| Project/Area Number |
16K19347
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | The University of Tokushima |
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Principal Investigator |
KITAMURA Shinji 徳島大学, 大学院医歯薬学研究部(医学系), 助教 (60564490)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,170,000 (Direct Cost: ¥900,000、Indirect Cost: ¥270,000)
Fiscal Year 2016: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
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| Keywords | 直腸カルチノイド / カルチノイド / miRNA |
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| Outline of Final Research Achievements |
We performed miRNA array analysis using 7 NET-G1 tumors with LVI and 7 without LVI. Identified miRNA expression level was evaluated by RT-PCR. Target genes were examined by IPA and TargetScan analyses. A NET cell line H727 transfected with miRNA mimic or target gene siRNA was examined for proliferation, migration and invasion.The expression levels of miR-144-3p and miR-451a in NET-G1 tumors with LVI were significantly higher than those without LVI with miRNA array analysis and RT-PCR. The target gene analyses revealed that miR-144-3p and miR-451a directly interact with PTEN and p19 mRNA, respectively. The miR-451a mimic significantly increased cell migration, and miR-144-3p mimic significantly increased cell invasion. Knockdown of PTEN induced significant augmentation of cell invasion. The miR-144-3p and miR-451a may be associated with metastasis in NET-G1 through repression of PTEN/p19.
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