| Project/Area Number |
16K19368
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Gastroenterology
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| Research Institution | Tokai University |
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Principal Investigator |
ANZAI Kazuya 東海大学, 医学部, 助教 (20724941)
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| Project Period (FY) |
2016-04-01 – 2018-03-31
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| Project Status |
Completed (Fiscal Year 2017)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2017: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
Fiscal Year 2016: ¥1,950,000 (Direct Cost: ¥1,500,000、Indirect Cost: ¥450,000)
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| Keywords | 胆管前駆細胞 / 肝前駆細胞 / 分化誘導 / 胎生期肝前駆細胞 / 再生医学 / 細胞・組織 |
|---|
| Outline of Final Research Achievements |
In this study, we demonstrated that cholangiocytic progenitor cells could be induced from foetal LPCs by culturing them on gelatine-coated dishes for 7days. But this induction was significantly suppressed by addition of HGF or OSM, these are maturation factor for hepatic linage, to the pre-culture conditions. Because these cholangiocitic progenitor cells show similar characteristics to neonatal LPCs, that are matured in vivo for 7 days, in vitro inductions in 2D culture are equivalent to in vivo development. Moreover, our culture system could be useful for analysis of the intrinsic changes of foetal LPCs towards cholangiocytic differentiation. We demonstrated that the overexpression of Grhl2 lead to the improvement of cholangiocytic cyst formation.
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