| Budget Amount *help |
¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000)
Fiscal Year 2017: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
|
|---|
| Outline of Final Research Achievements |
To assess the early carcinogenesis of stomach, we examined hyperplastic foveolar polyps (HFP). The 22 HFPs contained 3 mild dysplasia, one moderate dysplasia, 3 severe dysplasia, and 2 cancers. Five cases of severe dysplasia and cancer showed papillotubular structure and marked accumulation of 8OHdG. All 5 cases is negative for H. pylori. Intestinal phenotype examined by CDX2, MUC2, CD10 was found only in a small part of the only two cases. In the five cases, mutation in BRAF codon 600 (V:GTG to E:GAG) was observed. The BRAF:V600E is known as a rare mutation in gastric cancer. To investigate the effect of H. pylori infection to BRAF mutation, A375 melanoma cell line carrying BRAF:V600E was transfected with CagA and continuously treated with EGF for 38 weeks. After treatment, A375 cells expressed CDX2. BRAF:V600E was still detected; however, vemurafenib sensitivity was disappeared. These data suggest that H. pylori infection abrogate the advantage of BRAF mutation.
|
