• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of de novo CTLs in recognition of mutant viruses escaped from HLA-mediated immune pressure

Research Project

Project/Area Number 16K19160
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Immunology
Research InstitutionKumamoto University

Principal Investigator

Akahoshi Tomohiro  熊本大学, エイズ学研究センター, 特定事業研究員 (10635783)

Research Collaborator GATANAGA Hiroyuki  国立国際医療研究センター, エイズ治療・研究開発センター
Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
KeywordsHIV-1 / CTL / HLAクラスⅠ関連変異 / エピトープ推定 / 変異エピトープ / ネオエピトープ / 変異特異的CTL / HLA関連多型 / 感染症 / ウイルス / 細胞・組織 / 免疫学
Outline of Final Research Achievements

In present study, we aimed to clarify to what extent HIV-1-specific CD8+ cytotoxic T cells (CTL) recognizing HLA-adapted escape mutants are induced in a cohort of chronically HIV-1-infeand Japanese individuals and whether they contribute to control of HIV-1 replication. Among mutant epitopes which were predicted based on cohort’s consensus viral sequence containing HLA-associated mutations, we focused on two epitopes in which mixture of wild type (WT) and mutant viruses and/or frequent selection and reversion of those viruses were longitudinally observed. In those epitopes, cross-reactive CTLs, which recognized both WT and mutant peptides, and mutant-specific CTLs were induced in HIV-1-infected individuals. These results indicated that de novo CTLs recognizing HLA-adapted escape mutants elicited in chronic phase of HIV-1 infection may contribute to control of HIV-1 infection.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (37 results)

All 2018 2017 2016 Other

All Journal Article (4 results) (of which Int'l Joint Research: 4 results,  Peer Reviewed: 4 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (31 results) (of which Int'l Joint Research: 16 results) Remarks (2 results)

  • [Journal Article] HLA Class I-Mediated HIV-1 Control in Vietnamese Infected with HIV-1 Subtype A/E2017

    • Author(s)
      Chikata Takayuki、Tran Giang Van、Murakoshi Hayato、Akahoshi Tomohiro、Qi Ying、Naranbhai Vivek、Kuse Nozomi、Tamura Yoshiko、Koyanagi Madoka、Sakai Sachiko、et al.
    • Journal Title

      Journal of Virology

      Volume: 92 Issue: 5

    • DOI

      10.1128/jvi.01749-17

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] Novel, in-natural-infection subdominant HIV-1 CD8+ T-cell epitopes revealed in human recipients of conserved-region T-cell vaccines2017

    • Author(s)
      Nicola Borthwick, Zhansong Lin, Tomohiro Akahoshi, Anuska Llano, Sandra Silva-Arrieta, Tina Ahmed, Lucy Dorrell, Christian Brander, Hayato Murakoshi, Masafumi Takiguchi, Tomas Hanke
    • Journal Title

      PLoS One

      Volume: 12 Issue: 4 Pages: e0176418-e0176418

    • DOI

      10.1371/journal.pone.0176418

    • Related Report
      2017 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] HIV-1 control by NK cells via reduced interaction between KIR2DL2 and HLA-C*12:02/C*14:032016

    • Author(s)
      Lin Z, Kuroki K, Kuse N, Sun X, Akahoshi T, Qi Y, Chikata T, Naruto T, Koyanagi M, Murakoshi H, Gatanaga H, Oka S, Carrington M, Maenaka K, Takiguchi M.
    • Journal Title

      Cell Rep.

      Volume: 17 (9) Issue: 9 Pages: 2210-2220

    • DOI

      10.1016/j.celrep.2016.10.075

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Effects of a Single Escape Mutation on T Cell and HIV-1 Co-adaptation.2016

    • Author(s)
      Sun X, Shi Y, Akahoshi T, Fujiwara M, Gatanaga H, Schönbach C, Kuse N, Appay V, Gao GF, Oka S, Takiguchi M.
    • Journal Title

      Cell Rep.

