Elucidation of the function of group 2 innate lymphoid cells in chronic and severe cases of amebiasis

• Project/Area Number: 16K19118
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including sanitary zoology)
• Research Institution: Nagasaki University
• Principal Investigator: NAKAMURA Risa 長崎大学, 熱帯医学研究所, 助教 (50645801)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000); Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
• Keywords: 赤痢アメーバ症 / グループ２自然リンパ球 / Th2サイトカイン / 寄生虫感染症 / 免疫学 / 腸赤痢アメーバ症 / 感染症 / 寄生虫 / Th2 サイトカイン

Outline of Final Research Achievements

Intestinal amebiasis is chronic and presents severe symptoms such as liver abscess and brain abscess. Preventing the chronic infection of Entamoeba histolytica is important in host defense against severe pathological conditions. It has been reported that intestinal infection of Entamoeba histolytica produces a large amount of Th2 cytokines from the early phase of infection, but its role in the source and pathogenesis and severity has not been clarified. In this study, we found that the source of Th2 cytokines early in infection with Entamoeba histolytica is group 2 innate lymphoid cells (ILC2). Furthermore, the in vivo depletion of ILC2 revealed that ILC2 and its Th2 cytokines contribute to exacerbating the pathogenesis of amebiasis.

Research Products

• [Journal Article] Schistosoma mansoni infection suppresses the growth of Plasmodium yoelii parasites in the liver and reduces gametocyte infectivity to mosquitoes. (2018)
  • Author(s): Moriyasu, T., Nakamura, R., Deloer, S., Senba, M., Kubo, M., Inoue, M., Culleton, R., Hamano, S.
  • Journal Title: PLoS Negl. Trop. Dis. e0006197. Volume: 12 Issue: 1 Pages: e0006197-e0006197
  • DOI: 10.1371/journal.pntd.0006197
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] IL-17A contributes to reducing IFN-gamma/IL-4 ratio and persistence of Entamoeba histolytica during intestinal amebiasis (2017)
  • Author(s): Deloer, S. Nakamura, R. Kikuchi, M. Moriyasu, T. Kalenda, Y. D. J. Mohammed, E. S. Senba, M. Iwakura, Y. Yoshida, H. Hamano, S.
  • Journal Title: Parasitol Int Volume: 66 Issue: 6 Pages: 817-823
  • DOI: 10.1016/j.parint.2017.09.011
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Spatial distribution and risk factors of Schistosoma haematobium and hookworm infections among schoolchildren in Kwale, Kenya. (2017)
  • Author(s): Chadeka EA, Nagi S, Sunahara T, Cheruiyot NB, Bahati F, Ozeki Y, Inoue M, Osada-Oka M, Okabe M, Hirayama Y, Changoma M, Adachi K, Mwende F, Kikuchi M, Nakamura R, Kalenda YDJ, Kaneko S, Hirayama K, Shimada M, Ichinose Y, Njenga SM, Matsumoto S, Hamano S.
  • Journal Title: PLoS Negl Trop Dis Volume: 11 Issue: 9 Pages: e0005872-e0005872
  • DOI: 10.1371/journal.pntd.0005872
  • NAID: 120006987400
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Mouse model of amoebiasis and culture methods of amoeba (2016)
  • Author(s): Deloer S, Nakamura R, Mi-ichi F, Adachi K, Kobayashi S, Hamano S.
  • Journal Title: Parasitol. Int. Volume: in press Issue: 5 Pages: 30044-30047
  • DOI: 10.1016/j.parint.2016.03.012
  • Peer Reviewed / Open Access
• [Presentation] Group 2 innate lymphoid cells exacerbate severe amebic liver abscess by IFN-gamma independent manner in mice. (2018)
  • Author(s): Risa Nakamura, Sharmina Deloer, Masachika Senba, Mihoko Kikuchi, Shigeo Koyasu, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第87回日本寄生虫学会大会
• [Presentation] Group 2 innate lymphoid cells exacerbate severe amebic liver abscess by IFN-gamma independent manner in mice. (2018)
