Effect of environmental factors during pregnancy on lipid metabolism and growth among of children through epigenetic modification

• Project/Area Number: 16K19245
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Epidemiology and preventive medicine
• Research Institution: Hokkaido University
• Principal Investigator: Miyashita Chihiro 北海道大学, 環境健康科学研究教育センター, 特任准教授 (70632389)
• Project Period (FY): 2016-04-01 – 2019-03-31
• Project Status: Completed (Fiscal Year 2018)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2018: ¥780,000 (Direct Cost: ¥600,000、Indirect Cost: ¥180,000); Fiscal Year 2017: ¥1,690,000 (Direct Cost: ¥1,300,000、Indirect Cost: ¥390,000); Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
• Keywords: 疫学 / エピゲノム / 肥満度 / 分子遺伝学 / 分子遺伝疫学

Outline of Final Research Achievements

Associations between concentrations of fatty acids in maternal blood during pregnancy (log2) and the obesity index of elementary school children were evaluated in the regression analysis adjusted by maternal BMI, smoking and alcohol drinking during pregnancy, and inshore fish intake. The obesity index of 5th and 6th grade elementary school children were significantly decreased with increased concentration of maternal palmitoleic acid. Associations between DNA methylations of IGF-2, H19 and LINE-1 in cord blood (log2) and the obesity index of elementary school children were evaluated in the regression analysis. No obesity index of children was associated with DNA methylations. This study suggests that DNA modification of IGF-2, H19 and LINE-1 at birth may slightly affect on associations between maternal fatty acid during pregnancy on growth of elementary school children.

Academic Significance and Societal Importance of the Research Achievements

本研究は母の妊娠中の栄養や環境と，その児の小学生の肥満度との関係に，臍帯血中DNAメチル化率がどのように関与するか国内で初めて検討した。胎児期や生後の早期の栄養および環境が成長後の児の発育や疾病発生に影響を及ぼす生体機序に関して，後天的遺伝子修飾であるDNAメチル化はその生体機序の一つであると示唆されている。しかしヒト集団を用いて疫学データとDNAメチル化率との関連を検討した報告はわずかで，本研究はヒトのDNAメチル化と生体影響について貴重な知見を提供した。

Research Products

• [Journal Article] Sex-related differences in the associations between maternal dioxin-like compounds and reproductive and steroid hormones in cord blood: The Hokkaido study. (2018)
  • Author(s): Miyashita C, Araki A, Mitsui T, Itoh S, Goudarzi H, Sasaki S, Kajiwara J, Hori T, Cho K, Moriya K, Shinohara N, Nonomura K, Kishi R.
  • Journal Title: Environ Int Volume: 117 Pages: 175-185
  • DOI: 10.1016/j.envint.2018.04.046
  • NAID: 120006876269
  • Peer Reviewed / Open Access
• [Journal Article] Prenatal organochlorine pesticide exposure and the disruption of steroids and reproductive hormones in cord blood: The Hokkaido study (2018)
  • Author(s): Araki Atsuko、Miyashita Chihiro、Mitsui Takahiko、Goudarzi Houman、Mizutani Futoshi、Chisaki Youichi、Itoh Sachiko、Sasaki Seiko、Cho Kazutoshi、Moriya Kimihiko、Shinohara Nobuo、Nonomura Katsuya、Kishi Reiko
  • Journal Title: The Hokkaido study. Environ Int. Volume: 110 Pages: 1-13
  • DOI: 10.1016/j.envint.2017.10.006
  • NAID: 120006776798
  • Peer Reviewed / Open Access
• [Journal Article] An epigenome-wide study of cord blood DNA methylations in relation to prenatal perfluoroalkyl substance exposure: The Hokkaido study (2018)
  • Author(s): Miura Ryu、Araki Atsuko、Miyashita Chihiro、Kobayashi Sumitaka、Kobayashi Sachiko、Wang Shu-Li、Chen Chung-Hsing、Miyake Kunio、Ishizuka Mayumi、Iwasaki Yusuke、Ito Yoichi M.、Kubota Takeo、Kishi Reiko
  • Journal Title: Environment International Volume: 115 Pages: 21-28
  • DOI: 10.1016/j.envint.2018.03.004
  • NAID: 120006847752
  • Peer Reviewed
• [Journal Article] Predictors of folate status among pregnant Japanese women: the Hokkaido Study on Environment and Children's Health, 2002-2012. (2016)
  • Author(s): Yila TA, Araki A, Sasaki S, Miyashita C, Itoh K, Ikeno T, Yoshioka E, Kobayashi S, Goudarzi H, Baba T, Braimoh T, Minakami H, Endo T, Sengoku K, Kishi R.
  • Journal Title: Br J Nutr Volume: 115 Issue: 12 Pages: 2227-2235
  • DOI: 10.1017/s0007114516001628
  • NAID: 120006219876
  • Peer Reviewed / Open Access
• [Presentation] 胎児期ダイオキシン類曝露による第二次性徴への影響：環境と子供の健康北海道スタディ. (2017)
  • Author(s): 宮下ちひろ，荒木敦子，三井貴彦，伊藤佐智子，梶原淳睦，堀就英, 長和俊，守屋仁彦, 篠原信雄，岸玲子
  • Organizer: 第69回北海道公衆衛生学会; 2017.11.18; 札幌.
• [Presentation] ダイオキシン類異性体の曝露が胎生期の性ホルモンに与える影響-北海道スタディ（The Hokkaido Study on Environment and Children's Health) (2016)
  • Author(s): 45)宮下ちひろ, 荒木敦子, 三井貴彦, 伊藤佐智子, 佐々木成子, 戸高尊, 梶原淳睦, 長和俊, 野々村克也, 岸玲子
  • Organizer: 第86回日本衛生学会
  • Place of Presentation: 旭川市民文化会館（北海道旭川市）
  • Year and Date: 2016-05-11
• [Remarks] 北海道大学 環境健康科学研究教育センター 研究業績
  • URL: https://www.cehs.hokudai.ac.jp/research/yearly/
• [Remarks] 北海道大学環境健康科学研究教育センター 研究業績
  • URL: https://www.cehs.hokudai.ac.jp/research/yearly/original-articles-reviews-wpeer/
• [Remarks] 北海道大学環境健康科学研究教育センター 研究業績
  • URL: https://www.cehs.hokudai.ac.jp/research/yearly/