      Volume: 15 (10) Issue: 10 Pages: 2279-91

    • DOI

      10.1016/j.celrep.2016.05.017

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Presentation] HLA class I-mediated HIV-1 control in HIV-1 infection2018

    • Author(s)
      Masafumi Takiguchi, Hayato Murakoshi, Takayuki Chikata, Tomohiro Akahoshi, Nozomi Kuse, Zhansong Lin, Mary Carrington, Hiroyuki Gatanaga, and Shinichi Oka
    • Organizer
      20th International Conference on Emerging Infectious Diseases in the Pacific Rim/U.S.-Japan Cooperative Medical Sciences Program (Shenzhen, China)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HLA class I-mediated HIV-1 control in Vietnamese infected with HIV-1 subtype A/E2018

    • Author(s)
      Masafumi Takiguchi, Takayuki Chikata, Giang Van Tran, Hayato Murakoshi, Tomohiro Akahoshi, Ying Qi, Vivek Naranbhai, Nozomi Kuse, Yoshiko Tamura, Madoka Koyanagi, Sachiko Sakai, Dung Hoai Nguyen, Dung Thi Nguyen, Ha Thu Nguyen, Trung Vu Nguyen, Shinichi Oka, Maureen P. Martin, Mary Carrington, Keiko Sakai, et al.
    • Organizer
      20th International Conference on Emerging Infectious Diseases in the Pacific Rim/U.S.-Japan Cooperative Medical Sciences Program (Shenzhen, China)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impact of a single escape mutation selected by HLA-A*24:02-restricted CD8+ T cells on HIV-1 control by HLA-B*35:01-restricted ones and T cell adaptation2017

    • Author(s)
      Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Nozomi Kuse, and Masafumi Takiguchi
    • Organizer
      第46回日本免疫学会学術集会(仙台)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Effective priming of functional HIV-1-specific CD8+ T Cells from naive T cells using STING ligand2017

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Takuya Yamamoto, Victor Appay, and Masafumi Takiguchi
    • Organizer
      第46回日本免疫学会学術集会(仙台)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 強いHIV-1増殖抑制能を有したCTLによる変異HIV-1の認識2017

    • Author(s)
      村越 勇人, 久世 望, 赤星 智寛, 近田 貴敬, Zhang Yu, 潟永 博之, 岡 慎一, 滝口 雅文
    • Organizer
      第31回日本エイズ学会学術集会・総会(東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] 複数の異なったHLA拘束性CTLによるRT135変異の選択とその相互作用2017

    • Author(s)
      赤星 智寛, 久世 望, 村越 勇人, 近田 貴敬, 潟永 博之, 岡 慎一, 滝口 雅文
    • Organizer
      第31回日本エイズ学会学術集会・総会(東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] ベトナム人HIV-1サブタイプA/E感染者コホートにおける、エイズ病態進行に関与するHLAアリルおよびHLA関連HIV-1多型のエイズ病態進行に及ぼす影響2017

    • Author(s)
      近田 貴敬, Tran Van Giang, 村越 勇人, 田村 美子, 赤星 智寛, 久世 望, 阪井 恵子, 小柳 円, Kinh Van Nguyen, 滝口 雅文
    • Organizer
      第31回日本エイズ学会学術集会・総会(東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] STINGリガンドによるウイルス抑制機能の高いHIV-1特異的CD8陽性T細胞のプライミング2017

    • Author(s)
      久世 望, Sun Xiaoming, 赤星 智寛, Lissina Anna, 山本 拓也, Appay Victor, 滝口 雅文
    • Organizer
      第31回日本エイズ学会学術集会・総会(東京)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Control of HIV-1 by CTLs restricted by an HLA-B*52:01-HLA-C*12:02 protective haplotype2017

    • Author(s)
      Hayato Murakoshi, Takayuki Chikata, Madoka Koyanagi, Kazutaka Honda, Lin Zhansong, Tomohiro Akahoshi, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      第65回日本ウイルス学会学術集会(大阪)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Identification of a detrimental HLA haplotype and the effect of its associated HIV-1 mutations in HIV-1 subtype A/E-infected Vietnamese2017

    • Author(s)
      Takayuki Chikata, Hayato Murakoshi, Tomohiro Akahoshi, Nozomi Kuse, Yoshiko Tamura, Keiko Sakai, and Masafumi Takiguchi
    • Organizer
      第65回日本ウイルス学会学術集会(大阪)
    • Related Report
      2017 Annual Research Report
  • [Presentation] Control of HIV-1 by an HLA-B*52:01-HLA-C*12:02 protective haplotype2017

    • Author(s)
      Hayato Murakoshi, Takayuki Chikata, Madoka Koyanagi, Kazutaka Honda, Tomohiro Akahoshi, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] STING ligand effectively prime functional HIV-1-specific CD8+ T Cells from naive T cells2017

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Takuya Yamamoto, Victor Appay, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Generation of HIV-1 polymorphism at RT135 via coevolution of multiple HIV-1-specific T cells with HIV-12017

    • Author(s)
      Tomohiro Akahoshi, Nozomi Kuse, Hayato Murakoshi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] HLA class I-mediated HIV-1 control in Vietnamese infected with HIV-1 subtype A/E2017

