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Regulatory mechanisms of endothelial-specific Tmem100 expression during cardiovascular development

Research Project

Project/Area Number 16K19044
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionNational Cardiovascular Center Research Institute

Principal Investigator

Kinugasa-Katayama Yumi  国立研究開発法人国立循環器病研究センター, 研究所, 流動研究員 (20570675)

Research Collaborator NAKAGAWA OSAMU  
WATANABE YUSUKE  
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
KeywordsTmem100 / 血管形成 / 血管内皮 / 胎生期 / 胎生期心血管 / 遺伝子発現制御 / 発生・分化 / 発現制御
Outline of Final Research Achievements

We previously demonstrated that the expression of transmembrane protein No.100 (TMEM100) was markedly induced by the activation of ALK1 signaling in cultured human endothelial cells. Tmem100 was expressed in arterial endothelial cells during mouse embryo development, and Tmem100 null mice showed embryonic lethality due to impairment of endothelial differentiation and vascular morphogenesis. However, the regulatory mechanisms of TMEM100 expression in embryonic vasculature are largely unknown. We identified a well-conserved endothelial enhancer of mouse Tmem100/human TMEM100 through BAC-based reporter analysis in mouse embryos. The reporter expression pattern was restricted to intermediate to large arteries and distinct from broader expression of the Alk1 knock-in LacZ reporter in the smaller caliber-vessels. These results suggest that the TMEM100 expression in embryonic endothelial cells is likely to be controlled by a unique combination of BMP-ALK1 and other signaling pathways.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (6 results)

All 2018 2017 2016

All Journal Article (2 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 2 results,  Open Access: 1 results) Presentation (4 results)

  • [Journal Article] Serum/glucocorticoid-regulated kinase 1 as a novel transcriptional target of bone morphogenetic protein-ALK1 receptor signaling in vascular endothelial cells.2018

    • Author(s)
      Araki M, Hisamitsu T, Kinugasa-Katayama Y, Tanaka T, Harada Y, Nakao S, Hanada S, Ishii S, Fujita M, Kawamura T, Saito Y, Nishiyama K, Watanabe Y, Nakagawa O.
    • Journal Title

      Angiogenesis

      Volume: 21 Issue: 2 Pages: 415-423

    • DOI

      10.1007/s10456-018-9605-x

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Cancer cells induce metastasis-supporting neutrophil extracellular DNA traps2016

    • Author(s)
      Park J, Wysocki RW, Amoozgar Z, Maiorino L, Fein MR, Jorns J, Schott AF, Kinugasa-Katayama Y, Lee Y, Won NH, Nakasone ES, Hearn SA, Küttner V, Qiu J, Almeida AS, Perurena N, Kessenbrock K, Goldberg MS, Egeblad M.
    • Journal Title

      Science Translational Medicine

      Volume: 8 Issue: 361

    • DOI

      10.1126/scitranslmed.aag1711

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] 胎生期血管内皮遺伝子Tmem100の心血管形態形成における発現制御機構の解析2017

    • Author(s)
      片山由美、冨松彩佳、渡邉裕介、瀬谷大貴、荒井勇二、磯本祥恵、斎藤能彦、中川修
    • Organizer
      2017年度生命科学系学会合同年次大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 胎生期血管内皮遺伝子Tmem100の心血管形成における発現制御機構2017

    • Author(s)
      片山由美、冨松彩佳、渡邉裕介、久光隆、瀬谷大貴、荒井勇二、磯本祥恵、斎藤能彦、中川修
    • Organizer
      第25回日本血管生物医学会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 胎生期血管内皮遺伝子TMEM100の心血管形態形成における発現制御機構の解析2016

    • Author(s)
      片山由美、渡邉裕介、冨松彩佳、佐藤玄基、岩田裕子、荒井勇二、斎藤能彦、中川修
    • Organizer
      第24回日本血管生物医学会学術集会/第14回日韓血管生物合同シンポジウム
    • Place of Presentation
      長崎市
    • Year and Date
      2016-12-08
    • Related Report
      2016 Research-status Report
  • [Presentation] 胎生期血管内皮遺伝子Tmem100の心血管形態形成における発現制御機構と分子機能の解析2016

    • Author(s)
      片山 由美、岩田 裕子、冨松 彩佳、渡邉 裕介、斎藤 能彦、中川 修
    • Organizer
      第39回日本分子生物学会年会
    • Place of Presentation
      横浜市
    • Year and Date
      2016-11-30
    • Related Report
      2016 Research-status Report

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Published: 2016-04-21   Modified: 2019-03-29  

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