Effect of antimicrobial peptide cathelicidin on atopic dermatitis

• Project/Area Number: 16K19217
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Pain science
• Research Institution: Juntendo University
• Principal Investigator: Umehara Yoshie 順天堂大学, 医学研究科, 博士研究員 (40707072)
• Research Collaborator: KAMATA Yayoi 順天堂大学, 大学院・医学研究科, 助教 (00410035) ; MATSUDA Hironori 順天堂大学, 大学院・医学研究科, 技術員 ; NIYONSABA Francois 順天堂大学, 国際教養学部, 教授 (60365640) ; TOMINAGA Mitsutoshi 順天堂大学, 大学院・医学研究科, 准教授 (50468592)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2016: ¥2,210,000 (Direct Cost: ¥1,700,000、Indirect Cost: ¥510,000)
• Keywords: 難治性かゆみ / アトピー性皮膚炎 / 抗菌ペプチド / カテリシジン / アトピー性皮膚炎モデルNC/Ngaマウス / ドライスキン / かゆみ / Sema3A

Outline of Final Research Achievements

We investigated the effect of antimicrobial peptide cathelicidin on atopic dermatitis (AD). Following induction of AD-like symptoms on NC/Nga mice, cathelicidin mCRAMP ointment was applied to the lesional skin. Although dermatitis scores and scratching behavior were not suppressed, stratum corneum hydration was improved by the ointment. We also found that expressions of calpain I and BH mRNA in epidermis were increased. These data suggest that increased NMFs by upregulation of NMF-generating proteases improve stratum corneum hydration.