    • Author(s)
      Takayuki Chikata, Giang Van Tran, Hayato Murakoshi, Tomohiro Akahoshi, Nozomi Kuse, Ying Qi, Yoshiko Tamura, Madoka Koyanagi, Sachiko Sakai, Dung Hoai Nguyen, Dung Thi Nguyen, Ha Thu Nguyen, Trung Vu Nguyen, Shinichi Oka, Marry Carrington, Keiko Sakai, Kinh Van Nguyen, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The Effect of KIR Genes on HIV-1 Control in the Vietnamese Cohort2017

    • Author(s)
      Zhansong Lin, Takayuki Chikata, Giang Van Tran, Hayato Murakoshi, Tomohiro Akahoshi, Nozomi Kuse, Yoshiko Tamura, Madoka Koyanagi, Sachiko Sakai, Dung Hoai Nguyen, Dung Thi Nguyen, Ha Thu Nguyen, Trung Vu Nguyen, Keiko Sakai, Kinh Van Nguyen, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Analysis of conserved epitopes-specific and cross-reactive CTLs controlling HIV-1 in Japanese individuals2017

    • Author(s)
      Yu Zhang, Nozomi Kuse, Hayato Murakoshi, Tomohiro Akahoshi, Takayuki Chikata, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      18th Kumamoto AIDS Seminar (Kumamoto, Japan)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Impact of a single escape mutation selected by HLA-A*24:02-restricted CD8+ T cells on HIV-1 control by HLA-B*35:01-restricted ones and T cell adaptation2017

    • Author(s)
      Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Katherine L James, Yoshiko Tamura, Nozomi Kuse, Xiaoming Sun, Hiroyuki Gatanaga, Sarah L Rowland-Jones, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      IAS 2017 (Paris, France)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Effective priming of CD8+ T cells with ability to suppress HIV-1 replication using STING ligand2017

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Ken J. Ishii, Takuya Yamamoto, Victor Appay, and Masafumi Takiguchi
    • Organizer
      IAS 2017 (Paris, France)
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] The effect of one amino acid difference outside of peptide binding groove of HLA-C molecules on NK cell function and HIV-1 control2016

    • Author(s)
      Zhansong Lin, Nozomi Kuse, Tomohiro Akahoshi, Kimiko Kuroki, Katsumi Maenaka, and Masafumi Takiguchi
    • Organizer
      第45回日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄・宜野湾市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
  • [Presentation] In vitro priming of HIV-1-specific CD8+ T cells from naive T cells2016

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Takuya Yamamoto, Ken J. Ishii, Victor Appay, and Masafumi Takiguchi
    • Organizer
      第45回日本免疫学会学術集会
    • Place of Presentation
      沖縄コンベンションセンター(沖縄・宜野湾市)
    • Year and Date
      2016-12-05
    • Related Report
      2016 Research-status Report
  • [Presentation] The effect of a single CTL escape -mutation on HIV-1 control by NK cell functions2016

    • Author(s)
      Zhansong Lin, Nozomi Kuse, Tomohiro Akahoshi, Kimiko Kuroki, Katsumi Maenaka, and Masafumi Takiguchi
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県・鹿児島市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
  • [Presentation] ベトナム人HIV-1サブタイプA/E感染者コホートにおけるエイズ病態進行に関与するHLAアリルの探索2016

    • Author(s)
      近田 貴敬, Giang Van Tran, 村越 勇人, 田村 美子, 赤星 智寛, 久世 望, 阪井 恵子, 小柳 円, Kinh Van Nguyen, 滝口 雅文
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県・鹿児島市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
  • [Presentation] HIV-1と特異的CTLの相互適応2016

    • Author(s)
      赤星 智寛, 端本 昌夫, 村越 勇人, 近田 貴敬, 田村 美子, 潟永 博之, 岡 慎一, 滝口 雅文
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県・鹿児島市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Impact of a Single HLA-A*24:02-associated Escape Mutation on HLA-B*35:01-associated HIV-1 Control and T cell Adaptation2016

    • Author(s)
      Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Katherine L James, Yoshiko Tamura, Nozomi Kuse, Xiaoming Sun, Hiroyuki Gatanaga, Sarah L Rowland-Jones, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      第30回日本エイズ学会学術集会・総会
    • Place of Presentation
      かごしま県民交流センター(鹿児島県・鹿児島市)
    • Year and Date
      2016-11-24
    • Related Report
      2016 Research-status Report
  • [Presentation] Control of HIV-1 by NK cells via KIR-2DL2 and 2 HLA-C alleles2016

