Growth cone reformation in the axon regeneration

• Project/Area Number: 16K19032
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General medical chemistry
• Research Institution: Nagoya University
• Principal Investigator: li chun 名古屋大学, 高等研究院(理), 特任助教 (40755716)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2018: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000); Fiscal Year 2016: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
• Keywords: 神経再生 / 線虫C.elegans / 神経軸索再生 / 神経軸索 / C.elegans / JNK経路 / 成長円錐

Outline of Final Research Achievements

1.We found that LET-502-MLC-4-NMY-2 pathway regulated growth cone reformation in the axon regeneration. 2.We found that SVH-16, a CDK type protein kinase might have function in the upstream of JNK pathway or parallel to JNK pathway. 3. We found that C.elegans homolog ATN-1, actinin alpha 1 can bind to ALP-1, an ortholog of human LDB3 (LIM domain binding 3), which had important function in the axon regeneration.

Academic Significance and Societal Importance of the Research Achievements

神経軸索の再生機構の解明は、医学的には事故や疾患による神経切断や欠損の治療法を開発する上で重要であり、社会的にも喫緊の研究課題である。従って、本研究は単なる学術的発見にとどまらず、ヒトにおける神経再生誘導の理解および再生治療に繋がることが期待される点で、日本発の極めて重要な研究と考える。

Research Products

• [Journal Article] UNC-16/JIP3 regulates early events in synaptic vesicle protein trafficking via LRK-1/LRRK2 and AP complexes (2017)
  • Author(s): Choudhary Bikash、Kamak Madhushree、Ratnakaran Neena、Kumar Jitendra、Awasthi Anjali、Li Chun、Nguyen Ken、Matsumoto Kunihiro、Hisamoto Naoki、Koushika Sandhya P.
  • Journal Title: PLOS Genetics Volume: 13 Issue: 11 Pages: 1-25
  • DOI: 10.1371/journal.pgen.1007100
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Chaperone complex BAG2-HSC70 regulates localization of Caenorhabditis elegans leucine-rich repeat kinase LRK-1 to the Golgi (2016)
  • Author(s): Fukuzono T, Pastuhov SI, Fukushima O1, Li C, Hattori A, Iemura SI, Natsume T, Shibuya H, Hanafusa H, Matsumoto K, Hisamoto N.
  • Journal Title: Genes Cells． Volume: 21(4) Issue: 4 Pages: 311-24
  • DOI: 10.1111/gtc.12338
  • Peer Reviewed / Open Access
• [Presentation] Axon regeneration is regulated by Ets-C/EBP transcription complexes generated by activation of the cAMP/Ca2+ signaling pathways (2016)
  • Author(s): chun li
  • Organizer: 7th Asia-Pacific C.elegans Meeting
  • Place of Presentation: Beijing Friendship Hotel
  • Year and Date: 2016-06-25
  • Int'l Joint Research