Alteration of driver gene in hyperplastic polyp-associated gastric cancer
| Project/Area Number |
16K19087
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Nara Medical University |
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Principal Investigator |
Luo Yi 奈良県立医科大学, 医学部, 博士研究員 (30633797)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 胃癌 / ドライバー遺伝子 / ピロリ菌 / 胃がん / ドライバー遺伝子変異 / BRAF / EGF / 胃過形成ポリープ / 早期胃癌 / H. ピロリ菌 / cag A / ヘリコバクター / 遺伝子変異 |
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| Outline of Final Research Achievements |
To assess the early carcinogenesis of stomach, we examined hyperplastic foveolar polyps (HFP). The 22 HFPs contained 3 mild dysplasia, one moderate dysplasia, 3 severe dysplasia, and 2 cancers. Five cases of severe dysplasia and cancer showed papillotubular structure and marked accumulation of 8OHdG. All 5 cases is negative for H. pylori. Intestinal phenotype examined by CDX2, MUC2, CD10 was found only in a small part of the only two cases. In the five cases, mutation in BRAF codon 600 (V:GTG to E:GAG) was observed. The BRAF:V600E is known as a rare mutation in gastric cancer. To investigate the effect of H. pylori infection to BRAF mutation, A375 melanoma cell line carrying BRAF:V600E was transfected with CagA and continuously treated with EGF for 38 weeks. After treatment, A375 cells expressed CDX2. BRAF:V600E was still detected; however, vemurafenib sensitivity was disappeared. These data suggest that H. pylori infection abrogate the advantage of BRAF mutation.
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Report
(3 results)
Research Products
(17 results)
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[Journal Article] Expression of cytosolic malic enzyme (ME1) is associated with disease progression in human oral squamous cell carcinoma2018
Author(s)
Nakashima C, Yamamoto K, Fujiwara-Tani R, Luo Y, Matsushima S, Fujii K, Ohmori H, Sasahira T, Sasaki T, Kitadai Y, Kirita T, Kuniyasu H
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Journal Title
Cancer Sci
Volume: in press
Issue: 6
Pages: 2036-2045
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Pro-metastatic signaling of elaidic acid, a trans fatty acid, is associated with lipid rafts.2018
Author(s)
Kishi S, Fujiwara-Tani R, Luo Y, Kawahara I, Goto K, Fujii K, Ohmori H, Nakashima C, Sasaki T, Kuniyasu H
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Journal Title
Oncol Lett
Volume: 15
Pages: 4423-4426
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research
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[Journal Article] Pro-metastatic intracellular signaling of elaidic acid, a trans fatty acid.2017
Author(s)
Fujii K, Luo Y, Nishiguchi Y, Fujiwara R, Kishi S, He S, Yang S, Sasaki T, Ohmori H, Kuniyasu H
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Journal Title
Int J Oncol
Volume: 50
Issue: 1
Pages: 85-92
DOI
Related Report
Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
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