| Project/Area Number |
16K19219
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Pain science
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| Research Institution | Kansai Medical University |
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Principal Investigator |
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000)
Fiscal Year 2017: ¥2,080,000 (Direct Cost: ¥1,600,000、Indirect Cost: ¥480,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 痒み / NMDA受容体 / GluN2B / 脊髄 / NR2B / NMDAR / c-fos / 全脳イメージング / 脳 |
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| Outline of Final Research Achievements |
Although itch and pain are intimately related and may share similar peripheral and central mechanisms, there is substantial evidence that itch and pain are transmitted via their own distinct pathways. By using mice with a knock-in mutation of the Tyr1472 site to phenylalanine of GluN2B (Y1472F-KI), we found that the phosphorylation of GluN2B subunit is important for itch transmission for various stimuli including histamine and chloroquine, although serotonin itch was transmitted by distinct pathways. To distinguish neuronal pathways in the spinal cord for each pain and itch stimulus, we conducted experiments using Tet-on/off systems or double staining with immunohistochemistry and In-situ hybridization. We also searched for brain regions concerning itch processing. We made chronic itch mouse model and analyzed c-fos expression globally in the full brain.
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