  • Author(s): Risa Nakamura, Sharmina Deloer, Masachika Senba, Mihoko Kikuchi, Shigeo Koyasu, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第11回寄生虫感染免疫研究会
• [Presentation] Existing schistosome infection suppresses malaria liver burden following sporozoite challenge (2017)
  • Author(s): Taeko Moriyasu, Risa Nakamura, Richard Culleton, Shinjiro Hamano
  • Organizer: Japan-Brazil Malaria Workshop
  • Place of Presentation: University of Sao Paulo（サンパウロ、ブラジル）
  • Year and Date: 2017-03-15
  • Int'l Joint Research
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice (2017)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第10回寄生虫感染免疫研究会
  • Place of Presentation: 徳島大学（徳島県徳島市）
  • Year and Date: 2017-02-09
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice. (2017)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第46回日本免疫学会学術集会
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice. (2017)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: The 5th Annual Meeting of the International Cytokine and Interferon Society
  • Int'l Joint Research
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice. (2017)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第16回あわじしま感染症・免疫フォーラム
  • Int'l Joint Research
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice. (2017)
  • Author(s): 中村梨沙, Sharmina Deloer, 茂呂和世, 濱野真二郎
  • Organizer: 第86回日本寄生虫学会大会
• [Presentation] Type 2 innate lymphoid cells exacerbate severe amebic liver abscess in mice. (2017)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: The 13th Nagasaki-Singapore Medical Symposium / Leading Program International Symposium
  • Int'l Joint Research
• [Presentation] IL-17A may help to persist of Entamoeba histolytica by keeping antagonistic relationship with Th1 response in animal model of intestinal amebiasis. (2017)
  • Author(s): Sharmina Deloer, Risa Nakamura, Mihoko Kikuchi, Masachika Senba, Shinjiro Hamano
  • Organizer: The 13th Nagasaki-Singapore Medical Symposium / Leading Program International Symposium
  • Int'l Joint Research
• [Presentation] IL-17A contributes to the decrease of IFN-γ/IL-4 ratio and the persistence of Entamoeba histolytica during intestinal amebiasis. (2017)
  • Author(s): Sharmina Deloer, Risa Nakamura, Mihoko Kikuchi, Taeko Moriyasu, Yombo Dan Justin Kalenda, Eman Sayed Mohammed, Masachika Senba, Yoichiro Iwakura, Hiroki Yoshida, Shinjiro Hamano
  • Organizer: The 5th Annual Meeting of the International Cytokine and Interferon Society
  • Int'l Joint Research
• [Presentation] IFN-gamma controls amebic liver abscess and the function of type 2 innate lymphoid cells in mice intraportally inoculated with Entamoeba histolytica (2016)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shinjiro Hamano
  • Organizer: 第45回日本免疫学会学術集会
  • Place of Presentation: 沖縄コンベンションセンター（沖縄県宜野湾市）
  • Year and Date: 2016-12-05
• [Presentation] IFN-gamma controls amebic liver abscess and the function of type 2 innate lymphoid cells (2016)
  • Author(s): Risa Nakamura, Sharmina Deloer, Kazuyo Moro, Shigeo Koyasu, Shinjiro Hamano
  • Organizer: American Society of Tropical Medicine and Hygene
  • Place of Presentation: Atlanta Marriott Marquis and Hilton Atlanta（アトランタ、アメリカ）
  • Year and Date: 2016-11-13
  • Int'l Joint Research
• [Book] 小児科臨床ーグローバル化・温暖化と感染症対策 (2017)
  • Author(s): 田中美緒・中村梨沙・濱野真二郎
  • Total Pages: 289
  • Publisher: 日本小児医事出版社
  • ISBN: 4910047281279
• [Book] 医学の歩み 「連載 グローバル感染症最前線 ～NTDsの先へ」感染症の現状 ～３大感染症から新興・再興感染症、NTDs. (2017)
  • Author(s): 森保妙子、中村梨沙、濱野真二郎
  • Total Pages: 208
  • Publisher: 医歯薬出版株式会社
• [Book] 「アメーバ赤痢」、臨床免疫・アレルギー科 (2016)
  • Author(s): 中村梨沙、見市文香、Sharmina Deloer、濱野真二郎
  • Total Pages: 101
  • Publisher: 科学評論社