| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2018: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
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| Outline of Final Research Achievements |
Mice having CD4+ cell-specific deletion of Rap1 (Rap1KO mice) developed spontaneous colitis. In this study, we investigated the involvement of innate lymphocytes (γδ T cells and ILC3) in the development of colitis. To examine the roles of γδ T cells for colitis, we crossed Rap1KO mice with TCRδ-/- mice (DKO mice). The reduction in weight and histological scores of colitis were suppressed in DKO mice until 12 weeks of age. These data suggest that γδ T cells play critical role in the promotion of colitis at early phase of onset. Since previous study suggests that ILC3 cells have an important role of intestinal immunity, we examined the cell number and function of ILC3 cells. There was no difference in the cell number and IL-22 production of CD4+ILC3 cells derived from LI-LP and mLN between control and Rap1KO mice before the onset of colitis. These data indicate that ILC3 cells are not involved in the development of colitis.
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