The mechanisms by which polyI:C enhances IgA production in NALT

• Project/Area Number: 16K19134
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Hokkaido University
• Principal Investigator: Ariki Hiromi 北海道大学, 医学研究科, 助教 (40515061)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
• Keywords: TLR3 / polyI:C / CD103陽性樹状細胞 / 鼻腔関連リンパ組織 / NALT / 樹状細胞 / 粘膜免疫 / TICAM1 / インフルエンザワクチン / ウィルス / 免疫学

Outline of Final Research Achievements

The development of influenza virus prevention vaccines requires the proper selection of appropriate adjuvants. The mechanism by which poly I: C promotes antibody production has not been fully elucidated. PolyI:C is a synthetic analog for double-stranded RNA and intranasal inoculation with polyI:C strongly enhances IgA production in nasal mucosa. To uncover the mechanisms by which intranasal inoculation with polyI:C enhances IgA production, I investigated immune responses in nasal-associated lymphoid tissue after intranasal vaccination.

Research Products

• [Journal Article] The anti-oxidant ergothioneine augments the immunomodulatory function of TLR agonists by direct action on macrophages. (2017)
  • Author(s): Yoshida, S., Shime, H., Funami, K., Takaki, H., Tomiyama, T., Matsumoto, M., Kasahara, M. and Seya, T.
  • Journal Title: PLoS ONE Volume: 12 Issue: 1 Pages: e0169360-e0169360
  • DOI: 10.1371/journal.pone.0169360
  • NAID: 120006219937
  • Peer Reviewed / Open Access
• [Journal Article] Development of mouse models for analysis of human virus infections (2017)
  • Author(s): Takaki Hiromi、Oshiumi Hiroyuki、Shingai Masashi、Matsumoto Misako、Seya Tsukasa
  • Journal Title: Microbiology and Immunology Volume: 61 Issue: 3-4 Pages: 107-113
  • DOI: 10.1111/1348-0421.12477
  • NAID: 40021175296
  • Peer Reviewed
• [Journal Article] Toll-like receptor 3 in nasal CD103+ dendritic cells is involved in immunoglobulin A production (2017)
  • Author(s): Takaki H、Kure S、Oshiumi H、Sakoda Y、Suzuki T、Ainai A、Hasegawa H、Matsumoto M、Seya T
  • Journal Title: Mucosal Immunology Volume: 11 Issue: 1 Pages: 82-96
  • DOI: 10.1038/mi.2017.48
  • Peer Reviewed
• [Journal Article] The TLR3/TICAM-1 signal constitutively controls spontaneous polyposis through suppression of c-Myc in Apc Min/+ mice (2017)
  • Author(s): Ono Junya、Shime Hiroaki、Takaki Hiromi、Takashima Ken、Funami Kenji、Yoshida Sumito、Takeda Yohei、Matsumoto Misako、Kasahara Masanori、Seya Tsukasa
  • Journal Title: Journal of Biomedical Science Volume: 24 Issue: 1 Pages: 79-88
  • DOI: 10.1186/s12929-017-0387-z
  • NAID: 120006370604
  • Peer Reviewed / Open Access
• [Journal Article] cGAMP Promotes Germinal Center Formation and Production of IgA in Nasal-Associated Lymphoid Tissue (2017)
  • Author(s): Takaki Hiromi、Takashima Ken、Oshiumi Hiroyuki、Ainai Akira、Suzuki Tadaki、Hasegawa Hideki、Matsumoto Misako、Seya Tsukasa
  • Journal Title: Medical Sciences Volume: 5 Issue: 4 Pages: 35-35
  • DOI: 10.3390/medsci5040035
  • Peer Reviewed / Open Access
• [Journal Article] Interferon-stimulated gene of 20 kDa protein (ISG20) degrades RNA of Hepatitis B virus to impede the replication of HBV in vitro and in vivo. (2016)
  • Author(s): Leong CR, Funami K, Oshiumi H, Mengao D, Takaki H, Matsumoto M, Aly HH, Watashi K, Chayama K, Seya T.
  • Journal Title: Oncotarget. Volume: 7 Issue: 42 Pages: 68179-68193
  • DOI: 10.18632/oncotarget.11907
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Cytokine responses to eye spray adjuvants for enhancing vaccine-induced immunity in chickens. (2016)
  • Author(s): Hiromi Takaki, Haruko Sato, Riho Kurata, Hirokazu Hikono, Takahiro Hiono, Hiroshi Kida, Misako Matsumoto, Takehiko Saito, Tsukasa Seya
  • Journal Title: Microbiology and Immunology Volume: 60 Issue: 7 Pages: 511-511
  • DOI: 10.1111/1348-0421.12391
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] パターン認識受容体と病原体感知機序 (2016)
  • Author(s): 高木宏美、瀬谷司
  • Journal Title: 日本臨床 Volume: 74 Pages: 1934-1934
• [Presentation] PolyI:C-induced, TLR3/RIP3-dependent necroptosis backs up immune effector-mediated tumor elimination in vivo (2017)
  • Author(s): Hiromi Takaki, Hiroaki Shime, Misako Matsumoto, Tsukasa Seya
  • Organizer: Sapporo International Cancer symposium
  • Int'l Joint Research
• [Presentation] polyI:CはTLR3-TICAM1経路を介してNALTでの抗体産生を促進する (2016)
  • Author(s): 高木宏美、松本美佐子、瀬谷司
  • Organizer: 第64回日本ウイルス学会
  • Place of Presentation: 札幌コンベンションセンター（北海道札幌市）
  • Year and Date: 2016-10-23