| Project/Area Number |
16K19116
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Parasitology (including sanitary zoology)
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| Research Institution | Osaka University |
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Principal Investigator |
Ma Jisu 大阪大学, 微生物病研究所, 助教 (90755266)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | トキソプラズマ / デンスグラニュール / エフェクター機構 / GRA / GRA15 / オルガネラ / エフェクター / 病原性因子 |
|---|
| Outline of Final Research Achievements |
We purified host organelles of Toxoplasma-infected cells by ultracentrifugation and searched novel Toxoplasma-derived molecules and tested whether the effectors are derived from dense granules. Moreover, we searched GRA-dependent modulation of host cell signaling pathways by using ASP5-KO Toxoplasma and analyzed the effect of GRA-dependent suppression and activation of host cell signaling pathways comprehensively and globally by luciferase assay. As a result, we succeeded to identify a novel virulence effector candidate protein called TgGIF, which potentially disrupts host Gate-16-dependent host cell-autonomous immune response to Toxoplasma. We will continue to study the virulent role of TgGIF in Toxoplasma immunoparasitology.
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