    • Author(s)
      Zhansong Lin, Kimiko Kuroki, Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Ying Qi, Takayuki Chikata, Takuya Naruto, Madoka Koyanagi, Hayato Murakoshi, Hiroyuki Gatanaga, Shinichi Oka, Mary Carrington, Katsumi Maenaka, Masafumi Takiguchi
    • Organizer
      2nd Kumamoto IRCMS International Symposium and 17th Kumamoto AIDS Seminar
    • Place of Presentation
      くまもと県民交流館パレア(熊本県・熊本市)
    • Year and Date
      2016-10-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Contribution of HLA class I alleles to clinical outcome in HIV-1 subtype A/E infected Vietnamese individuals2016

    • Author(s)
      Takayuki Chikata, Giang Van Tran, Hayato Murakoshi, Yoshiko Tamura, Tomohiro Akahoshi, Nozomi Kuse, Madoka Koyanagi, Sachiko Sakai, Keiko Sakai, Kinh Van Nguyen, and Masafumi Takiguchi
    • Organizer
      2nd Kumamoto IRCMS International Symposium and 17th Kumamoto AIDS Seminar
    • Place of Presentation
      くまもと県民交流館パレア(熊本県・熊本市)
    • Year and Date
      2016-10-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Co-evolution between HIV-1 escape mutant and HIV-1-specific T cells2016

    • Author(s)
      Tomohiro Akahoshi, Masao Hashimoto, Takayuki Chikata, Hayato Murakoshi, Nozomi Kuse, Yoshiko Tamura, Hiroyuki Gatanaga, Shinichi Oka, and Masafumi Takiguchi
    • Organizer
      2nd Kumamoto IRCMS International Symposium and 17th Kumamoto AIDS Seminar
    • Place of Presentation
      くまもと県民交流館パレア(熊本県・熊本市)
    • Year and Date
      2016-10-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Functional analysis of HIV-1-specific CD8+ T cells primed in vitro from naive T cells2016

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Victor Appay, and Masafumi Takiguchi
    • Organizer
      2nd Kumamoto IRCMS International Symposium and 17th Kumamoto AIDS Seminar
    • Place of Presentation
      くまもと県民交流館パレア(熊本県・熊本市)
    • Year and Date
      2016-10-31
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Contribution of HLA class I alleles to clinical outcome in HIV-1 subtype A/E infected Vietnamese individuals2016

    • Author(s)
      Takayuki Chikata, Giang Van Tran, Hayato Murakoshi, Yoshiko Tamura, Tomohiro Akahoshi, Nozomi Kuse, Keiko Sakai, Kinh Van Nguyen, and Masafumi Takiguchi
    • Organizer
      第64回日本ウイルス学会学術集会
    • Place of Presentation
      札幌コンベンションセンター(北海道・札幌市)
    • Year and Date
      2016-10-23
    • Related Report
      2016 Research-status Report
  • [Presentation] In Vitro Priming of HIV-1-specific CD8+ T Cells from Naive T Cells2016

    • Author(s)
      Nozomi Kuse, Xiaoming Sun, Tomohiro Akahoshi, Anna Lissina, Victor Appay, and Masafumi Takiguchi
    • Organizer
      HIVR4P 2016
    • Place of Presentation
      Sheraton Grand Chicago(Chicago, USA)
    • Year and Date
      2016-10-17
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Presentation] Impaired Effect of HLA-A*24:02-Associated Mutation on HLA-B*35:01-Associated HIV-1 Control2016

    • Author(s)
      Hayato Murakoshi, Tomohiro Akahoshi, Madoka Koyanagi, Takayuki Chikata, Katherine L James, Nozomi Kuse, Yoshiko Tamura, Sarah L Rowland-Jones, Shinichi Oka, Hiroyuki Gatanaga, and Masafumi Takiguchi
    • Organizer
      HIVR4P 2016
    • Place of Presentation
      Sheraton Grand Chicago(Chicago, USA)
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research
  • [Remarks] 熊本大学エイズ学研究センター 滝口プロジェクト研究室

    • URL

      http://www.caids.kumamoto-u.ac.jp/data/takiguchi/default.html

    • Related Report
      2017 Annual Research Report
  • [Remarks] 熊本大学エイズ学研究センター滝口プロジェクト研究室 研究業績

    • URL

      http://www.caids.kumamoto-u.ac.jp/data/takiguchi/viral/gyouseki/gyousekitop.htm

    • Related Report
      2016 Research-status Report

URL: 

Published: 2016-04-21   Modified: 2019-03-29  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi