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Microbial population analysis of vector arthropods for developing new control method of vector borne diseases

Research Project

Project/Area Number 16K19112
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Parasitology (including sanitary zoology)
Research InstitutionHokkaido University

Principal Investigator

Qiu Yongjin  北海道大学, 人獣共通感染症リサーチセンター, 博士研究員 (00760985)

Project Period (FY) 2016-04-01 – 2019-03-31
Project Status Completed (Fiscal Year 2018)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥1,300,000 (Direct Cost: ¥1,000,000、Indirect Cost: ¥300,000)
Keywordsマダニ / 細菌叢 / アフリカ / ボレリア / ザンビア / ウシジラミ / リケッチア / コクシエラ / 細胞内寄生細菌 / 吸血性節足動物 / 感染症 / 節足動物
Outline of Final Research Achievements

The purpose of this project was to analyze the microbial population in several arthropod species and detect pathogens in Africa. Soft tick, hard tick, and lice were collected in several area in Zambia and subjected to high-throughput sequencing analysis based on 16S ribosomal RNA amplicons. More than 100 different bacterial genera were detected in each sample. In part of the pathogen detection, soft ticks and their blood host (Bats) were used for isolation of Borrelia. We successfully isolated Borrelia sp. from bats. Subsequent analysis revealed that the Borrelia sp. was belonged to New World relapsing fever borrelia even it was detected old world. We designated that new species of Borrelia as Candidatus Borrelia fainii.

Academic Significance and Societal Importance of the Research Achievements

本研究では、マダニ類を中心に19種258サンプルの節足動物の細菌叢を解析し、アフリカにおける節足動物が保有する細菌叢の情報基盤を構築した。節足動物の共生細菌を利用し感染症の制御方法は現在注目を浴びている手法である。細菌叢解析で得られた知見は、この新しい制御方法を開発するのに重要な情報をもたらす。また、本研究では新種の新世界型回帰熱ボレリアの分離に成功した。本菌は、アフリカ大陸(旧世界)で分離された唯一の新世界型回帰熱ボレリアである。本菌をより詳細に解析することでBorrelia属細菌の分布・進化・系統分類に関する重要な知見が得られることが期待される。

Report

(4 results)
  • 2018 Annual Research Report   Final Research Report ( PDF )
  • 2017 Research-status Report
  • 2016 Research-status Report
  • Research Products

    (7 results)

All 2019 2018 2017 Other

All Int'l Joint Research (1 results) Journal Article (3 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 3 results,  Open Access: 1 results) Presentation (3 results) (of which Int'l Joint Research: 3 results,  Invited: 1 results)

  • [Int'l Joint Research] The University of Zambia(ザンビア)

    • Related Report
      2018 Annual Research Report
  • [Journal Article] Viral population analysis of the taiga tick, <i>Ixodes persulcatus,</i> by using Batch Learning Self-Organizing Maps and BLAST search2019

    • Author(s)
      QIU Yongjin、ABE Takashi、NAKAO Ryo、SATOH Kenro、SUGIMOTO Chihiro
    • Journal Title

      Journal of Veterinary Medical Science

      Volume: 81 Issue: 3 Pages: 401-410

    • DOI

      10.1292/jvms.18-0483

    • NAID

      130007617731

    • ISSN
      0916-7250, 1347-7439
    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Human Borreliosis Caused by a New World Relapsing Fever Borrelia-like Organism in the Old World2018

    • Author(s)
      Qiu Yongjin、Nakao Ryo、Hang’ombe Bernard Mudenda、Sato Kozue、Kajihara Masahiro、Kanchela Sharon、Changula Katendi、Eto Yoshiki、Ndebe Joseph、Sasaki Michihito、Thu May June、Takada Ayato、Sawa Hirohumi、Sugimoto Chihiro、Kawabata Hiroki
    • Journal Title

      Clinical Infectious Diseases

      Volume: 印刷中 Issue: 1 Pages: 107-112

    • DOI

      10.1093/cid/ciy850

    • Related Report
      2018 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Tick-borne haemoparasites and Anaplasmataceae in domestic dogs in Zambia.2018

    • Author(s)
      Yongjin Qiu, Chiho Kaneko, Masahiro Kajihara, Saasa Ngonda, Edgar Simulundu, Walter Muleya, May Jun Thu, Mudenda Bernard Hang’ombe, Ken Katakura, Ayato Takada, Hirofumi Sawa, Martin Simuunza, Ryo Nakao
    • Journal Title

      Ticks and tick-borne diseases.

      Volume: 印刷中

    • Related Report
      2017 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Presentation] Candidatus Borrelia fainii, a New World relapsing fever Borrelia-like organism, the casetive agent of human boreliosis in Zambia.2018

    • Author(s)
      Yongjin Qiu, Ryo Nakao, Bernard Mudenda Hang’ombe, Kozue Sato, Masahiro Kajihara, Sharon Kanchela, Katendi Changula, Yoshiki Eto, Joseph Ndebe, Michihito Sasaki, May June Thu, Ayato Takada, Hirohumi Sawa, Chihiro Sugimoto, Hiroki Kawabata
    • Organizer
      The 3rd international joint symposium promotion of infectious diseases research cooperation between Africa and japan toward science, technology and innovation
    • Related Report
      2018 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] Detection and characterization of tick-borne bacterial and protozoan pathogens in domestic dogs, cattle, and ticks in Zambia.2017

    • Author(s)
      Yongjin Qiu, Chiho Kaneko, Masahiro Kajihara, Martin Simuunza, Ngonda Saasa, Hayato Furumoto, Joseph Ndebe, Penjani Kapila, May June Thu, Mudenda Bernard Hang’ombe, Ayato Takada, Chihiro Sugimoto, Ryo Nakao
    • Organizer
      2017 Annual Scientific Symposium ~Sustainable Animal and Human Health though Research Under One Health~
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research
  • [Presentation] Investigation of tick-borne bacterial and protozoan pathogens in domestic dogs, cattle, and ticks in Zambia2017

    • Author(s)
      Yongjin Qiu, Chiho Kaneko, Masahiro Kajihara, Martin Simuunza, Ngonda Saasa, Hayato Furumoto, Joseph Ndebe, Penjani Kapila, May June Thu, Mudenda Bernard Hang'ombe, Ayato Takada, Chihiro Sugimoto, Ryo Nakao
    • Organizer
      The 9th International Congress on Ticks and Tick-Borne Pathogens
    • Related Report
      2017 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2020-03-30  

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