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Elucidation of HIV-1 pathogenesis dictated by the interplay between Nef and SERINC3/5

Research Project

Project/Area Number 16K19141
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Virology
Research InstitutionKumamoto University

Principal Investigator

Toyoda Mako  熊本大学, エイズ学研究センター, 研究員 (70771129)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000)
Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
Keywordsウイルス / HIV-1 / SERINC3/5 / Nef / HLA / ウィルス / 感染症
Outline of Final Research Achievements

The host proteins SERINC 3 and 5 have been revealed as inhibitors of HIV-1 infectivity that are counteracted by Nef. However, it remains unclear whether this ability affects viral replication in vivo. Here, we examined the effects of patient-derived sequences on Nef’s ability to counteract SERINC3/5 and its association with patients’ viral load. We collected plasma viral RNA from HLA-typed, treatment-naive, chronically HIV-1 subtype B-infected subjects and determined Nef-encoding region sequences. Correlation between the Nef polymorphisms and plasma viral load was analyzed. We identified two Nef mutations that are correlated inversely with viral load. The Nef’s ability to counteract SERINC3/5 was assessed by using JTAg wild-type cells and the JTAg variant engineered to lack expression of both SERINC3/5. Viruses produced by JTAg, but not SERINC3/5-deleted JTAg, demonstrated that the two Nef mutations markedly impaired SERINC3/5 counteraction function.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (9 results)

All 2018 2017 2016

All Journal Article (1 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results,  Open Access: 1 results) Presentation (8 results) (of which Int'l Joint Research: 5 results)

  • [Journal Article] Resistance of major histocompatibility complex class B (MHC-B) to Nef-mediated downregulation relative to that of MHC-A is conserved among primate lentiviruses and influences antiviral T cell responses in HIV-1-infected individuals.2018

    • Author(s)
      F Mwimanzi, M Toyoda, M Mahiti, J Mann, J Martin, D Bangsberg, M Brockman, P Goulder, F Kirchhoff, Z Brumme, T Ndung’u, T Ueno
    • Journal Title

      Journal of Virology

      Volume: 92 Issue: 1

    • DOI

      10.1128/jvi.01409-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Presentation] Naturally arising HIV-1 Nef variants that impair Nef’s ability to counteract SERINC3/5 are associated with decreased plasma viremia.2017

    • Author(s)
      Toyoda Mako, Kamori Doreen, Carlson Jonathan, Gatanaga Hiroyuki, Kawana-Tachikawa Ai, Oka Shinichi, Pizzato Massimo, Ueno Takamasa.
    • Organizer
      18th Kumamoto AIDS Seminar.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Differential ability to antagonize SERINC3/5 counteraction by naturally arising HIV-1 Nef variants associating with decreased plasma viremia.2017

    • Author(s)
      豊田真子、 Kamori Doreen、Jonathan Carlson、潟永博之、立川(川名)愛、岡慎一、Massimo Pizzato、上野貴将.
    • Organizer
      第65回日本ウイルス学会学術集会.
    • Related Report
      2017 Annual Research Report
  • [Presentation] Impaired Nef’s ability to counteract SERINC5 is associated with decreased plasma viremia.2017

    • Author(s)
      Toyoda Mako, Kamori Doreen, Carlson Jonathan, Gatanaga Hiroyuki, Kawana-Tachikawa Ai, Oka Shinichi, Pizzato Massimo, Ueno Takamasa.
    • Organizer
      9th IAS Conference on HIV Science
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impaired Nef’s ability to counteract SERINC5 is associated with decreased plasma viremia.2017

    • Author(s)
      Toyoda Mako, Kamori Doreen, Carlson Jonathan, Gatanaga Hiroyuki, Kawana-Tachikawa Ai, Oka Shinichi, Pizzato Massimo, Ueno Takamasa.
    • Organizer
      Keystone Symposia Conference.
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] アクセサリー蛋白質に対する免疫淘汰圧がウイルス複製に与える影響2016

    • Author(s)
      豊田真子、 Kamori Doreen、立川(川名)愛、潟永博之、岡慎一、上野貴将
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      鹿児島県民交流センター
    • Year and Date
      2016-11-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Naturally occurring HIV-1 Nef variants that impair Nef’s ability to counteract SERINC5 and enhance virion infectivity.2016

    • Author(s)
      Toyoda Mako, Kamori Doreen, Pizzato Massimo, Ueno Takamasa
    • Organizer
      2nd Kumamoto IRCMS International Symposium and 17th Kumamoto AIDS Seminar.
    • Place of Presentation
      Kumamoto Kenmin Koryu Kaikan-Parea
    • Year and Date
      2016-10-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Viral fitness cost imposed by naturally occurring HLA-associated polymorphisms across HIV-1 accessory proteins.2016

    • Author(s)
      豊田真子、 Kamori Doreen、上野貴将
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] Naturally occurring HLA-associated Nef polymorphisms that are associated with decreased plasma viral load and impaired Nef-mediated enhancement of virion infectivity.2016

    • Author(s)
      Toyoda Mako, Kamori Doreen, Ueno Takamasa
    • Organizer
      Cold Spring Harbor Laboratory Meeting: Retroviruses.
    • Place of Presentation
      NY, USA. Cold Spring Harbor Laboratory
    • Year and Date
      2016-05-23
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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