Changes with the time of the amount of physiologically active substance in the tissue: potential application to forensic diagnosis
| Project/Area Number |
16K19303
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Legal medicine
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| Research Institution | Osaka Medical College |
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Principal Investigator |
Saito Takashi 大阪医科大学, 医学部, 助教 (40764981)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2016: ¥2,470,000 (Direct Cost: ¥1,900,000、Indirect Cost: ¥570,000)
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| Keywords | 法医診断 / 死後変化 / 経時変化 / 脂肪幹細胞 / 表面マーカー / 脂肪由来間葉系幹細胞 / 脂肪組織 / 休眠状態 / 脂肪 / 間葉系幹細胞 / 死後経過時間 / 死後経過時間の推定 |
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| Outline of Final Research Achievements |
Although postmortem stem cells may be useful for regenerative therapy and forensic diagnostics, their characteristic remain to be better understood. Adipose-derived mesenchymal stem cells (ASCs) have the capacity to differentiate through several cell lineages and are able to survive in an ischemic environment for a prolonged time. This study aimed to confirm whether human postmortem ASCs can be collected and culture-expanded from cadavers. Axilla subcutaneous adipose tissues were harvested during forensic autopsy and enzymatically digested to obtain a heterogeneous cell mixture, including the ASCs population. The mixture was seeded onto collagen-coated cell culture dishes and spindle-shaped adhesive and proliferative ASCs were confirmed. And then, the cultured cells were characterized.
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Report
(3 results)
Research Products
(5 results)
