| Project/Area Number |
16K19202
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Kitasato University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
SHICHIRI Masayoshi 北里大学, 医学部, 教授 (10206097)
KODERA Yoshio 北里大学, 理学部, 教授 (60265733)
HAYASHI Akinori 北里大学, 医学部, 助教 (30458830)
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| Research Collaborator |
KAMATA Yuji
OGAWA Akifumi
KATO Yukiko
KAWASHIMA Yusuke
SAITO Tatsuya
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
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| Keywords | ELISA / 生理活性ペプチド / ELISA系 / サンドイッチELISA / EIA / バイオマーカー |
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| Outline of Final Research Achievements |
Salusin-β was initially predicted from bioinformatic analyses in 2003. Salusin-β acutely regulates hemodynamics and chronically induces atherosclerosis, but its unique physicochemical characteristics to tightly adhere to all types of plastic and glassware have prevented elucidation of its precise pathophysiological role. In this study, we successfully established a sandwich enzyme-linked immunosorbent assay (ELISA) to quantitate plasma free full-length salusin-β concentrations. Free salusin-β levels showed circadian variation and its levels in patients with diabetes mellitus and panhypopituitarism combined with complete central diabetes insipidus showed significantly lower than healthy controls.
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