Research Products

• [Journal Article] Antimicrobial peptides human LL-37 and β-defensin-3 modulate the expression of nerve elongation factors in human epidermal keratinocytes (2017)
  • Author(s): Umehara Yoshie、Kamata Yayoi、Tominaga Mitsutoshi、Niyonsaba Fran?ois、Ogawa Hideoki、Takamori Kenji
  • Journal Title: Journal of Dermatological Science Volume: 88 Issue: 3 Pages: 365-367
  • DOI: 10.1016/j.jdermsci.2017.07.010
  • Peer Reviewed
• [Journal Article] Involvement of μ-opioid Receptors and κ-opioid Receptors in Itch-related Scratching Behaviour of Imiquimod-induced Psoriasis-like Dermatitis in Mice (2017)
  • Author(s): Takahashi N, Tominaga M, Kosaka R, Kamata Y, Umehara Y, Matsuda H, Sakaguchi A, Ogawa H, Takamori K
  • Journal Title: Acta Dermato Venereologica Volume: 97 Issue: 8 Pages: 928-933
  • DOI: 10.2340/00015555-2704
  • Peer Reviewed
• [Journal Article] Oral administration of milk-derived phospholipids inhibits penetration of cutaneous nerve fibres into epidermis in a mouse model of acute dry skin (2017)
  • Author(s): Sakaguchi A, Kamata Y, Takahashi N, Matsuda H, Kosaka R, Umehara Y, Ogawa H, Tominaga M, Takamori K
  • Journal Title: Clinical and Experimental Dermatology Volume: 42 Issue: 8 Pages: 890-894
  • DOI: 10.1111/ced.13207
  • Peer Reviewed
• [Journal Article] Differences in therapeutic effects of topically applied corticosteroid and tacrolimus on atopic dermatitis-like symptoms in NC/Nga mice. (2016)
  • Author(s): Atsushi Noguchi, Mitsutoshi Tominaga, Nobuaki Takahashi, Hironori Matsuda, Yayoi Kamata, Yoshie Umehara, Ko Kyi Chan, Yasushi Suga, Hideoki Ogawa, Kenji Takamori
  • Journal Title: J Dermatol Sci Volume: 86 Issue: 1 Pages: 54-62
  • DOI: 10.1016/j.jdermsci.2016.12.015
  • Peer Reviewed
• [Journal Article] Relationships among plasma granzyme B level, pruritus and dermatitis in patients with atopic dermatitis. (2016)
  • Author(s): Kamata Y, Kimura U, Matsuda H, Tengara S, Kamo A, Umehara Y, Iizumi K, Kawasaki H, Suga Y, Ogawa H, Tominaga M, Takamori K.
  • Journal Title: J Dermatol Sci Volume: 84 Issue: 3 Pages: 266-271
  • DOI: 10.1016/j.jdermsci.2016.09.009
  • Peer Reviewed
• [Journal Article] Neurotropin suppresses itch-related behavior in NC/Nga mice with atopic dermatitis-like symptoms. (2016)
  • Author(s): Kamo A, Tominaga M, Matsuda H, Kina K, Kamata Y, Umehara Y, Ogawa H, Takamori K.
  • Journal Title: J Dermatol Sci Volume: 81 Issue: 3 Pages: 212-215
  • DOI: 10.1016/j.jdermsci.2015.11.014
  • Peer Reviewed
• [Journal Article] Possible anti-pruritic mechanisms of cyclosporine A in atopic dermatitis. (2016)
  • Author(s): Ko KC, Tominaga M, Kamata Y, Umehara Y, Matsuda H, Takahashi N, Kina K, Ogawa M, Ogawa H, Takamori K.
  • Journal Title: Acta Derm Venereol Volume: 未定 Issue: 5 Pages: 624-629
  • DOI: 10.2340/00015555-2318
  • Peer Reviewed / Open Access
• [Presentation] A method to differentiate peripheral neurons from human induced pluripotent stem cells to develop treatments for intractable itch (2017)
  • Author(s): Yoshie Umehara, Mitsutoshi Tominaga, Hironori Matsuda, Nobuaki Takahashi, Yayoi Kamata, Hideoki Ogawa, Kenji Takamori
  • Organizer: 日本研究皮膚科学会 第42回年次学術大会・総会
• [Presentation] Calcium increases semaphorin 3A expression by activating PKC/MAPK/AP-1 signaling axis in normal human epidermal keratinocytes (2017)
  • Author(s): Yayoi Kamata, Yoshie Umehara, Azumi Sakaguchi, Yasushi Suga, Hideoki Ogawa, Mitsutoshi Tominaga, Kenji Takamori
  • Organizer: 日本研究皮膚科学会 第42回年次学術大会・総会
• [Presentation] 乳由来リン脂質の経口投与は急性ドライスキンモデルマウスにおける表皮内神経線維の増生を抑制する (2017)
  • Author(s): 坂口 安澄、鎌田 弥生、高橋 伸明、松田 浩則、幸坂 涼平、梅原 芳恵、須賀 康、冨永 光俊、髙森 建二
  • Organizer: 第47回日本皮膚アレルギー・接触皮膚炎学会総会学術大会
• [Presentation] Sema3A expression is regulated by calcium/PKC/MAPK/AP-1 signaling axis in normal human epidermal keratinocytes (2017)
  • Author(s): Yayoi Kamata, Yoshie Umehara, Azumi Sakaguchi, Yasushi Suga, Mitsutoshi Tominaga, Kenji Takamori
  • Organizer: 9th World Congress of Itch
  • Int'l Joint Research
• [Presentation] Effects of antimicrobial peptide LL-37 on expression of natural moisturizing factor-generating proteases in epidermal keratinocytes (2016)
  • Author(s): Yoshie Umehara, Yayoi Kamata, Mitsutoshi Tominaga, Francois Niyonsaba, Hideoki Ogawa, Kenji Takamori
  • Organizer: 日本研究皮膚科学会 第 41 回年次学術大会・総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-12-09
• [Presentation] Serum lipocalin-2 is a potential biomarker for pruritus in patients with psoriasis (2016)
  • Author(s): Mitsutoshi Tominaga, Nobuaki Takahashi, Utako Kimura, Yayoi Kamata, Yoshie Umehara, Yasushi Suga, Hideoki Ogawa, Kenji Takamori
  • Organizer: 日本研究皮膚科学会 第 41 回年次学術大会・総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-12-09
• [Presentation] Involvement of opioid systems in itch-related behavior of imiquimod-induced psoriasis-like dermatitis model (2016)
  • Author(s): Nobuaki Takahashi, Mitsutoshi Tominaga, Yayoi Kamata, Yoshie Umehara, Hironori Matsuda, Yasushi Suga, Hideoki Ogawa, Kenji Takamori
  • Organizer: 日本研究皮膚科学会 第 41 回年次学術大会・総会
  • Place of Presentation: 仙台
  • Year and Date: 2016-12-09
• [Presentation] ドラッグリポジショニングに向けたセマフォリン3A発現調節剤のin vitroスクリーニング (2016)
  • Author(s): 鎌田弥生、冨永光俊、坂口安澄、梅原芳恵、髙森建二
  • Organizer: 第89回日本生化学会大会
  • Place of Presentation: 仙台
  • Year and Date: 2016-09-25
• [Remarks] 研究：髙森グループ
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kankyo_igaku/k4_takamori.html
• [Remarks] かゆみと真剣勝負、かゆみの克服を目指して！
  • URL: http://www.juntendo.ac.jp/graduate/laboratory/labo/kankyo_igaku/kayumi/