Commun. Volume: 490 Issue: 4 Pages: 1407-13
  • DOI: 10.1016/j.bbrc.2017.07.048
  • Peer Reviewed / Open Access
• [Journal Article] MEG3 long noncoding RNA contributes to the epigenetic regulation of epithelial-mesenchymal transition in lung cancer cell lines. (2017)
  • Author(s): Terashima M, Tange S, Ishimura A and Suzuki T.
  • Journal Title: J Biol. Chem. Volume: 292(1) Issue: 1 Pages: 82-99
  • DOI: 10.1074/jbc.m116.750950
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] DOT1L histone methyltransferase regulates the expression of BCAT1 and is involved in sphere formation and cell migration of breast cancer cell lines. (2016)
  • Author(s): Oktyabri D, Ishimura A, Tange S, Terashima M, Suzuki T.
  • Journal Title: Biochimie Volume: 123 Pages: 20-31
  • DOI: 10.1016/j.biochi.2016.01.005
  • Peer Reviewed / Open Access
• [Presentation] TGF-βによって誘導される上皮間葉転換のエピジェネティクス制御機構 (2017)
  • Author(s): 寺島 農、 丹下 正一朗、Sasithorn Wanna-udom、石村 昭彦、鈴木 健之
  • Organizer: 2017年度 生命科学系学会合同年次大会
• [Presentation] MEG3長鎖ノンコーディングRNAによる上皮間葉転換のエピジェネティック制御機構 (2017)
  • Author(s): 寺島 農
  • Organizer: HOKURIKU RNA CLUB
• [Presentation] Epigenetic Regulation of Epithelial-Mesenchymal Transition in Cancer Cells. (2017)
  • Author(s): Terashima M, Ishimura A, Tange S and Suzuki T.
  • Organizer: The 12th International Symposium of Institute Network
  • Int'l Joint Research
• [Presentation] 上皮間葉転換におけるPRC2遺伝子発現抑制メカニズム (2016)
  • Author(s): Terashima M, Tange S, Ishimura A and Suzuki T.
  • Organizer: 第39回日本分子生物学会年会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2016-11-30
• [Presentation] 上皮間葉転換におけるポリコーム抑制複合体PRC2を介したエピジェネティック制御機構 (2016)
  • Author(s): Minoru Terashima
  • Organizer: 第3回北陸エピジェネティクス研究会
  • Place of Presentation: 福井AOSSA
  • Year and Date: 2016-11-21
• [Remarks] 金沢大学がん進展制御研究所 機能ゲノミクス分野
  • URL: http://ganken.cri.kanazawa-u.ac.jp/Genomics/index.html