Pancreatic disease screening and grade evaluation system using metabolomics with blood and pancreatic juice

• Project/Area Number: 16K19342
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Kobe University
• Principal Investigator: KOBAYASHI Takashi 神戸大学, 医学研究科, 医学研究員 (90709669)
• Project Period (FY): 2016-04-01 – 2018-03-31
• Project Status: Completed (Fiscal Year 2017)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000); Fiscal Year 2017: ¥1,560,000 (Direct Cost: ¥1,200,000、Indirect Cost: ¥360,000); Fiscal Year 2016: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: pancreatic cancer / metabolomics / screening / biomarker / メタボロミクス / IPMN / 膵がん / バイオマーカー / 質量分析 / 血液 / 膵液 / MS

Outline of Final Research Achievements

The aim of this study was to establish pancreatic disease screening and grade evaluation system using metabolomics with blood and pancreatic juice. Blood samples were collected by 2 independent groups. In the 1st set (55 patients and 58 controls), Model Y, consisting of histidine and xylitol, possessed higher sensitivity (70.4%) than CA19-9. In the 2nd set (16 patients and 16 controls), model Y with CA19-9 displayed high sensitivity (81.3%) and specificity (93.8%). In particular, it displayed very high sensitivity　(100%) for resectable PC.
These quantitative analysis confirmed that metabolomics-based diagnostic methods are useful for detecting pancreatic cancer early.

Research Products

• [Journal Article] Metabolome analysis for pancreatic cancer risk in nested case-control study: Japan Public Health Center-based prospective Study (2018)
  • Author(s): Nakagawa T, Kobayashi T, Nishiumi S, Hidaka A, Yamaji T, Sawada N, Hirata Y, Yamanaka K, Azuma T, Goto A, Shimazu T, Inoue M, Iwasaki M, Yoshida M, Tsugane S
  • Journal Title: Cancer Sci. Volume: 25 Issue: 5 Pages: 1672-1681
  • DOI: 10.1111/cas.13573
  • NAID: 120006482610
  • Peer Reviewed / Open Access
• [Journal Article] Identification of highly sensitive biomarkers that can aid the early detection of pancreatic cancer using GC/MS/MS-based targeted metabolomics. (2016)
  • Author(s): Hirata Y., Ioka T., et al.
  • Journal Title: Clin. Chim. Acta. Volume: Feb. 16 Pages: 98-104
  • DOI: 10.1016/j.cca.2017.02.011
  • NAID: 120006375910
  • Peer Reviewed / Open Access
• [Presentation] 血液メタボロミクスによる膵がんスクリーニング法の開発 (2018)
  • Author(s): 小林 隆、平田 祐一、吉田 優
  • Organizer: 日本消化器病学会総会
• [Presentation] Validation of serum/plasma metabolomic biomarkers against pancreatic cancer by quantitative targeted GC/MS/MS (2017)
  • Author(s): Takashi Kobayashi, Yuichi Hirata, Masaru Yoshida, Shin Nishiumi, Kazufumi Honda
  • Organizer: UEGW2018
  • Int'l Joint Research
• [Book] 臨床消化器内科 (2016)
  • Author(s): 小林 隆, 久津見 弘, 八木 洋輔, 酒井 新, 吉中 勇人, 西海 信, 吉田 優, 東 健
  • Total Pages: 6
  • Publisher: 日本メディカル