Functional analysis of BIG3 on triple-negative breast cancer progression

• Project/Area Number: 16K19052
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pathological medical chemistry
• Research Institution: Kindai University (2017); The University of Tokushima (2016)
• Principal Investigator: KIMURA Ryuichiro 近畿大学, 医学部, 助教 (20587323)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: 乳癌 / ゲノム / シグナル伝達 / プロテオーム / 発現制御 / 癌 / 蛋白質

Outline of Final Research Achievements

We identified novel BIG3-interacting factors including microtubule-regulated molecule in ER-negative breast cancer. We further analyzed BIG3-these factors interactions and colocalization by immunoprecipitation and immunostaining. On the other hand, knockdown of BIG3 expression with small-interfering RNA reduced E2F1 expression and cellular growth of ER-negative breast cancer. These results suggest that BIG3 directly regulates cellular survival and growth of ER-negative breast cancer through regulation of microtubule or cell cycle.

Research Products

• [Journal Article] Frequent downregulation of LRRC26 by epigenetic alterations is involved in the malignant progression of triple-negative breast cancer (2018)
  • Author(s): Miyagawa Yoshimasa、Matsushita Yosuke、Suzuki Hiromu、Komatsu Masato、Yoshimaru Tetsuro、Kimura Ryuichiro、Yanai Ayako、Honda Junko、Tangoku Akira、Sasa Mitsunori、Miyoshi Yasuo、Katagiri Toyomasa
  • Journal Title: International Journal of Oncology Volume: － Pages: 4301-4301
  • DOI: 10.3892/ijo.2018.4301
  • Peer Reviewed / Open Access
• [Presentation] ムチン型糖転移酵素GALNT6はLGALS3BPの糖鎖修飾を通じて乳癌発症を制御する。 (2017)
  • Author(s): 木村竜一朗、松尾泰佑、尾野雅哉、吉丸哲郎、小松正人、本田純子、朴在賢、中村祐輔、笹三徳、三好康雄、片桐豊雅
  • Organizer: 第76回日本癌学会学術総会（横浜）
• [Presentation] トリプルネガティブ乳がんの悪性化におけるRHBDL2の役割解析 (2017)
  • Author(s): 奥村和正、松下洋輔、小松正人、木村竜一朗、吉丸哲郎、尾野雅哉、三好康雄、本田純子、笹三徳、丹黒章、片桐豊雅
  • Organizer: 第76回日本癌学会学術総会（横浜）
• [Presentation] 糖転移酵素GALNT6はガレクチン結合タンパク質1を糖鎖修飾し，乳癌発症を制御する (2016)
  • Author(s): 木村竜一朗, 尾野雅哉, 松尾泰佑, 吉丸哲郎, 三好康雄, 笹三徳, 片桐豊雅
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] トリプルネガティブ乳癌で高発現が認められるTNRHP1の発現亢進は癌の悪性度と相関する (2016)
  • Author(s): 奥村和正, 小松正人, 木村竜一朗, 尾野雅哉, 吉丸哲郎, 松下洋輔, 三好康雄, 本田純子, 笹三徳, 丹黒章, 片桐豊雅
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Presentation] BIG3-PHB2相互作用を標的とした前立腺がん治療法の開発の可能性 (2016)
  • Author(s): 瀧亮祐, 吉丸哲郎, 大豆本圭, 松下洋輔, 木村竜一朗, 尾野雅哉, 片桐豊雅
  • Organizer: 第75回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜（神奈川県横浜市）
  • Year and Date: 2016-10-06
• [Remarks] 徳島大学・教育研究者総覧（木村竜一朗）
  • URL: http://pub2.db.tokushima-u.ac.jp/ERD/person/277280/work-ja.html