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Mitochondrial DNA typing in mixtures using next generation sequencing system

Research Project

Project/Area Number 16K19301
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Legal medicine
Research InstitutionTokai University

Principal Investigator

OCHIAI Eriko  東海大学, 医学部, 助教 (60760270)

Project Period (FY) 2016-04-01 – 2018-03-31
Project Status Completed (Fiscal Year 2017)
Budget Amount *help
¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
Fiscal Year 2017: ¥910,000 (Direct Cost: ¥700,000、Indirect Cost: ¥210,000)
Fiscal Year 2016: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000)
Keywords混合試料 / ミトコンドリアDNA / 次世代シーケンス / DNA鑑定 / 遺伝子
Outline of Final Research Achievements

Analysis of "DNA mixtures" in which DNAs of more than one person are mixed is one of important issues in forensic field, but no technical method for separating DNA types of individuals has been established yet. Therefore, mitochondrial (mt) DNA analysis using the next generation sequencer (NGS) was performed, and attempts were made to separate multiple DNA types by comparing differences in base sequence of DNA chain (read) for each molecule. When NGS analysis was performed with the amplification product of about 350 bp, the entire amplification region of both HV1 and HV2 could be detected with one read. A plurality of nucleotide polymorphisms was confirmed on the obtained read, and it was possible to distinguish different mtDNA type patterns from the combination and to be judged as a DNA type of two persons.

Report

(3 results)
  • 2017 Annual Research Report   Final Research Report ( PDF )
  • 2016 Research-status Report
  • Research Products

    (14 results)

All 2018 2017 2016

All Journal Article (5 results) (of which Int'l Joint Research: 1 results,  Peer Reviewed: 1 results) Presentation (9 results) (of which Int'l Joint Research: 3 results)

  • [Journal Article] 混合試料のミトコンドリアDNA型判定における次世代シーケンサーの応用2018

    • Author(s)
      落合恵理子,松島裕,水口清,垣内康宏,垣本由布,大澤資樹
    • Journal Title

      DNA多型

      Volume: 26

    • Related Report
      2017 Annual Research Report
  • [Journal Article] 次世代シーケンサーを用いた死後遺伝学的検査:突然死例に対する循環器系疾患関連遺伝子解析2017

    • Author(s)
      落合恵理子,松島裕,垣本由布,大澤資樹
    • Journal Title

      DNA多型

      Volume: 25 Pages: 164-166

    • Related Report
      2017 Annual Research Report
  • [Journal Article] Evaluation of Y chromosomal SNP haplogrouping in the HID-Ion AmpliSeqTM Identity Panel.2016

    • Author(s)
      Ochiai E, Minaguchi K, Nambiar P, Kakimoto Y, Satoh F, Nakatome M, Miyashita K, Osawa M
    • Journal Title

      Leg Med (Tokyo)

      Volume: 22 Pages: 58-61

    • DOI

      10.1016/j.legalmed.2016.08.001

    • Related Report
      2016 Research-status Report
    • Peer Reviewed / Int'l Joint Research
  • [Journal Article] 次世代シーケンサーを用いたY染色体ハプログループ解析2016

    • Author(s)
      落合恵理子,宮下京子,水口清,垣本由布,佐藤文子,大澤資樹
    • Journal Title

      DNA多型

      Volume: 24 Pages: 169-171

    • Related Report
      2016 Research-status Report
  • [Journal Article] 次世代シークエンサーを利用したSNP解析の血縁関係推定への応用2016

    • Author(s)
      宮下京子,落合恵理子,田村友紀,大澤資樹
    • Journal Title

      DNA多型

      Volume: 24 Pages: 200-203

    • Related Report
      2016 Research-status Report
  • [Presentation] Mitochondrial DNA typing in mixtures using massive parallel sequencing2018

    • Author(s)
      Ochiai E, Matsushima Y, Minaguchi K, Kakiuchi Y, Kakimoto Y, Osawa M
    • Organizer
      24th Congress of the International Academy of Legal Medicine
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 次世代シーケンサーを用いた死後遺伝学的検査:循環器系疾患解析パネルの評価2017

    • Author(s)
      落合恵理子,垣本由布,瀬戸良久,坪井秋男,松島裕,大澤資樹
    • Organizer
      第101次日本法医学会学術全国集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたY-SNPハプログループ解析2017

    • Author(s)
      大澤資樹,落合恵理子,水口清,垣本由布
    • Organizer
      日本アンドロロジー学会第36回学術大会
    • Related Report
      2017 Annual Research Report
  • [Presentation] Postmortem genetic testing using the next generation sequencing system: Evaluation of cardiovascular disease analysis panel2017

    • Author(s)
      Ochiai E, Kakimoto Y, Seto Y, Tsuboi A, Matsushima Y, Osawa M
    • Organizer
      10th International Symposium Advances in Legal Medicine
    • Related Report
      2017 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 骨様および軟組織様断片のDNA鑑定2017

    • Author(s)
      水口清,落合恵理子,松島裕,大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 日本人の姓とY染色体DNA多型の関連性2017

    • Author(s)
      佐藤栞,落合恵理子,水口清,垣本由布,大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 混合試料のミトコンドリアDNA型判定における次世代シーケンサーの応用2017

    • Author(s)
      落合恵理子,松島裕,水口清,垣内康宏,垣本由布,大澤資樹
    • Organizer
      日本DNA多型学会第26回学術集会
    • Related Report
      2017 Annual Research Report
  • [Presentation] 次世代シーケンサーを用いたY-SNP解析とハプログループ判定2016

    • Author(s)
      落合恵理子,水口清,垣本由布,大澤資樹
    • Organizer
      日本法科学技術学会第22回学術集会
    • Place of Presentation
      中野サンプラザ(東京都中野区)
    • Year and Date
      2016-11-10
    • Related Report
      2016 Research-status Report
  • [Presentation] Y chromosome haplogrouping using the next generation sequencing system2016

    • Author(s)
      Ochiai E, Miyashita K, Minaguchi K, Kakimoto Y, Satoh F, Osawa M
    • Organizer
      ICHG2016 The13th International Congress of Human Genetics
    • Place of Presentation
      Kyoto International Conference Center (Kyoto, Japan)
    • Year and Date
      2016-04-03
    • Related Report
      2016 Research-status Report
    • Int'l Joint Research

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Published: 2016-04-21   Modified: 2019-03-29  

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