Investigation of obesity-related metabolic markers in plasma and urine using metabolome profiling

• Project/Area Number: 16K19259
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Epidemiology and preventive medicine
• Research Institution: Keio University
• Principal Investigator: Kurihara Ayako 慶應義塾大学, 医学部(信濃町), 助教 (40645470)
• Research Collaborator: Takebayashi Toru ; Harada Sei ; Takeuchi Ayano ; Okamura Tomonori ; Sugiyama Daisuke ; Iida Miho
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2017: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000); Fiscal Year 2016: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
• Keywords: 内臓脂肪蓄積量 / 肥満 / メタボロームプロファイリング / メタボローム解析 / インスリン抵抗性 / 地域コホート研究 / メタボローム / コホート研究

Outline of Final Research Achievements

As a result of PLS discriminant analysis, the plasma metabolites related to the following four amino acid metabolic pathways contributed to obese distinction: glycine, serine and threonine metabolism, cysteine and methionine metabolism, phenylalanine metabolism, histidine metabolism. As for urine metabolites, two amino acid metabolic pathways were associated with obesity: phenylalanine metabolism, alanine, aspartate and glutamate metabolism. Metabolites of phenylalanine metabolism were commonly related to obesity in plasma and urine. The plasma lipid metabolites also contributed to obese distinction, especially regarding fatty acid biosynthesis, linoleic acid metabolism, biosynthesis of unsaturated fatty acids. We will continue with further examination while following the study subjects.

Research Products

• [Journal Article] Reliability of plasma polar metabolite concentrations in a large-scale cohort study using capillary electrophoresis-mass spectrometry (2018)
  • Author(s): Harada Sei、Hirayama Akiyoshi、Chan Queenie、Kurihara Ayako、Fukai Kota、Iida Miho、Kato Suzuka、Sugiyama Daisuke、Kuwabara Kazuyo、Takeuchi Ayano、Akiyama Miki、Okamura Tomonori、Ebbels Timothy M. D.、Elliott Paul、Tomita Masaru、Sato Asako、Suzuki Chizuru、Sugimoto Masahiro、Soga Tomoyoshi、Takebayashi Toru
  • Journal Title: PLOS ONE Volume: 13 Issue: 1 Pages: e0191230-e0191230
  • DOI: 10.1371/journal.pone.0191230
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Presentation] Plasma metabolome associated with obesity in community dwellers: Tsuruoka metabolomic cohort study (2017)
  • Author(s): Ayako Kurihara et al.
  • Organizer: IUNS 21st International Congress of Nutrition
• [Remarks] 鶴岡みらい健康調査
  • URL: http://tsuruoka-mirai.net/